EMAGE mouse gene expression spatial database

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EMAGE:17573

Spink3 serine peptidase inhibitor, Kazal type 3 ( MGI:106202)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005920_01	euxassay_005920_02	euxassay_005920_03	euxassay_005920_04	euxassay_005920_05

euxassay_005920_06	euxassay_005920_07	euxassay_005920_08	euxassay_005920_09	euxassay_005920_10

euxassay_005920_11	euxassay_005920_12	euxassay_005920_13	euxassay_005920_14	euxassay_005920_15

euxassay_005920_16	euxassay_005920_17	euxassay_005920_18	euxassay_005920_19	euxassay_005920_20

euxassay_005920_21	euxassay_005920_22	euxassay_005920_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17573_wholemount_strong.wlz

17573_wholemount_moderate.wlz

17573_wholemount_weak.wlz

17573_wholemount_possible.wlz

17573_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17573_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 12 moderate expression: see section 13 14

rectum

moderate

regional

moderate expression: see section 12

midgut

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10046
Entity Detected:	Spink3, serine peptidase inhibitor, Kazal type 3 ( MGI:106202)
Sequence:	sense strand is shown >T10046 AAGCACCCTGTATAGTTCTTCTGGCTTTTGCACCCAGATCTTCGACAATGAAGGTGGCTGTCATCTTTCT TCTCAGTGCTTTGGCCCTGCTGAGTTTAGCAGGTAACACTTTTTCAGCTAAGGTGACTGGAAAAGAGGCT AGTTGCCATGATGCAGTGGCGGGATGTCCCAGAATTTATGATCCTGTGTGTGGGACTGACGGAATTACTT ATGCCAATGAATGTGTTCTGTGCTTTGAAAACAGGAAACGCATAGAGCCTGTCCTCATTCGAAAAGGTGG GCCTTGCTGAAAGTCAAGATTTTGAACTCTGTTATGGCTACCGTAATGTTTGGCAAGTGGGTTCGTTGAA TAAATGCATCTGAACATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6775994 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6775994 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17574 same embryo
	EMAGE:17575 same embryo
	EMAGE:17576 same embryo
	EurExpress:euxassay_005920 same experiment
	MGI:4828412 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source