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EMAGE:18831

D230035N22Rik RIKEN cDNA D230035N22 gene ( MGI:2442872)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831
"Pseudo-wholemount" of euxassay_015787. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015787_01 euxassay_015787_02 euxassay_015787_03 euxassay_015787_04
EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831
euxassay_015787_05 euxassay_015787_06 euxassay_015787_07 euxassay_015787_08 euxassay_015787_09
EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831
euxassay_015787_10 euxassay_015787_11 euxassay_015787_12 euxassay_015787_13 euxassay_015787_14
EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831
euxassay_015787_15 euxassay_015787_16 euxassay_015787_17 euxassay_015787_18 euxassay_015787_19
EMAGE:18831 EMAGE:18831 EMAGE:18831 EMAGE:18831
euxassay_015787_20 euxassay_015787_21 euxassay_015787_22 euxassay_015787_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18831Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18831_wholemount_strong.wlz
18831_wholemount_moderate.wlz
18831_wholemount_weak.wlz
18831_wholemount_possible.wlz
18831_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18831_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40449
Entity Detected:D230035N22Rik, RIKEN cDNA D230035N22 gene ( MGI:2442872)
Sequence:sense strand is shown

>T40449
TCCCAAGTGTTGCAAGTATGAGCCACTTTACCTAGCTCTAAGAGAAAAACTTCCTCAATAGGCTAAAATG
GGAGAAACACATCTTTAAGCTTTAGGATGGGATTTGTATAATCTTTTCTGAACTGGTGTTCTTTTAGTTG
GAGTTTAATAATCACCTTAAAAACCAGAGAGTCATTCTATGACCTCCCCCCCACCCCCCAATCAAAAACA
GCCTAGCCAGGAAGCCATCGAAGAGAACTCTTAAAAATTGTGGTAAGGATGTTGAAGTGTAGTCTCCACA
GTTGATGGCTTCTGGCAGGGGGTTGGGTTGGGAAAAACTCAGTTTTCTTTAAGGAGCTGGCCACTGGGAG
TTTGACTATGCTTCAGTGAGTATATGGGCAACACAAATTGGACTTGTGTTTTGTTTTTTGCTTTCCTTTC
TTTGTACCTGTCATTTCCTACTTTGTGGGTTCTTTCATTCTTCCATCCCCTTTTCCACCCTTTCAACCCC
CTAATCCTAGATAAGAGAGAAGCAAAGGATAGAAAGGAAGGGGATGGTGTTATTGTTAGACTACTTCCTG
CTGATTAGGACTGTGAGTTTCCTTGGCAAGTTTGTTCTTCACTGTCAGGATATCTTCTTGTTTCTTCTTT
GCTCACAAAGACCCAACAGCAACCAACTAAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 158937. Forward Primer - name:158937_F_cDNA_Mm.121051, sequence:TCCCAAGTGTTGCAAGTATGAG; Reverse Primer - name:158937_N_SP6_cDNA_Mm.121051, sequence:gttagttggttgctgttgggt. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18836 same embryo
 EMAGE:18834 same embryo
 EMAGE:18832 same embryo
 EMAGE:18835 same embryo
 EMAGE:18833 same embryo
 EurExpress:euxassay_015787 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS