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EMAGE:16786

Crlf3 cytokine receptor-like factor 3 ( MGI:1860086)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786
"Pseudo-wholemount" of euxassay_008617. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008617_01 euxassay_008617_02 euxassay_008617_03 euxassay_008617_04
EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786
euxassay_008617_05 euxassay_008617_06 euxassay_008617_07 euxassay_008617_08 euxassay_008617_09
EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786
euxassay_008617_10 euxassay_008617_11 euxassay_008617_12 euxassay_008617_13 euxassay_008617_14
EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786
euxassay_008617_15 euxassay_008617_16 euxassay_008617_17 euxassay_008617_18 euxassay_008617_19
EMAGE:16786 EMAGE:16786 EMAGE:16786 EMAGE:16786
euxassay_008617_20 euxassay_008617_21 euxassay_008617_22 euxassay_008617_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16786Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16786_wholemount_strong.wlz
16786_wholemount_moderate.wlz
16786_wholemount_weak.wlz
16786_wholemount_possible.wlz
16786_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16786_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 13 14 moderate expression: see section 11 12
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1312
Entity Detected:Crlf3, cytokine receptor-like factor 3 ( MGI:1860086)
Sequence:sense strand is shown

>T1312
TCGAGCCTGTTGGCCTACTGGAGTCGGGAATCCGGCGGGCCGCGCGACCAGATGAAGGGCGCGATGGAGC
CGGAGCCCGAGGTGCTGCTTCAGGAAGCCCGCGAGAACGTGGAGGCGGCGCAGAGCTACCGGCGGGAGCT
GGGCCAGCGGCTGCAGGGGCTGCGGGAGGCGCAGAGGCAGATAAAGGAGAGTGCTTCACAGACAAGAGAT
GTCCTCAAACAGCATTTTAGTGATTTAAAAGGAACCCTTGGGAAGCTATTGGATGAGCGATTGGTGACCC
TTTTGCAAGAGGTGGACACCATTGAACAGGAGACCATCAAACCACTAGATGACTGCCAGAAACTCATAGA
GCATGGGGTCAACACTGCAGATGACCTGGTCCGAGAAGGTGAGATTGCCATTCTTGGTGGTATCGAAGAA
GAGAGTGATAAACTGTGGAACTTTACCAAAAAGGCCTCACACATTCAGCTGGACAGCTTACCAGAAGTGC
CCTTGCTAGTTGATGTACCCTGTTTGTCGGCTCAGTTGGACGATTCCATTCTTAACATAGTGAAAGACCA
Notes:The probe template was PCR amplified from IMAGE:2259281 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2259281 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4824061 same experiment
 EurExpress:euxassay_008617 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS