EMAGE mouse gene expression spatial database

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EMAGE:17611

Slc22a20 solute carrier family 22 (organic anion transporter), member 20 ( MGI:2685809)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005954. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005954_01	euxassay_005954_02	euxassay_005954_03	euxassay_005954_04

euxassay_005954_05	euxassay_005954_06	euxassay_005954_07	euxassay_005954_08	euxassay_005954_09

euxassay_005954_10	euxassay_005954_11	euxassay_005954_12	euxassay_005954_13	euxassay_005954_14

euxassay_005954_15	euxassay_005954_16	euxassay_005954_17	euxassay_005954_18	euxassay_005954_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17611_wholemount_strong.wlz

17611_wholemount_moderate.wlz

17611_wholemount_weak.wlz

17611_wholemount_possible.wlz

17611_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17611_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7728
Entity Detected:	Slc22a20, solute carrier family 22 (organic anion transporter), member 20 ( MGI:2685809)
Sequence:	sense strand is shown >T7728 GCCATGGCCTTCACAGACCTGCTGGATGCCCTGGGAGGCGTGGGTCGCTTCCAGCTTGTCTATACAGCCT TGCTGCTGCTGCCCTGTGGGCTGCTGGCTTGCCATACCTTCCTACAGAACTTCACGGCTGCTGCACCCCC TCACCACTGCCAACATCCTGCCAACTACACAGAACCTACCACCAATGTCTCAGGGGTCTGGCTGAGGGCT GCCATACCCCTGAACCAGCATGGGGACCCCGAGCCGTGCCGGCGCTATGTAGAACCTCAGTGGGCCCTTC TGAAACCCAACGCCTCCTCCCATGGGGTGGCCACCGAGGGCTGCAAGGATGGTTGGGTCTACGACCGAAG CATTTTCCCATCTACCATTGTGATGGAGTGGGACCTGGTGTGTGAGGCCCGCACCCTTCGTGACCTGGCT CAGTCCATCTACATGTCCGGGGTGTTGGTGGGAGCCGCCTTGTTTGGTGGTCTTGCTGACAGGTTGGGTC GCAAGGCCCCACTGGTGTGGTCGTACCTGCAGCTGGCAGTTTCGGGGGCTGCCACAGCGTATGTGGGCTC CTTCAGTGCCTACTGCGTCTTCCGCTTCCTGATGGGCATGACCTTCTCCGGCATCATCCTTAACTCCCTC TCCCTGGTTGTAGAATGGATGCCAACCAGAGGCCGTACAGTAGCAGGCATCTTGTTGGGTTTCTCCTTCA CCTTGGGCCAGCTCATCCTGGCTGGCGTGGCATACCTGATCCGGCCCTGGCGGTGGCTGCAGTTTGCTGT GTCTGCTCCTTTTCTGGTCTTTTTCCTCTATTCTTGGTGGCTTCCAGAGTCATCCCGATGGCTCCTCCTT CACGGCAAGGCCCAGCAAGCTGTGCAGAACCTTCAGAAGGTGGCCATGATGAATGGCAGGAAGGCGGAAG GGGAGAGGCTGACTACAGAGGTGGTGAGCTCCTACATCCAGGATGAGTTTGCAAGTGTCCGCACCTCCAA CTCCATCTTGGACCTCTTTAGAACCCCTGCCATCAGAAGGGTCACGTGCTGTCTCATGGGGGTCTGGTTC TCTAATTCTGTGGCTTACTACGGCCTGGCCATGGACCTGCAGAAGTTTGGGCTCAGCATCTACCTGGTAC AGGCTCTGTTTGGGATCATCGATATCCCGGCCATGCTGGTCGCCACCACCACGATGATTTATGTGGGACG ACGGGCCACAGTGTCCTCCTTCCTCATACTAGCTGGGCTCATGGTCATTGCCAACATGTTTATGCCTGAG GATCTGCAGACCTTGCGGACAGTGCAGGCAGCGCTGGGCAAAGGCTGCCTGGCCAGCTCCTTCATCTGCG TGTATCTGTTCACGGGAGAACTCTATCCCACAGAGATCAGGCAGATGGGGATGGGATTTGCTTCTGTCAA TGCCCGCCTCGGAGGCTTGGTGGCACCCCTGATCACCACCCTTGGCGAGATCAGTCCAGTTCTGCCACCT GTATCCTTTGGTGCCACTTCAGTCTTGGCTGGAATGGCTGTCGCCTGCTTCCTGACTGAGACCCGCAATG TGCCACTGGTAGAAACTATTGCTGCAATGGAGAGACGAGTCAAACAAGGCCGTTCCAAAAGAGACACAGA ACAGAAGAGTGAAGAAATTTCTCTTCAGCAGCTGGGGGCTTCACCCCTCAAAGAAACCATTTAAGTTGTC AGGACCCTGACGCTGAGCTCAGCCTGTGCTAGGAGGGAGTGTCCAAGTCCTGCTAGGAGATCCTGGTGCA GAACTCCATGCAAAGCCAGAGGGCATCGTGGACTTTGCTGGGGAGCCACACCCAAGCCCAAGCAGAAAGC TCAGGAAGGGGAAGTATCCTCCAGGGACCCAAGGAGATCATGTTGTCCACTGCAATAAAAGCACTACTGT CCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309674 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309674 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17615 same embryo
	EMAGE:17613 same embryo
	EMAGE:17612 same embryo
	EMAGE:17616 same embryo
	EMAGE:17614 same embryo
	EurExpress:euxassay_005954 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source