EMAGE mouse gene expression spatial database

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EMAGE:17000

Ptprcap protein tyrosine phosphatase, receptor type, C polypeptide-associated protein ( MGI:97811)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008819. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008819_01	euxassay_008819_02	euxassay_008819_03	euxassay_008819_04

euxassay_008819_05	euxassay_008819_06	euxassay_008819_07	euxassay_008819_08	euxassay_008819_09

euxassay_008819_10	euxassay_008819_11	euxassay_008819_12	euxassay_008819_13	euxassay_008819_14

euxassay_008819_15	euxassay_008819_16	euxassay_008819_17	euxassay_008819_18	euxassay_008819_19

euxassay_008819_20	euxassay_008819_21	euxassay_008819_22	euxassay_008819_23	euxassay_008819_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17000_wholemount_strong.wlz

17000_wholemount_moderate.wlz

17000_wholemount_weak.wlz

17000_wholemount_possible.wlz

17000_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17000_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2937
Entity Detected:	Ptprcap, protein tyrosine phosphatase, receptor type, C polypeptide-associated protein ( MGI:97811)
Sequence:	sense strand is shown >T2937 TGGCCTCGAGCCAGATTCGGACGAGGCTCAGATTTGGGGTGCTGATGGCCCTGCCAGGGGCACTGGCCTC TGGGGCAGACCCAGAGGACGGTGTGGGCTCCAGTGTGGTCACCATTGTCCTGCTGCTCTTGCTCCTGCTG CTGCTGGTCACTGCCCTAGCCTTGGCTTGGCGTCGCCTCAGCCATGCCTCAGGGGGGTACTACCACCCAG CTCGCCTGGGTGCTGCCTTGTGGGGCCACACCTGCCGCCTGCTCTGGGCCAGCCCTGCAGGCCGCTGGCT TCGCGCCCGCACTGAGCTGGAATCCCCAGAAGAATCAGGGCCGCCGGAGGATGAAGAGGATGCAGAAGAT TTTGTGATAGATGGTGGCCCCGAGGAAGCTGCCGCCAAGGAAGAGGAGCAGCGGTGTCAAGCAGAGCAGA CCCGAGATCCACGTGACACAGACAGTGACGGGGGCCTGGGCCTGAGCTCTCAGGGTCCTGTGGGCTCAGG CAGCAGCGCCGAGGCCCTGCTGAGTGACCTGCATGCCTTTTCTGGTAGTGCTGCCTGGGATGACAGCGCT GGAG
Notes:	The probe template was PCR amplified from IMAGE:1531755 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1531755 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17001 same embryo
	EMAGE:17002 same embryo
	EurExpress:euxassay_008819 same experiment
	MGI:4827526 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source