EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17368

Gstcd glutathione S-transferase, C-terminal domain containing ( MGI:1914803)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003807. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003807_01	euxassay_003807_02	euxassay_003807_03	euxassay_003807_04

euxassay_003807_05	euxassay_003807_06	euxassay_003807_07	euxassay_003807_08	euxassay_003807_09

euxassay_003807_10	euxassay_003807_11	euxassay_003807_12	euxassay_003807_13	euxassay_003807_14

euxassay_003807_15	euxassay_003807_16	euxassay_003807_17	euxassay_003807_18	euxassay_003807_19

euxassay_003807_20	euxassay_003807_21	euxassay_003807_22	euxassay_003807_23	euxassay_003807_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17368_wholemount_strong.wlz

17368_wholemount_moderate.wlz

17368_wholemount_weak.wlz

17368_wholemount_possible.wlz

17368_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17368_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7751
Entity Detected:	Gstcd, glutathione S-transferase, C-terminal domain containing ( MGI:1914803)
Sequence:	sense strand is shown >T7751 CTGCTCAGGGCCTCGCTGGGAGGCGAGTGCTCCTCCTCAGGCCGCGGCTTCCAGAAGTAGCCTCGGAGTC CCGGCTCGAGCTGGGCGGGAAGGCGCGGTGGCCCGCGTCGTCGCCCGCATGCTCCCGCGCCCTCCCTGCC TGGCCGCGCCCCTGCGACCAGGTGACCCAATGACAACAGAAGATGAAAGGCATTAAGAAAAGTCCTACGG AGGAATCTCTGTACCTGGAGTACTCTCACCAAACAGAAGGCTGCATCTTTCCTCTCCACACGTCTGTAAC TTTGTTTTTACTATCATACTGTGAATGCAAGGTCTTTAACGTTTGCTTAGTCCTCACTGAAGAAAGTACA GATACTTCAGACACTTCGTTCCTAAAAGAGGCACTGCCCCAGGACCTGGAAATACAGGTTATTTCAAAGC AGGCGCTCCCTCCGATTGTCCAGAATTGCTGTTTGCCTGCAGTGGTAGATCAACCTGACGTTTTCTGTAG GGCAGGCCTTGCTGTTGTCCTGAGACACATAATCCAAAAATCCTATGAAGCTGAGCCCTCAAGAAAGGAA ATTTTGGAACTTCTGGGTTTTAAAAAGACTTGTTTGAAAGCTTGTGCTGAAGTTAGTCAGTGGACCAGGC TATGTGAACTCACCATCCCTTTGGCTGTTGAGAATTTTCTCCAAGAGTCCTCTGAGCATCCCCCAACTAT CCCTGAAGAAATCCTAGAGCTCGAGAGGAAGCTCAGTGAGCCTGTCAGAGTACACAATGATGACAAACTC CGAAGACAGAAGCTGAAGCAACAGAAGGCTGCTGGCAGTGAGCCTCCCTCCGGGAAGGGGAAAGCAAAGA GCAAGGCAAGTGCACAGAAGACACCCAAAGACTTGGCTGCTCCCTCCAAGAGTCTGGAACTGAAGGTGGC GTTCTCAAAGCTCACAGTGCAAGAAGATGCAGCTGCTAGCAACAGGGAGCCTTCTCACATCAGGAAAGCA AAAGCCGCCGACCTCCCGCCTCTGGAGCACGTGTTTGCAGAAGGACTGTACTTCACTTTGGCAGACATCG TGCTTTTGCCCTGTATTCACCACTTTTTGGTAATTATCTGCAAGAAATTTTCAGAGAAGCTGGAACAGTT TCCACTACTAACCTCTTGGTATCAGAGGATTCAGGAAGTGCCAAAAGTCAAAACAGCAGCTTCCAAGTGT GGGATCTACTTTCTGTATTTACCAGAATTGCTGAACTCTGCAAGGAAACAGCCTGTGAATTCTGATGAAG TAGCAGCTGTAGATGAGCAAAGTGACCCTTTATTTATAGGAGGACCAAGACCGACTATGACCAAGTTGAT GGAAAAAGGCATTGAAGCCATGTTTTCTCCGCACCCTTGTCCTGCTTGGACCCTTGACTGGAGCAGTCTT CCTGCTGCCGTCAGCCCGAAGGAAGGTAAAATGTCCACTGACCGAGCTCTGAGGAAACAGCAGCAGTTAA ACAACCTTGTCTACCTGGTGCTGAATCAAGCCAAACCTGGGGACAGAATTGTGGATTTCTGCAGTGGCGG GGGCCATGTTGGAATTGTCCTTGCCCACATGCTGCCCTCGTGCCAGGTTACGTTAATAGAGAACAAGGAA TTATCATTAATCCGTGCTAAGAAGAGAAGTGACGAACTTGGTTTAAGCAATATTTGGTTCATTCAAGCAA ATATGGAGTATTTCACAGGAATGTTTAACATTGGGGTGGCCTTGCACGCTTGTGGAGTGGCAACAGACAT GGTGATCGAGCACTGCATCCAAACACGGGCTTCCTTCATCACATGCCCCTGCTGTTACGGGTTCATTCAG AACACATCCAAGTTTAACTTCCCAAAAAGTGAAAAATTCAAGAAAACTTTATCATACAAGGAACACATGC TCCTCTGCAGATTTGCCGATCAGACAGCTGTCCAGCTCCCACCCGAGAGGAGACTCATAGGGAAGCAGTG CATGGGCCTGGTGGATCTGGACCGAGCAGCAGCAGCAGGAGAACACGGCTACTCTGTTCAAGTGATCTCT ATGGAGCCAGAGAGCTGCTCTCCCAAGAACAACATGATTGTGGGTGTCCCCTTGTAGACGGGCACTCAAA CGTGATGCTCTGCCATTGGCTTCGTGAGGAGAGCCCGGGGCCGTCCAGGGAGCCTTTGCTGTGCTCCTGG AAGCAGCACTGAGCTGAGCATGCTGGGCCGGCTGGGAAAGCTGCAGGAAGACCTTGGAAGAGGGATGCTC CGCTGTGTGCGCAGAAGGATGACAGGCTTCCAAGAGGGGATTCCCCGAAGCCTTAGCCGCCAGAGTCGTT CTCAGTGGCCAAGATTCCCTCCACAGATCCATTTCCTCCCACTTCCCTGATGATGTCAGTGGAATATTCA ACCTGGGCTATGACCTCACAGCCTTGACTTTATAATCTTTAGATATTCATACTTGGTGACCAGAGCTAAA CTCAAGGTAGTTGAGGTTTTATAGTTTTAACAATGTACCTTTTCTATAGATTTTTTTAAAGTCTAGAATG GTAAATTTAGTGTTTTTTTTTTAAATCTGACATTGGTTGATCTGCATATCATGTACTATCCTCCTTTTAA ATATAAATATATGTCCCATAAATTAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6311866 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6311866 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17370 same embryo
	EMAGE:17369 same embryo
	EMAGE:17371 same embryo
	EMAGE:17372 same embryo
	EMAGE:17373 same embryo
	EurExpress:euxassay_003807 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source