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EMAGE:17574

Akr1b7 aldo-keto reductase family 1, member B7 ( MGI:101918)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574
"Pseudo-wholemount" of euxassay_005909. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005909_01 euxassay_005909_02 euxassay_005909_03 euxassay_005909_04
EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574
euxassay_005909_05 euxassay_005909_06 euxassay_005909_07 euxassay_005909_08 euxassay_005909_09
EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574
euxassay_005909_10 euxassay_005909_11 euxassay_005909_12 euxassay_005909_13 euxassay_005909_14
EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574 EMAGE:17574
euxassay_005909_15 euxassay_005909_16 euxassay_005909_17 euxassay_005909_18 euxassay_005909_19
EMAGE:17574 EMAGE:17574 EMAGE:17574
euxassay_005909_20 euxassay_005909_21 euxassay_005909_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17574Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17574_wholemount_strong.wlz
17574_wholemount_moderate.wlz
17574_wholemount_weak.wlz
17574_wholemount_possible.wlz
17574_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17574_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal gland
strong strong
regionalstrong expression: see section 05 06 07 08 12 13 14
metanephros
strong strong
spottedstrong expression: see section 05 06 07 14 15 moderate expression: see section 08 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T10045
Entity Detected:Akr1b7, aldo-keto reductase family 1, member B7 ( MGI:101918)
Sequence:sense strand is shown

>T10045
AGAGAACTCTCTGCAGCAACCATGGCCACCTTCGTGGAACTCAGTACCAAAGCCAAGATGCCCCTTGTGG
GCCTGGGCACCTGGAAGTCTTCCCCAGGCCAAGTCAAGGAAGCCGTGAAGGCGGCCATTGATGCTGGGTA
TCGCCACATTGACTGTGCCTATGTGTATCACAATGAGAATGAGGTGGGAGAAGCTATCCAAGAGAAGATC
AAAGAGAATGCTGTGAAGCGGGAGGATCTCTTCATCGTCAGCAAGCTGTGGGCCACATTCTTTGAGAAAA
GCCTGGTGAAGAAAGCCTTCCAGAACACCCTCTCGGATCTGAAGCTGGACTATCTGGACCTGTATCTGGT
CCACTGGCCACAGGGATTTCAGGCTGGGAATGCGTTATTACCCAAAGACAATAAAGGCAAAGTTCTCCTG
AGTAAATCCACATTCTTGGATGCCTGGGAGGCCATGGAGGAACTGGTGGACCAGGGGCTGGTGAAAGCTC
TGGGCATCTCCAACTTCAACCACTTCCAGATTGAAAGGCTCCTGAACAAGCCTGGACTAAAACATAAGCC
AGTGACCAACCAGATTGAGAGCCACCCTTATCTCACCCAGGAAAAACTGATCCAATACTGTCAATCCAAG
GGCATCGCTGTTACAGCCTACAGTCCCCTGGGCTCCCCAGACAGGCCTTATGCCAAGCCAGAAGACCCCG
TAGTAATGGAGATTCCCAAGATCAAAGAGATTGCTGCAAAACACAAGAAAACAGTAGCTCAGGTTCTGAT
TCGGTTCCATGTCCAAAGGAATGTGGTGGTGATCCCCAAGTCTGTGACACCCTCACGCATACAGGAGAAC
CTGCAGGTCTTCGACTTCCAGTTGAGTGAGGAGGACATGGCTGCCATTCTCAGCTTCAACAGGAACTGGA
GGGCCTGTGACCTGTTGGATGCAAGGACTGAAGAGGACTATCCTTTCCACGAGGAATACTGAGGTCCACT
TGCTTGATGAGATCCGTGCATGATAGATTCCTTCGTCTCTCTAAAATCAACCTGGATGTAGGCTACCATC
TGGCAATGCAGGATTTAAATTTTTGTGTTCATCAAGGAGCAAGAAAATGTTCCAGAGAAACGAAGGGCAA
CGGTTCTCACTAGCGAGGGCTGAGCACATGTTTGTCATCAGGAGCATGTAGTATGGTAACACTGAAGATA
CAATGATAGAACAATAAAAAAGCAATAATAGCAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6775941 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6775941 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17575 same embryo
 EMAGE:17576 same embryo
 EMAGE:17573 same embryo
 EurExpress:euxassay_005909 same experiment
 MGI:4823068 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS