EMAGE mouse gene expression spatial database

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EMAGE:18951

Prrx1 paired related homeobox 1 ( MGI:97712)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014259. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014259_01	euxassay_014259_02	euxassay_014259_03	euxassay_014259_04

euxassay_014259_05	euxassay_014259_06	euxassay_014259_07	euxassay_014259_08	euxassay_014259_09

euxassay_014259_10	euxassay_014259_11	euxassay_014259_12	euxassay_014259_13	euxassay_014259_14

euxassay_014259_15	euxassay_014259_16	euxassay_014259_17	euxassay_014259_18	euxassay_014259_19

euxassay_014259_20	euxassay_014259_21	euxassay_014259_22	euxassay_014259_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18951_wholemount_strong.wlz

18951_wholemount_moderate.wlz

18951_wholemount_weak.wlz

18951_wholemount_possible.wlz

18951_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18951_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 22 23

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 19 weak expression: see section 20

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 03 04 06 19 weak expression: see section 20

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 05

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 05 06 18 weak expression: see section 20

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 06 18

middle ear mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 20 21 22 23

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 21 22

nasal septum

weak

regional

weak expression: see section 09 10 12 13

genital tubercle of male

weak

regional

weak expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38704
Entity Detected:	Prrx1, paired related homeobox 1 ( MGI:97712)
Sequence:	sense strand is shown >T38704 CAGGTTGACATAAACGCACCTAGGATTCCTGGACTGAACCCGATCAGGCTAGTATGCCTTTCTTTAAACC TTGGAGAAACCCAAGTCAAGGCTATTATATATCCTGCCCAGAAGAACTATGTTCACATTCATTTTTCTAT TGAATGACATCTGTGTATCTTTTTAAAGTTAATGCTTGACCCATGGAGGGATTTAAAACTGGTTACAGGG AATTGGGCCCAGATAAAGATGAGAAATGCCACATTTGTGAAACACAAGTGCTTTGACACACTTGATAGGT GCCACTAAATCCCAAAGCCTCATCTAAATGCTCTCTCAGGTCCAAGTAAGTTCTTCAGAGAAACGTCGTA GATGCATTTGGAAGTTTTCTACACACCCCTTGTGAACTTAGCCTGAAGCAGCTCTGATCTCAACGATAGG TATCTGTGTCTGTAAGAATGAGAGAAACCCATGTATGTTCTGGGTCTTCATTGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 160072. Forward Primer - name:160072_F_cDNA_Mm.308890, sequence:CAGGTTGACATAAACGCACCTA; Reverse Primer - name:160072_N_SP6_cDNA_Mm.308890, sequence:GCCAATGAAGACCCAGAACAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18955 same embryo
	EMAGE:18954 same embryo
	EMAGE:18953 same embryo
	EMAGE:18952 same embryo
	EurExpress:euxassay_014259 same experiment
	MGI:4827452 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source