EMAGE mouse gene expression spatial database

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EMAGE:17613

Adh7 alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ( MGI:87926)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005947. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005947_01	euxassay_005947_02	euxassay_005947_03	euxassay_005947_04

euxassay_005947_05	euxassay_005947_06	euxassay_005947_07	euxassay_005947_08	euxassay_005947_09

euxassay_005947_10	euxassay_005947_11	euxassay_005947_12	euxassay_005947_13	euxassay_005947_14

euxassay_005947_15	euxassay_005947_16	euxassay_005947_17	euxassay_005947_18	euxassay_005947_19

euxassay_005947_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17613_wholemount_strong.wlz

17613_wholemount_moderate.wlz

17613_wholemount_weak.wlz

17613_wholemount_possible.wlz

17613_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17613_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7742
Entity Detected:	Adh7, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ( MGI:87926)
Sequence:	sense strand is shown >T7742 GTCAGAAGACGTTCCTGTTTGCAGGCCACACACCCAGGAGAGGCAGGATGGGCACCGCTGGAAAAGTTAT TAAGTGCAAAGCGGCTGTCCTATGGGGAGTGAACCAGCCTTTCTCCATTGAGGAAATCGAGGTGGCCCCA CCAAAGGCTAAGGAAGTCCGTGTTAAGATTTTGGCCACAGGAATCTGTCGCACAGATGACCATATAATAA AAGGATCGATGGTGTCTAAGTTTCCAGTGATTGTGGGACATGAAGCAGTTGGGGTTGTGGAGAGTGTTGG AGAAGGGGTCACTACAGTGAGACCAGGTGACAAAGTCATCCCCCTCTTTCTACCACAGTGTAGAGAATGC AACGCCTGCCTGAACCCAGAGGGCAATCTCTGCATTAGGAGCGATCTGACAGGCCGTGGAGTATTGGCTG ATGGCACTACCAGATTCACCTGCAAGGGCAAACCGGTCCAGCACTTCATGAACACCAGCACCTTCACTGA GTACACGGTGCTGGACGAATCCTCCGTAGCGAAGGTTGACGGTGCGGCTCCGCCTGAGAAAGCCTGCTTG ATCGGCTGTGGATTTTCCACGGGTTATGGAGCTGCTGTTAAAACTGCCAAGGTCACCCCTGGCTCCACTT GTGTTGTCTTTGGCCTGGGAGGAGTTGGCCTGTCAGTCATCATGGGCTGTAAAGCAGCTGGTGCCTCCAG GATCATTGGAATCGACATCAACAAAGACAAATTCCAGAAAGCTCTGGCCGTAGGTGCCACAGAGTGTATC AGTCCCAAGGACTCTACCAAGCCCATCAGTGAGGTGCTATCCGACATGACAGGCAACACCATACAGTATA CTTTTGAAGTTATTGGGCGTCTTGAGACCATGGTTGATGCCCTCTCATCTTGCCATATGAACTATGGGAC CAGTGTGGTGGTTGGTGCTCCTCCGTCAGCCAAGATGCTCACCTATGACCCAATGCTGCTCTTCACTGGG CGTACGTGGAAGGGCTGCGTCTTTGGAGGGTGGAAGAGCAGAGATGATGTACCTAAACTGGTGACTGAGT TCCTGGAAAAGAAGTTTGACCTGGACCAGTTGATAACCCACACCTTGCCTTTTAATAACATCAATGAAGG ATTTGAATTGCTGTATTCGGGGAAAAGCATTCGGACTGTCCTGACATTTTGAGTTCCAGAGACCTGGGTG TTCTGAGCTGGGCTACCGTGACCTGAAGCCTTGCTCCTGACAGTGTTCTCTGAGATCACAAGTCTGTCTC AGAAGCACACAGAAGCAGAGCTGGGGAAAGACAGAGAAGCTTTCTAGAGTCTGGCAAACCTTTATCTGCT TAGTTTACCGAGTGCCTAGGCTTCGCAGAGAACTCAGCAGTAACAATAAATGTTCTAATTTGTGTTTTCC AAGGCTATAATTCTATATTTTATAAGCATGTACTTAGGGTTCTATTTGTTGAAAGAGGGATTGTTTTCAG AATTTTACAGTTGTTATGGCTTAAAACAGATGTGGCATGTAGAGATGTAGAAAATGAATTTTTCAAGAAA TGACCATCAAAACCACCAGAGCTCTCATTCTGTTTTTTTTTAACAGAAACACTTTTAAAACATGTTATGA ATTAACTTGAATTAGATTGTGAAATAGCACACATAACTTGATTAGATTAAGAAAGAAATTGGGAAATTTA AGGGAAGGGCACATTTTTCAATGATTAACTTGGAGAATTATCATTATATAACTTTGTGACATGGGAAAAG CACAGCCATGTCTGGAGATGTTAGTCAGTGTTTATCAATATAAGACAAGACATTTTTAGAATAGCAATTA AATATAACCGCATTGTGATATTTACCATACTAAGTTTAATAAAGTCGAATTACAAGCCCTTTAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310424 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310424 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17615 same embryo
	EMAGE:17612 same embryo
	EMAGE:17611 same embryo
	EMAGE:17616 same embryo
	EMAGE:17614 same embryo
	EurExpress:euxassay_005947 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source