EMAGE mouse gene expression spatial database

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EMAGE:17614

Ugt1a10 UDP glycosyltransferase 1 family, polypeptide A10 ( MGI:3580642)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006061. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006061_01	euxassay_006061_02	euxassay_006061_03	euxassay_006061_04

euxassay_006061_05	euxassay_006061_06	euxassay_006061_07	euxassay_006061_08	euxassay_006061_09

euxassay_006061_10	euxassay_006061_11	euxassay_006061_12	euxassay_006061_13	euxassay_006061_14

euxassay_006061_15	euxassay_006061_16	euxassay_006061_17	euxassay_006061_18	euxassay_006061_19

euxassay_006061_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17614_wholemount_strong.wlz

17614_wholemount_moderate.wlz

17614_wholemount_weak.wlz

17614_wholemount_possible.wlz

17614_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17614_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

choroid fissure

moderate

regional

moderate expression: see section 19

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 17 18 20 weak expression: see section 08 09 10 11 12 14 not examined expression: see section 16

4th ventricle lateral recess

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 16 17 18 weak expression: see section 08 09 10 11 12 13 14 15

kidney calyx

moderate

regional

moderate expression: see section 03 04 05 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10062
Entity Detected:	Ugt1a10, UDP glycosyltransferase 1 family, polypeptide A10 ( MGI:3580642)
Sequence:	sense strand is shown >T10062 ACTTTTAAACCAGCTCTATTAGAAAAGCTTACAGTAAAAGCGACTGGATTGCTCTGGACTTTGAATTTAC CATGGTTCCTGCAGCCTTTCCCACCTCTCTTCCTCTGTGTGTGTGTCTGCTCCTGGCCTCTGGCTTGGTC CAGGCAGGCAGGCTGCTGGTGGTGCCCATGGATGGGAGCCACTGGTTCGACATGCAGATGGTTGTGGAGA AACTCATTCAGAGAGGGCATGAGGTTGTGGTAGTCATCCCAGAGGTGAGTTGGCGGCTGGGGAAATCCCT GAATTGTACAGTGAAGACTTACTCGGTTTCTCACACTCTGGAGGACCTGGACAGAGAGTTCAAGTATTTT ACTTACACTCAATGGAAAACTCCAGAACAAAGTATACGTTCTTTCATGACAGGCTCAGCTAGAGGTTTCT TTGAACTAATGTTTTCACACTCTAGGGGTTTGTTTAATGACAAGAAGTTAGTGGAGTACTTGAAGCAGAG ATCTTTTGATGCAGTGTTTCTGGATCCTTTCGATGTATGTGGATTAATTGTTGCCAAGTATCTGTCACTC CCATCAGTGATCTTTGCAAGACTTTCATTTTGCTACTATCTTGAAGAGGGTGCCCAGTGCCCGAGTCTTC TTTCTTATGTTCCTAGACTTTTCTCAAAATACACAGACACCATGACTTTCAAGGAGAGAGTGTGGAACCA TTATATGTACATTGAAGACTATGTATCTTGCCCCTATTTTTTCAAAACTGCTGTTGAAATTGCCTCTGAA GTTCTGCAGACCCCAGTGACTATGACAGACCTCTTTAGCCCAGTGTCTATTTGGTTGTTACGCACTGACT TTGTGTTGGAGTTCCCCAGACCTGTGATGCCCAACATGGTCTTTGTTGGTGGGATGAACTGTCTCCAGGG GAAGCCACTTTCCAAGGAATTTGAAGCCTATGTCAACGCCTCTGGGGAGCATGGCATCGTTGTTTTCTCT TTGGGATCCATGGTCTCAGAGATTCCGGAGAAGAAAGCCATGGAAATTGCTGAGGCTTTGGGCAGAATTC CTCAGACGGTCCTGTGGCGCTACACCGGAACTAGACCATCGAATCTTGCAAAGAACACAATCCTTGTCAA ATGGCTACCCCAAAATGATCTGCTTGGTCATCCAAAGACTCGGGCATTCATCACACACTCAGGCTCCCAT GGTATTTATGAAGGAATATGCAATGGAGTTCCGATGGTGATGATGCCCCTATTTGGCGATCAGATGGACA ATGCCAAGCGCATGGAAACTCGGGGAGCTGGGGTGACCCTGAATGTCCTTGAAATGACTGCTGATGATTT GGAAAATGCCCTTAAAACTGTCATCAACAACAAGAGCTACAAGGAGAACATCATGCGCCTCTCCAGCCTT CACAAGGACCGTCCTATAGAGCCTCTGGACCTGGCTGTGTTCTGGGTGGAATACGTGATGAGGCACAAGG GGGCACCACACCTGCGCCCGGCCGCCCATGACCTCACCTGGTATCAGTACCACTCCTTGGATGTGATTGG CTTCCTCCTGGCCATTGTGTTGACAGTGGTCTTCATTGTCTTTAAATGTTGTGCCTATGGCTGCCGGAAA TGCTTTGGGGGAAAGGGGCGAGTGAAGAAATCACACAAATCCAAGACCCATTGAGAAGTGGGGGGAAGTG AAGGAGAAGTATTAGTTCATTATCTGATCAGTTGAAACTTGGAAACAAGTGTTAAATCCATATTGTTTTT GTTAGGGAAATAATTCACCATACATTATACATTCAGCACATTTAAAAATAATAATAATAACAATCTAATT GCTGGCCACACCCATCAGGGAAGGTTCTAGTATATGTGATGTGCTTTTCCAGTATCTTCAGTCTAGACAA CTCTTGCCATCTGTTGGTAATTTACAGAAAGTCTGGCACTCTGCTTTCAGTGACAGCCCCACAGTTTCCC CTGCTCCCGCCAGCTGACGGCTTTCTCCCCTGGATTCTCAGACTGCCGTGGCCTTCTCCAGTGTTAGTCA TTCTTCATTGTGTTCATGCATTATGGGTGGCAAGACCTTTGGAGCTTTGGGAGAAGAGATGAGGCTGTGA CACTGATGGCCCTGTATTTGAGATAATAATTGTTGCTTGTGCCGGAATTTGATGAAAACCAAAGTATGTT CTAAGGCAAGTACATCTTCTATTGTGTTCCCAAACCAAGAACTTATCAATAAATTCATATAAATTGTATC ACTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30120494 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30120494 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17615 same embryo
	EMAGE:17613 same embryo
	EMAGE:17612 same embryo
	EMAGE:17611 same embryo
	EMAGE:17616 same embryo
	EurExpress:euxassay_006061 same experiment
	MGI:4829081 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source