EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17320

Csnk1g2 casein kinase 1, gamma 2 ( MGI:1920014)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003717. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003717_01	euxassay_003717_02	euxassay_003717_03	euxassay_003717_04

euxassay_003717_05	euxassay_003717_06	euxassay_003717_07	euxassay_003717_08	euxassay_003717_09

euxassay_003717_10	euxassay_003717_11	euxassay_003717_12	euxassay_003717_13	euxassay_003717_14

euxassay_003717_15	euxassay_003717_16	euxassay_003717_17	euxassay_003717_18	euxassay_003717_19

euxassay_003717_20	euxassay_003717_21	euxassay_003717_22	euxassay_003717_23	euxassay_003717_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17320_wholemount_strong.wlz

17320_wholemount_moderate.wlz

17320_wholemount_weak.wlz

17320_wholemount_possible.wlz

17320_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17320_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5731
Entity Detected:	Csnk1g2, casein kinase 1, gamma 2 ( MGI:1920014)
Sequence:	sense strand is shown >T5731 GGAGCCGGAGCGGGGGCCCGAGCCGGAGCGCGAACGTGCGCGGGGCCCGGAGCGGGGGACGCCGCGCTGC TGAGCTCGGCCACCGCCGCCCGCGGGCCCCAGGCGGGGCGCAGCTGGGCACCACAAGATTTAAGGCAGTG GGACCTGTTGGCAGAATGTCTTCTCTCTCTGCAAGCAACCCTGAAGCCACCAAGATGAGCACCCCAGTCC GGCCTGTGAGCGTACCCGGGTGTCAATAGCCGGGAGCCGAGGGGCCACCGGGAGCAATGGTGTACTAGGC CGGGCGCGAGCTCAGGTTTCGCACTCGGATCTCACGGCAGAGTCACCGTGGAGAGGCCAGGGTACCACAA ACTTATGGATTTTGACAAGAAAGGAGGGAAGGGGGAGTTGGAGGAGGGCAGGAGAATGTCCAAAACCGGC ACGAGTCGGAGCAACCACGGCGTCCGCAGTTCGGGGACCAGCTCAGGGGTCCTGATGGTGGGCCCAAACT TCCGAGTCGGCAAGAAGATAGGCTGTGGGAACTTCGGGGAGCTTCGCCTAGGAAAGAATCTGTATACAAA TGAGTACGTGGCTATCAAGCTGGAGCCCATCAAGTCCCGGGCCCCACAGCTGCACCTGGAGTACCGCTTT TACAAGCAGCTCAGCACGACAGGTGAGGCGGACTCGGGTACGGGGCCAGCACTCCTAGGCCAGCAGTGGC TCCGGACACCCAGCATGGATGTTTCCTTCGCAGAGGGCGTCCCTCAGGTCTACTACTTCGGCCCTTGTGG GAAGTACAACGCCATGGTGCTGGAGCTGCTGGGGCCCAGCCTGGAGGACCTTTTCGACCTGTGCGACCGC ACCTTCACGCTGAAGACGGTGCTGATGATCGCCATCCAGCTGATCACGCGCATGGAGTACGTGCACACCA AGAGCCTCATCTACCGTGACGTGAAGCCCGAGAACTTCCTGGTTGGGCGGCCGGGCAGCAAGCGGCAGCA CTCCATCCACATCATCGACTTTGGGCTGGCCAAGGAGTACATCGACCCTGAGACTAAGAAGCACATCCCA TATCGCGAGCACAAGAGCCTGACAGGCACTGCGCGCTACATGAGCATCAACACGCACTTGGGCAAAGAGC AGAGCCGCCGGGATGACCTGGAGGCGCTGGGACACATGTTCATGTACTTCCTGCGCGGCAGTCTGCCCTG GCAGGGGCTCAAGGCAGACACGCTGAAGGAGCGCTACCAGAAGATTGGAGACACCAAGCGTGCCACACCA ATCGAGGTGCTGTGTGAGAGCTTCCCGGAGGAGATGGCCACCTATTTGCGCTATGTGCGGCGCCTAGACT TTTTTGAGAAGCCAGACTACGACTACCTGAGGAAGCTCTTCACTGACCTCTTTGACCGCAGCGGCTACGT GTTTGACTACGAGTATGACTGGGCCGGCAAGCCCCTGCCGACACCCATCGGCACCGTCCACCCTGACGTG CCCTCCCAGCCACCACATCGCGACAAAGCTCAGCTCCACACCAAGAACCAGGCGCTCAACTCCACTAATG GAGAGCTGAACACAGACGACCCCACCGCGGGCCACTCCAACGCCCCCATCGCGGCCCCCGCAGAAGTAGA GGTGGCAGATGAAACAAAGTGCTGCTGCTTCTTCAAGAGGAGAAAGAGAAAATCGCTGCAGCGACATAAG TGAGGGTGGGTGCAGCCGCTCAAGATCCCCAGGCGGGTGTCTAGGAAACTGGCAGTGTCCTTGGTGGTGT GGGGCTCCTTGGCCCACCTGCAGGCACCATCTTTCGGGACTTGGGGTCCCTTCCTTCACACAGGACTTTG GCGGAAATCTCTACACCCGTGTCTCATCATCCCCTTCGAGAGCATTAACTATTTAAACCCAGGAGAGAAG CGCTGTGCCGTCCCTGCTGCGCCCCTGTTAGCTCATAAAGTCCAGCTTGTCCCCTCAATCCAAAGGCCAT TTTCTCGAGGGGGGCAGGCCCGGGGCATGGGTGTCACATGGGGAGGCCTCAGCCACCAAAGGGGAGGCGC GTGCACTTCCACAATGCTGCACCAAAGCCGGGGCGGAGCGCGGGACGGTCACCGCATAGGGACTGGTATG GGCCCCACAGTACCAGGGCGGCCGTGATCCGTGTCCCTGCGTGCAGGGCCCACGGGACAGAATCGCCTTA ACGCCAGGCCTGGGGCAGCAGAGAGGCGGTCCCACAGGAGGGCCAGGCCTTGGTCGTGGGAACTGAGTTC TTGCTTTACGACGTGTACAGAAATGAACTTATGTTTCTCTGGCGCTTCCTTGAAGCCGTAGAGTTTAGGG GCTTTTTTTTAAAAAAAAAAATAAAAGAAAAGAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2648652 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2648652 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17318 same embryo
	EMAGE:17317 same embryo
	EMAGE:17319 same embryo
	EurExpress:euxassay_003717 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source