EMAGE mouse gene expression spatial database

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EMAGE:16840

Mylpf myosin light chain, phosphorylatable, fast skeletal muscle ( MGI:97273)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008765. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008765_01	euxassay_008765_02	euxassay_008765_03	euxassay_008765_04

euxassay_008765_05	euxassay_008765_06	euxassay_008765_07	euxassay_008765_08	euxassay_008765_09

euxassay_008765_10	euxassay_008765_11	euxassay_008765_12	euxassay_008765_13	euxassay_008765_14

euxassay_008765_15	euxassay_008765_16	euxassay_008765_17	euxassay_008765_18	euxassay_008765_19

euxassay_008765_20	euxassay_008765_21	euxassay_008765_22	euxassay_008765_23	euxassay_008765_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16840_wholemount_strong.wlz

16840_wholemount_moderate.wlz

16840_wholemount_weak.wlz

16840_wholemount_possible.wlz

16840_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16840_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24

arm

strong

regional

strong expression: see section 01 02 not examined expression: see section 22

hand

strong

regional

strong expression: see section 01 02 03 20 21 22

foot

strong

regional

strong expression: see section 06 07 20 21 22

leg

strong

regional

strong expression: see section 01 02 03 04 05

head mesenchyme

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 12 13 14 15 16 17 moderate expression: see section 11

tail mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1261
Entity Detected:	Mylpf, myosin light chain, phosphorylatable, fast skeletal muscle ( MGI:97273)
Sequence:	sense strand is shown >T1261 CCTCGAGNCTGTTGGCCTACTGGAGATTCTCTTCCTTTTTCCATCTGGAGCTACTGCCTTGCCCCCAGGA GATCTAAGACATGGCACCCAAGAAGGCCAAGAGAAGGGCAGGAGCGGAAGGGAGCTCCAACGTCTTCTCC ATGTTTGACCAGACTCAGATCCAGGAGTTCAAGGAGGCGTTCACTGTAATTGATCAGAACAGGGATGGCA TTATCGACAAAGAGGATCTTCGGGACACCTTTGCAGCCATGGGCCGTCTCAATGTGAAGAATGAGGAACT CGACGCTATGATGAAGGAAGCCAGTGGGCCCATCAACTTCACTGTCTTCCTGACCATGTTTGGGGAAAAG CTTAAGGGTGCGGACCCGGAGGATGTGATCACTGGAGCCTTCAAGGTCCTGGACCCAGAAGGGAAGGGCA CCATCAAAAAGCAGTTCTTGGAGGAGCTGCTTACCACGCAGTGCGACCGATTTTCCCAGGAGGAGATCAA GAACATGTGGGCAGCCTTCCCCCCAGACGTGGGCGGCAACGTGGACTACAAGAACATCTGCTACGTCATC ACACAT
Notes:	The probe template was PCR amplified from IMAGE:2225544 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225544 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16842 same embryo
	EMAGE:16843 same embryo
	EMAGE:16841 same embryo
	EurExpress:euxassay_008765 same experiment
	MGI:4826555 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source