EMAGE mouse gene expression spatial database

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EMAGE:17321

Cpne2 copine II ( MGI:2387578)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001619. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001619_01	euxassay_001619_02	euxassay_001619_03	euxassay_001619_04

euxassay_001619_05	euxassay_001619_06	euxassay_001619_07	euxassay_001619_08	euxassay_001619_09

euxassay_001619_10	euxassay_001619_11	euxassay_001619_12	euxassay_001619_13	euxassay_001619_14

euxassay_001619_15	euxassay_001619_16	euxassay_001619_17	euxassay_001619_18	euxassay_001619_19

euxassay_001619_20	euxassay_001619_21	euxassay_001619_22	euxassay_001619_23	euxassay_001619_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17321_wholemount_strong.wlz

17321_wholemount_moderate.wlz

17321_wholemount_weak.wlz

17321_wholemount_possible.wlz

17321_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17321_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 18 20

cerebral cortex ventricular layer

moderate

regional

moderate expression: see section 17 19 not examined expression: see section 07

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18 19 20

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09 10 13 14 15

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 moderate expression: see section 05 06 07 08 16 17 18 19 20 21

pons ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 14 15 16 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6958
Entity Detected:	Cpne2, copine II ( MGI:2387578)
Sequence:	sense strand is shown >T6958 CGCAGCCGGGGCCCGAGGAGGGCCAGCCCCAGCCCGGGCGAGCGCACGAGTGGGTACGGCCGCGCGGGTA GCGCCCTGAGCCTGTCCCGCGCCGCCGCCCGGCCGACAGCACAGCGCGCGGGGATGCCCCGGCGGCCCGG AGCACGCTGAGGCTGCGGAAAGCGCGACTGCCAGGAACTGTTGCTGCTGCCGCCACCAACCCCCATGGCC TACATTCCGGATGGGGGTGCTCCTACCGCTGGGGCCATCCCCTTGGGTTCTCAGTGCTGTGTGTGCAAAG TGGAGCTGTCAGTGAGTGGCCAGAACCTGCTGGACCGGGATGTCACCTCCAAGTCTGACCCTTTCTGTGT CCTCTTTATAGAAGACAATGGCAGGTGGATGGAGTTCGACAGAACAGAAACAGCCGTCAACAACCTCAAC CCAGCCTTCTCCAAGAAGTTCGTGCTGGACTACCACTTCGAGGAGGTGCAGAAGCTCAAGTTCGCCCTGT TTGACCAGGACAAGTCCAGTGCCCAGTTGGATGAGCATGATTTCCTGGGCCAGTTCTCCTGCAGCCTGGG CACGATTGTCTCCAGCAAGAAGATCACTAGGCCTCTGCTGCTGATGAATGACAAGCCAGCGGGGAAGGGC GTAATCACGATTGCAGCCCAAGAGCTGTCAGACAACCGAGTCATCACACTGAGCCTGGCTGGCAGGAAAC TGGATAAGAAGGACCTCTTTGGGAAGTCAGACCCTTTTCTTGAGTTTTACAAGCCAGGAGATGATGGCAA ATGGATGCTGGTCCATAGGACCGAGGTGATTAAGTACACCCTGGACCCGGTGTGGAAACCATTCACTGTC CCGTTGGTGTCCTTGTGTGATGGCGACCTGGAGAAGCCCATCCAGGTTATGTGCTACGACTATGACAGTA ATGGAGGCCATGACTTCATCGGCGAGTTCCAGACCTCTGTGCTGCAGATGAGCGAGGCTCGGGATGGTGT CCCGCTGGAGATAGAGTGCATCAACCCCAAGAAGCAGAGGAAGAAGAAAAGCTACAAGAACTCGGGCATC ATTATTCTGAGATCATGCAAGATACACCGAAACTACTCATTCCTGGACTACATTCTGGGAGGCTGCCAGC TCATGTTCACCGTTGGAATAGACTTCACAGCCTCCAATGGGAATCCCCTCGACCCTTCTTCTCTCCATTA TATCAACCCCATGGGCACCAATGAATACTTGTCAGCCATCTGGGCAGTGGGACAGATCATTCAGGACTAC GACAGTGATAAGATGTTCCCTGCTCTGGGATTTGGGGCCCAGTTACCACCAGACTGGAAGGTGTCCCATG AGTTCGCTATCAACTTCAACCCCACTAACCCTTTCTGCTCAGGCGTGGATGGCATCGCCCAGGCGTACTC AGCCTGTCTGCCCCACATTCGCTTCTATGGCCCCACAAACTTCTCCCCGATCGTCAACCATGTGGCCCGG TTTGCAGCCCAGGCCACCCAGCAGCAGACAGCAACGCAGTACTTCATCCTCCTCATCATCACCGACGGGG TCATCAGTGACATGGAGGAGACTCGGCACGCCGTGGTGCAGGCCTCCAAGCTGCCCATGTCCATCATCAT CGTGGGTGTGGGCAATGCTGACTTTGCAGCCATGGAGTTTCTAGACGGGGACAACCGTCGACTGCGCTCA CACACAGGCGAGGAGGCGGCCCGAGATATCGTGCAGTTCGTGCCCTTCCGAGAGTTCCGCAACGCAGCAA AAGAAACGCTGGCTAAAGCGGTGTTGGCGGAGCTGCCTCAGCAAGTAGTGCAGTATTTCAAGCATAAAAA CCTGCCCCCCACCAACTCAGAGCCTGCCTGAGCCCATGCCCAGCCGCAGCATGCCAGCTGAGCCCCCGGC CTCCCCAGGAACATGCACGTTCACCCCGCTTCCTCGTGGGTGGCTTTTTTTTTTTTTTAAAACCAGTCCA CGTTTTCATTTTTTACAACTGGACCTCCACCCACAACTTCCCCAGCCCAGCTGGGCTCCCTTTGTCAGAG TCAATGGATGATGCTTCCAGGACAAATCGGCTTCCTCTCCCCACCCTCCTGCCACTCTAAGTATTGAATG TACTTTGTATAATTTTAGTGGAATTATTGTTATTAAAGAGAATAAAATTTTTACAATCATAACTGGCTTT TTCTAAGTAACTAGCTGCAAGAAGGAATGTATATCCCTTACTGCATACTTTGTATGGTGGTCTGCTGTTT TTATACTTGTGACTGTGTTCTATTTGTAAAACTCAGGGTAATAAAGGAGTTTCAGATGTTGAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985959 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985959 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17323 same embryo
	EMAGE:17324 same embryo
	EMAGE:17322 same embryo
	EurExpress:euxassay_001619 same experiment
	MGI:4824030 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source