EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17295

Smox spermine oxidase ( MGI:2445356)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003732_01	euxassay_003732_02	euxassay_003732_03	euxassay_003732_04	euxassay_003732_05

euxassay_003732_06	euxassay_003732_07	euxassay_003732_08	euxassay_003732_09	euxassay_003732_10

euxassay_003732_11	euxassay_003732_12	euxassay_003732_13	euxassay_003732_14	euxassay_003732_15

euxassay_003732_16	euxassay_003732_17	euxassay_003732_18	euxassay_003732_19	euxassay_003732_20

euxassay_003732_21	euxassay_003732_22	euxassay_003732_23	euxassay_003732_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17295_wholemount_strong.wlz

17295_wholemount_moderate.wlz

17295_wholemount_weak.wlz

17295_wholemount_possible.wlz

17295_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17295_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5718
Entity Detected:	Smox, spermine oxidase ( MGI:2445356)
Sequence:	sense strand is shown >T5718 GGCGGCTGCAGGAGGAAGCCCAGTGGCTGCGGAGGAGGGGAGGCGGAGAGGCCGAGGCCGCTCGCGAAGA CGCTCCCGGGCTCCGCAAGGAAAGGCTCCTAGAAGGTGAGCACGGACGGCATGCAAAGTTGTGAATCCAG TGGCGACAGTGCGGATGACCCTCTCAGTCGTGGCCTACGGAGAAGGGGACAGCCTCGTGTGGTGGTGATC GGTGCTGGCTTGGCTGGCCTGGCTGCAGCTAGAGCCCTTCTGGAGCAGGGCTTCACGGATGTCACTGTGC TTGAGGCTTCCAGCCACATTGGGGGCCGTGTGCAGAGTGTGAGGCTTGGAGACACCACCTTTGAGCTGGG AGCCACCTGGATCCATGGATCCCACGGGAATCCTATCTATCAACTAGCAGAAGCCAATGGCCTTTTGGAA GAGACAACAGATGGGGAGCGCAGTGTGGGCCGCATCAGCCTTTACTCCAAGAATGGCGTGGCCTGCTACC TTACCAACCGTGGCTGCCGCATCCCCAAGGACGTGGTTGAGGAATTCAGCGATTTATACAACGAGGTCTA TAACATGACCCAGGAGTTCTTCCGGCATGGTAAACCAGTCAATGCCGAGAGTCAGAACAGCGTCGGGGTG TTCACCCGGGAGAAGGTGCGGAATCGCATCAGGGATGACCCTGACGACACAGAGGCCACCAAGCGCCTGA AGCTCGCCATGATCCAGCAGTACCTGAAGGTGGAGAGCTGTGAGAGCAGCTCCCACAGCATAGATGAGGT GTCCCTGAGCGCCTTTGGAGAATGGACGGAGATCCCAGGCGCCCATCACATCATCCCCTCGGGCTTCATG CGAGTTGTGGAGCTGCTGGCTGAGGGCATTCCTCCACATGTCATCCAGTTGGGGAAGCCGGTCCGTTGCA TCCACTGGGACCAGGCCTCGGCTCACCCCCGGGGTCCTGAGATCGAGCCCCGTGGTGAGGGTGATCACAA TCACGACACTGGGGAGGGTGGCCAGAGTGGAGAGAATCCGCAGCAGGGGAGGTGGGACGAGGATGAGCCG TGGCCTGTAGTCGTGGAGTGCGAGGATTGCGAGGTGATCCCAGCGGACCACGTGATTGTGACCGTTTCGC TGGGCGTGCTCAAGAGGCAGTACACCAGTTTCTTTAGGCCATGCCTGCCCACGGAGAAGGTGGCCGCCAT CCACCGCCTGGGCATTGGTACCACTGACAAGATCTTCCTTGAATTTGAGGAGCCCTTTTGGGGCCCCGAG TGCAACAGCCTGCAGTTCGTGTGGGAGGATGAGGCAGAGAGCTGTACCCTCACCTACCCACCTGAGCTCT GGTACCGCAAGATCTGTGGCTTCGATGTCCTTTATCCGCCAGAGCGCTATGGCCATGTGCTGAGTGGCTG GATCTGTGGGGAGGAGGCTCTTGTCATGGAGAGGTGCGATGACGAGGCTGTAGCTGAGATCTGCACAGAG ATGCTTCGACAGTTCACAGGGAACCCCAATATACCAAAACCTAGGCGAATCCTGCGCTCAGCCTGGGGCA GCAACCCATACTTCCGGGGTTCCTATTCCTACACACAGGTGGGCTCAAGTGGGGCGGATGTGGAGAAGCT AGCCAAGCCCCTGCCCTACACAGAGAGCTCCAAGACAGCGCCCATGCAGGTGCTCTTCTCCGGGGAGGCC ACACACCGCAAGTACTACTCCACCACCCACGGTGCTCTGCTCTCTGGCCAGCGCGAGGCCGCCCGGCTCA TCGAGATGTACCGAGACCTCTTCCAGCAGGGGCCCTGAAAGGTGTCCTCACTGCCAAATGTGTTCCTTGA AGAACCACTAACTCGTGACTGCTGGTCCTCGTCCCTTGCCCCCGTGCTCTTAATTTCCTAATTGTCTGTA GAATGGAATTTTACCTTCTGTAGTGGACACCGGGCTGACGTCAGACCTGGCCCGAGAGCTTTTCTTTTTC TCCAGGCTGGGTAATGACCAGGTGGGGTTCTGCTTCCTGCTTTTTTCCTCTCTCATGTTATTGCAAGTGC CTTCAATACTTTGCATTTTGGGATAATAAAAGAAGTGACCCTTCCCACGCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2647695 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647695 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17294 same embryo
	EMAGE:17293 same embryo
	EurExpress:euxassay_003732 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source