EMAGE mouse gene expression spatial database

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EMAGE:18576

Pcdhb10 protocadherin beta 10 ( MGI:2136745)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014519. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014519_01	euxassay_014519_02	euxassay_014519_03	euxassay_014519_04

euxassay_014519_05	euxassay_014519_06	euxassay_014519_07	euxassay_014519_08	euxassay_014519_09

euxassay_014519_10	euxassay_014519_11	euxassay_014519_12	euxassay_014519_13	euxassay_014519_14

euxassay_014519_15	euxassay_014519_16	euxassay_014519_17	euxassay_014519_18	euxassay_014519_19

euxassay_014519_20	euxassay_014519_21	euxassay_014519_22	euxassay_014519_23	euxassay_014519_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18576_wholemount_strong.wlz

18576_wholemount_moderate.wlz

18576_wholemount_weak.wlz

18576_wholemount_possible.wlz

18576_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18576_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

radius

moderate

regional

moderate expression: see section 01

humerus

moderate

regional

moderate expression: see section 01 02 03 04 05 22 23 24

tarsus

moderate

regional

moderate expression: see section 05 24

fibula

moderate

regional

moderate expression: see section 02 03

tibia

moderate

regional

moderate expression: see section 02 03

femur

moderate

regional

moderate expression: see section 03 04 05 06 07 08 21 22 23 24

meckel's cartilage

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 17 18 19 20 21 weak expression: see section 13 14 15 16

scapula

moderate

regional

moderate expression: see section 04 05 22

pelvic girdle skeleton

weak

regional

weak expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38571
Entity Detected:	Pcdhb10, protocadherin beta 10 ( MGI:2136745)
Sequence:	sense strand is shown >T38571 TTACTATCTCCGTCACCGACCTGGGCACCCCCAGGCTCACAACCCAGCACACCATAACAGTGCAGGTGTC TGACATCAACGATAATGCCCCCACCTTCACCCGAACCTCCTACACCATGTTCATCCGGGAGAACAACAGC CCTGCCCTGCACATTGGCACCATCAGCGCCACAGACTCAGACGCAGGCTCCAATGCCCACATCACATACT CGCTGCTGCCGCCCCATGATCCGCAGCTGACCCTCACCTCTCTTGTCTCCATCAATGAAGACAATGGGCA GCTGTTCGCACTCAGGGCTCTGGACTATGAGGCCCTGCGGGCCTTCGAGTTTCATGTTCGCGCCACAGAC CGTGGTTCACCCGCGCTTAGCAGCCAGGCTTTGGTACTCGTGGTGGTGCTGGACGACAATGACAATGAGC CCTTCGTGCTCTACCCGTTGCAGAACTCTTCTGCGCCCTGTAAGGAGCTAGTACCCAGAGCTGCGGAGGC AGGTTACTTAGTCACCAAGGTGGTGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150096. Forward Primer - name:150096_F_cDNA_Pcdhb10, sequence:TTACTATCTCCGTCACCGACCT; Reverse Primer - name:150096_N_SP6_cDNA_Pcdhb10, sequence:GCCACCACCTTGGTGACTAAGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18573 same embryo
	EMAGE:18572 same embryo
	EMAGE:18575 same embryo
	EMAGE:18574 same embryo
	EurExpress:euxassay_014519 same experiment
	MGI:4827083 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source