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EMAGE:18234

Rps6kc1 ribosomal protein S6 kinase polypeptide 1 ( MGI:2443419)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18234 EMAGE:18234 EMAGE:18234 EMAGE:18234 EMAGE:18234
"Pseudo-wholemount" of euxassay_012026. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012026_01 euxassay_012026_02 euxassay_012026_03 euxassay_012026_04
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euxassay_012026_05 euxassay_012026_06 euxassay_012026_07 euxassay_012026_08 euxassay_012026_09
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euxassay_012026_10 euxassay_012026_11 euxassay_012026_12 euxassay_012026_13 euxassay_012026_14
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euxassay_012026_15 euxassay_012026_16 euxassay_012026_17 euxassay_012026_18 euxassay_012026_19
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euxassay_012026_20 euxassay_012026_21 euxassay_012026_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 12 13 15 16 17 moderate expression: see section 09 10 11 14 18 19
vomeronasal organ
strong strong
regionalstrong expression: see section 12 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30681
Entity Detected:Rps6kc1, ribosomal protein S6 kinase polypeptide 1 ( MGI:2443419)
Sequence:sense strand is shown

>T30681
AGCCCAGCTCTCCCAAGTGGCTGGATTCGGGCTCCAGTTCAGAGGATGAGTGCACAGCGGGCTACCTGAC
GCTGTGCAACGAGTATGGGCAAGAAAAGATGGACCTGGTGTCTCTGAGCGAGGAGTCTGTTATGCAGCCT
GAAGGGGACAAGGCTGACACCCAGGCGGTTAGTTCCCCAGCATCCCTCGCCACTGGCAGTGTGAGCCCTA
GCACCCACCTCAGGGTCTTCTCTGGAGGTGAGGATCTAGAGGCGGTTTCTTCTCCTCCAACATCTGAATC
CCTCAGTAGGTCAAAAAACAGCCCTATGGAATTCTTCAGAATAGACAGTAAAGACAGCACAAGTGAGCTC
CTGGGACTGGATTTTGGTGAGAAATTACACAGTCTGAAACCAGAGCCTTTGAAAGCCCTCTTTACCCTTG
AAGATGGAGACAGCCCTTCTCAGAGTCTTGATCCTGGTGAGAGCAAGAGAGAGAGTGAAGCTCAGGACTC
CGTGAGCCGGGGCTCAGATGATTCCGTCCCAGTTATCTCATTTAAAGAGGCGGCTGCAGAGGCTATCAGT
GGGGCTGAGGAGGGAAGGCCTGACCTTCTTGTGAATTTACCTGGGGAACTGCAGCCAACCAAAGAGGCTT
CAGCAATGGACCCTAAGTTTTCACAGGCCAGTGCAGGCAGGCTGGATAGTAAACTGCTGGAAGCCCCGGA
TGTGTTGTGCCTCAGGCTGAGTTCTGAACAGTGCCATGGTCTGGGACCAGAAGGCCCAGAGGAACTGAGC
GATCCCACTGAATTCTGTCCCGGAGGTGTGATCCCAGAGCACGATGCTCAGGCAGATCCCGGGGTGCTAT
TCGAGGCTGCCGTTGATCACAGGAGCTCCCCAGACCAGTTTCTGTTTTCCAGCTTGCGTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6812243), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59804. Forward Primer - name:059804_F_IRAV117_h09_Rps6kc1, sequence:AGCCCAGCTCTCCCAAGT; Reverse Primer - name:059804_R_SP6_IRAV117_h09_Rps6kc1, sequence:GGAACGCAAGCTGGAAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18237 same embryo
 EMAGE:18239 same embryo
 EMAGE:18236 same embryo
 EMAGE:18235 same embryo
 EMAGE:18238 same embryo
 EurExpress:euxassay_012026 same experiment
 MGI:4827811 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS