EMAGE mouse gene expression spatial database

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EMAGE:17154

Kri1 KRI1 homolog (S. cerevisiae) ( MGI:2384899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001530. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001530_01	euxassay_001530_02	euxassay_001530_03	euxassay_001530_04

euxassay_001530_05	euxassay_001530_06	euxassay_001530_07	euxassay_001530_08	euxassay_001530_09

euxassay_001530_10	euxassay_001530_11	euxassay_001530_12	euxassay_001530_13	euxassay_001530_14

euxassay_001530_15	euxassay_001530_16	euxassay_001530_17	euxassay_001530_18	euxassay_001530_19

euxassay_001530_20	euxassay_001530_21	euxassay_001530_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17154_wholemount_strong.wlz

17154_wholemount_moderate.wlz

17154_wholemount_weak.wlz

17154_wholemount_possible.wlz

17154_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17154_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 weak expression: see section 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4740
Entity Detected:	Kri1, KRI1 homolog (S. cerevisiae) ( MGI:2384899)
Sequence:	sense strand is shown >T4740 CCACGCGTCCGGCCGGAACCGCGCGCCTCTGGGTGCCTAAAGGTGAACGCAGCGTTTGCTGCGCGCTACA GCCGTTACCGCGAGCGTGAGGAACTTCAGCGACTGAAAGATCGCTATGGAGATCCGGACGTCGGTGGCGA CTCCAGTTCCGAGTCTGACTCCAGCGATGAGCATGTGGAATTTGATCCCCAGCAGGAGAGAGACTTTTAC AGGACTCTGTCCTTGCTGAAGAAGAAGGACCCTCGAATTTACCAGAAAGATGCTACCTTCTATCAGAGAA CAGCCGAAGCCTCATCTTCAGAGAGTGAGGAGGAGCCGCCAGCAGCCTTGGGGAAGCAGGAGAAGCAGCA GAAGCAGCAGCCCATGTACCTGAAGGACTATGAGAGGAAGGTCATCTTGGAGAAAGGAGGCAAATATGTG GACGAGGACAACTCTGATGGGGAGACCGTTGACCACAGGCTCCAGGAAACATCCACAAAGAGTTATGTAG AAGAGCAGAAACAGCTGAAGGAAAGCTTCCGGGCTTTTGTGGAGGACAGCAGTGACGAGGACAGTGCCGG GGAAGGTGGCTCAGGGTTGCTGCAGAAGCACACTAAGAGTCGGGAAGAGAAGGCCCAGGAGGAAGTGGAC TACCTTGAGTGGCTAAAGGGGCAAAAGGACATGGACAGCTCCGAGTCGCTGAAAGAACTGACACACCTGA AGGAATACTGGAACAACCCAGGACTGGACGAGGGGGAACAGTTCCTGAGGGATTATATCCTCAACAAACG CTATGAGGAGGAGGAGGAGGAAGAGGAGGAAGAGGAGGAGGAAGGGGCTCCAGGCCCCCCAGTCCAGCTG GCAGTGGACGACTCCTCAGATGAAGGGGAGCTATTCCTGAAGAAGCAGGAAGACTTTGAACACAAGTACA ACTTCCGCTTTGAGGAGCCTGACTCAGCCTCAGTCAAGACATACCCACGAAGCATCGCATCATCTGTGCG CCGCAAAGATGAGCGCAGGAAGGAGAAGCGAGAAGAGACGAGGGAGCGGAAGAAGAGGGAGAAGGCCAGG AAGCAGGAGGAGCTGAAACAGTTAAAGAACCTAAAGAGGAAGGAGATTCTGGCCAAGCTGGAGAAGCTGC GACAGGCCACTGGTAACGAGACCCTGGGCCTTGAGGAGCAGGACCTGGAGGCTGACTTTGACCCCGCCCA GCACGACCAGCTCATGCAGAAATGTTTTGGAGATGCCTTCTATGGTACTGCGGAGGAAGAGAAGCCACAG TTTGAGGAAGAGGACGGGCTAGAAGATGACTGGAACTGGGACATGTGGGGTGGACCCGAGCAAGATGGGG CCTGGAGCCAACAGGAGCTGCACTGTGAGGACCCTGACTTTAACATGGATGCAGACTATGACCCCAGCCA ACCACGGAAGAAGCTTCGTGAGGCCCCCACCTCAGGCAAGAGAAAGCGCAAGTCTCCATTTGCAGCTGCA GTGGGGCAGGAGAAGCCCATGTTTGACCCTGGGGACAAGACCTTTGAGGAGTACCTAGATGAGTACTACC GTCTGGACTACGAGGACATTATCGATGACCTGCCCTGTCGTTTCAAGTACCGCACTGTGGTGCCCTGCGA CTTCGGGCTCAGCACAGAGGAGATCCTTTCAGCTGATGACAAGGAACTGAATCGCTGGTGTTCCCTGAAG AAGACCTGCATGTACAGGTCGGAGCAGGAGGAGATGCAGGAGCAGCGGGTGTACAGCCAGAAGGCCCAGA ACATGTGGAAGAAGAGGCAGATCTTCAAGTCTCTGTGCCAGGAAGAGATGGAAATGTCCACAGAAGCCAC AGGAAAGTCACAGAGCAAGGCCAGCCCACAAGTACAGTTGCCCACACCCAATGGAGCTGACCAGAGGACA CCCCAGGCTGAGACCTTAGTATCCAAGGAAGAGGGGTTGGCCCACACATCCTGCCCAGAGAAGCCAGCAT CCCAGAAGCAGAAGAGCAAGAAGGCCAGGCTGCTGGGTCCCACGGTGACACTTGGAGGTCACAAGTTCAG CAGACAGAGATTGCAGGCCTTTGGCCTCAACCCCAAAAGGCTGCACTTCCGCCAGTTAGGCCGCCAACGG AAGAAACAGCAGAGCCACTCCTGAGGGCCACAGGGAGCCAACCTGCCTCTACAACTAAGCTGCTGACCAA ACTCTGCCTGGCAGCCTACGAAAGCTACCTGCAGGAGAGGGCACTGCTGTTCCTAAGCTTTGAAATGAGG CTGCCAGAGCACTGTCTTCTCCACAGACAACCTGAAATGTCAACAACGCCAGGCATGTTCAAGTCTGCAT CTTTATCAGCCACAGTCAGTTCTCCCAGGACAGGACCTTTGGGACCTTGGGACCAGTGACCCAATGCAGG CTACAGAACTTGAATTAGGCCCTGAGTGAGTGCCCTGGAGCCCACTCTCCATCCCTGGCTCCTGAGGAGG CAGGGGCAGCTGGGGGCCCAGATAGGTCTCAGCTGTGTTGGGGAGAACGGGGACATGAGGACAGGGGTCC AGCACCACCACTGGAGTTGCAGATCCCATACCTGACATGACATAAATAAAAAAATAAATAGTCAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5039543 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5039543 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17153 same embryo
	EMAGE:17152 same embryo
	EurExpress:euxassay_001530 same experiment
	MGI:4825821 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source