EMAGE mouse gene expression spatial database

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EMAGE:17487

4930504O13Rik RIKEN cDNA 4930504O13 gene ( MGI:2685370)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011818. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011818_01	euxassay_011818_02	euxassay_011818_03	euxassay_011818_04

euxassay_011818_05	euxassay_011818_06	euxassay_011818_07	euxassay_011818_08	euxassay_011818_09

euxassay_011818_10	euxassay_011818_11	euxassay_011818_12	euxassay_011818_13	euxassay_011818_14

euxassay_011818_15	euxassay_011818_16	euxassay_011818_17	euxassay_011818_18	euxassay_011818_19

euxassay_011818_20	euxassay_011818_21	euxassay_011818_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17487_wholemount_strong.wlz

17487_wholemount_moderate.wlz

17487_wholemount_weak.wlz

17487_wholemount_possible.wlz

17487_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17487_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

spotted

moderate expression: see section 04 05 06 07 09 10 11 13 14 15 16 17 21 22 weak expression: see section 02 03 18 19 20

pituitary gland

moderate

spotted

moderate expression: see section 10

heart ventricle

moderate

spotted

moderate expression: see section 06 07 10 11 13 14 15 weak expression: see section 08 09 16

lung

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 19 weak expression: see section 11 12 13 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9734
Entity Detected:	4930504O13Rik, RIKEN cDNA 4930504O13 gene ( MGI:2685370)
Sequence:	sense strand is shown >T9734 AGGAAGAACCCCGCCTCGAATCCCAGCACCAGAGACGGGACCTAACAGTCTGGACCATGCACCCTGCCAC CCCGAGCAGGCTTCTCTTGGTCTGTAGCCTCGCCTTTATGCCCTTCTCTACAGACCCCTGGGAAATTACG ATGAACGAGATAGAGGAGAAGGAAGTGGATGAATTCAACTCCAGCGGTCTCAAGTGTCCCACATGCTTCA CCGTGAAGGGGAGAGAGTGCAGTCCAGAGCTCAAGTGGTGTTCCACGGACAAGATAAAGTGTGTGGAGCT CTCCGGCATCATAAACACAGGTGTCAACAACGTCCCCATTCAGCTGATGAAATGCATAACCATAGAACAG TGCAAAGACACAGCGACGGCTTATATGGGTTTTACAATCAGTAACAAAAGTGAAATCTGTAAGTCAGCCA TCAGAAACGGGGCCAGAGTCAGACCCCCAACCCCCATCTTCTTTGTTCTCTTCCTGAAGAAACTGCTTCA CTGAGCAGCTGCAGAGGCTCAGCCACCATCACCGAAGCATTAAAGGTGCAGGTTTTGTTTGCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6740346 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6740346 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17486 same embryo
	EMAGE:17488 same embryo
	EurExpress:euxassay_011818 same experiment
	MGI:4822731 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source