EMAGE mouse gene expression spatial database

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EMAGE:17153

Nxt1 NTF2-related export protein 1 ( MGI:1929619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001534. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001534_01	euxassay_001534_02	euxassay_001534_03	euxassay_001534_04

euxassay_001534_05	euxassay_001534_06	euxassay_001534_07	euxassay_001534_08	euxassay_001534_09

euxassay_001534_10	euxassay_001534_11	euxassay_001534_12	euxassay_001534_13	euxassay_001534_14

euxassay_001534_15	euxassay_001534_16	euxassay_001534_17	euxassay_001534_18	euxassay_001534_19

euxassay_001534_20	euxassay_001534_21	euxassay_001534_22	euxassay_001534_23	euxassay_001534_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17153_wholemount_strong.wlz

17153_wholemount_moderate.wlz

17153_wholemount_weak.wlz

17153_wholemount_possible.wlz

17153_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17153_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 08 17 18 19

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 17

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

renal cortex

moderate

regional

moderate expression: see section 08 09 10 11 19 20 21

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4739
Entity Detected:	Nxt1, NTF2-related export protein 1 ( MGI:1929619)
Sequence:	sense strand is shown >T4739 CGGCGCGCGGGAAGCCAGTACCGGCGCTTAACCGTTCCTCGCCTCCTGGACGTCGGCTTCTCAGGCGGAG CCTCTGGGAGGAAGAGGAGAACAGGAGAGAAACTCTTTTGGCCCGGGACCGCGACCGGGGCGGAACCCGG GACCGAGTCGCCTTGGCGGCGCTGACCGAGATCGAGCAGTCCCTGGATCCCTAAGGAGGAGGCGCTCAGG CGGAGGCCTCTTGTCAGCAGAACCAGAGATGGCATCAGTAGATTTCAAGACCTATGTGGACCAGGCCTGC AGAGCTGCAGAGGAATTTGTCAATGTCTATTACACGACTATGGATAAGCGGCGTCGGCTGCTGTCCCGCC TGTACATGGGCACAGCCACTTTAGTATGGAATGGAAATGCTGTTTCAGGACAAGAATCCTTGAGTGAGTT TTTTGAGATGTTGCCTTCCAGTGAGTTCCAAATCAGCGTAGTAGACTGCCAGCCTGTCCATGATGACGCT ACACCAAGCCAGACCACCGTGCTTGTGGTCATCTGTGGAACAGTGAAGTTTGAAGGCAACAAACAGCGGG ACTTCAACCAGAACTTCATCTTGACTGCTCAGGCGTCACCCAGTAACACAGTGTGGAAGATAGCAAGTGA CTGCTTCCGGTTCCAGGACTGGGCCAGCTAGTGGGGGTGACCGAGGCATCTTAGCTTAAGCCCAGCTCTG CAGAGAAATGCCAGTCTCAAATCTCAGGATGTGGAGAACACAAGTTCATTTTTGTTGTCACAGCAGCACT GCAGACTGGATGTTCGACTCCCAGAATCCCCATTTCCTGGTTATATACTGGTTTGTCATAGTTGCCTTTT AAAAGTAGTAAACTTTCTATTTTTCTACTTACTCAGTAGAGACCCTGTTTCTGGAACTTGACAAATAAAT ATGTTGCTTTTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5039139 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5039139 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17154 same embryo
	EMAGE:17152 same embryo
	EurExpress:euxassay_001534 same experiment
	MGI:4826843 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source