EMAGE mouse gene expression spatial database

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EMAGE:17575

Hsd3b3 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3 ( MGI:96235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005913. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005913_01	euxassay_005913_02	euxassay_005913_03	euxassay_005913_04

euxassay_005913_05	euxassay_005913_06	euxassay_005913_07	euxassay_005913_08	euxassay_005913_09

euxassay_005913_10	euxassay_005913_11	euxassay_005913_12	euxassay_005913_13	euxassay_005913_14

euxassay_005913_15	euxassay_005913_16	euxassay_005913_17	euxassay_005913_18	euxassay_005913_19

euxassay_005913_20	euxassay_005913_21	euxassay_005913_22	euxassay_005913_23	euxassay_005913_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17575_wholemount_strong.wlz

17575_wholemount_moderate.wlz

17575_wholemount_weak.wlz

17575_wholemount_possible.wlz

17575_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17575_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 05 06 07 08 13 14 15 16

testis

strong

regional

strong expression: see section 04 05 06 07 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10043
Entity Detected:	Hsd3b3, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3 ( MGI:96235)
Sequence:	sense strand is shown >T10043 AAGTGGACTTTGAAGCTTCCAGCCAATCCTACTGCACACTCTGCTCTCTCCGTCCAGATCAAAAAGGGAG ATCTCAACTGCTCTGGTTTCATGATCTACAACCCTTGAAAACAAAACTGTTGACCATGCCTGGGTGGAGC TGCCTGGTGACTGGAGCAGGAGGGTTTTTGGGCCAGAGGATCATCCAGTTGTTGGTGCAGGAGAAAGATC TTGAGGAGATCAGGGTCCTGGACAAGGTCTTCAAACCTGAAACCAGGGAGCAATTCTTCAGTACCCAGAA CTTATTGGAGGCCTGTATCCAAGCCAGTGTGCCAGCCTTCATCTTCTCCAGCTCAGTTGACGTTGCAGGG CCCAACTCTTACAAGGACATTGTCCTGAATGGCCACGAGGACGAGCATCGTGAAAGCACATGGTCTGACC CATACCCATACAGCAAAAAGATGGCTGAGAAGGCAGTGCTGGCAGCCAATGGGAGCATGCTGAAAAATGG TGGCACTTTGCAAACTTGTGCATTAAGGCCCATGTGCATTTATGGGGAGAGAAGTCAATTCCTTTCTAAC ACAATAATTAAGGCCCTCAAAAATAAGTTTATTCTGAGAGGTGGGGGCAAATCCTCCACAGCCAACCCAG TATATGTGGGCAATGTGGCCTGGGCACACATTCTGGCTGCCAGGGGCCTTCGAAACCCCAAGAAGTCACC AAATATCCAAGGAGAGTTCTACTACATCTCAGATGATACCCCTCACCAAAGTTATGATGATTTAAATTAC ACCCTGAGCAAGGAGTGGGGCTTCTGCCTCAATTCCAGGTGGTACCTTCCTGTGCCCATACTGTACTGGC TTGCCTTCCTGCTGGAAACTGTGAGCTTCCTGCTGAGTCCAATCTACAGATATATACCTCCCTTTAACCG CCACTTGGTCACACTGACAGCTAGTACGTTCACTTTCTCCTACAAGAAAGCTCAGCGAGATCTGGGCTAT GAGCCACTTGTCAGCTGGGAGGAAGCCAAGCAGAAAACCTCAGAGTGGATCGGGACACTAGTGGAGCAGC ACAGGGAGACACTGGACACAAAGTCTCAGTGATGGGAAGATGATGGAGACATGGCCCTGGGTATTACCAG TCCAGTAAGGACAGTAAGGATAAAAGCACAACACAGGTGCTGTTGCCTTCTTTTGACACAGAGGCCAATT TAGTGCCTTAATCAAGTCACCAAAGCCTTGCCAGTCACTGACCCAGCCACAAGCCTTCTTTCTGAAGTAC CTCCCAAAAGACACACAGCCACCTGTGCCCCAGCTCCTGGGCCCAAGCCCCTCAGCACCACTTGTACCTC AGAGCTCTCTCCATTACTTTCTCTCCCCATTCAAAGCATGTATAGCCTTCCGAAAATTCTGCTAGCTTTA TGAAGCTCAATGGAAGAAACAATAAATATTTGTCAATACCTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6768060 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6768060 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17574 same embryo
	EMAGE:17576 same embryo
	EMAGE:17573 same embryo
	EurExpress:euxassay_005913 same experiment
	MGI:4825466 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source