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EMAGE:18816

4930563E18Rik RIKEN cDNA 4930563E18 gene ( MGI:1922620)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816
"Pseudo-wholemount" of euxassay_014080. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014080_01 euxassay_014080_02 euxassay_014080_03 euxassay_014080_04
EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816
euxassay_014080_05 euxassay_014080_06 euxassay_014080_07 euxassay_014080_08 euxassay_014080_09
EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816
euxassay_014080_10 euxassay_014080_11 euxassay_014080_12 euxassay_014080_13 euxassay_014080_14
EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816
euxassay_014080_15 euxassay_014080_16 euxassay_014080_17 euxassay_014080_18 euxassay_014080_19
EMAGE:18816 EMAGE:18816 EMAGE:18816 EMAGE:18816
euxassay_014080_20 euxassay_014080_21 euxassay_014080_22 euxassay_014080_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18816Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18816_wholemount_strong.wlz
18816_wholemount_moderate.wlz
18816_wholemount_weak.wlz
18816_wholemount_possible.wlz
18816_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18816_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40314
Entity Detected:4930563E18Rik, RIKEN cDNA 4930563E18 gene ( MGI:1922620)
Sequence:sense strand is shown

>T40314
CTACCTGAGAACCTTCCAGACGCCAGCTGGACCGCCCGGAGACCATGGACCCCCAGTGTCCCTTCAGAGC
CAGGAGAGAGCGGGCGAGCTAGACGAAAACAGGCCGGGCCGCCACTTGACCTTGAATTAGTAGACTCGGC
CCAGCTCGGGAGGCCGCAGTTTGCAGAGGGCCCGCACTTGCTCCAGAATCCAAAGCAGACGCGGAAGGAG
AGCTTGCACAACGCAGGGAGAGTATCCTCTGTCCCACTGGAGCTTGCGTCTCTGGGGAGATTGTTTAAAG
AAAAAAACATCCCATCAATTGCAAGACACGCTGGACTTTCAATCTCTCGGTGAATCTTTTAAATAGCTAA
GGACTATTAAATGAGAACTAGGTCTAGAACTGGTAATAGGATTCAATCATGTGGCTGATTAATTAACTCA
CAGGCTTTGCCTTGGTCACTGAAGCTAACTGGAGTTAGTCTCCAGAATAACTGTTCACCTCCAGAATAAC
TGTTCACCTTCACTGAGAAAGGCTCCCTGGATATCGCTGTCTCACTTTGTTTTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85666. Forward Primer - name:085666_F_cDNA_4930563E18Rik, sequence:CTACCTGAGAACCTTCCAGACG; Reverse Primer - name:085666_N_SP6_cDNA_4930563E18Rik, sequence:ACAAAACAAAGTGAGACAGCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18815 same embryo
 EMAGE:18818 same embryo
 EMAGE:18814 same embryo
 EMAGE:18817 same embryo
 EurExpress:euxassay_014080 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS