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EMAGE:19000

LOC329820 (LOC329820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000
"Pseudo-wholemount" of euxassay_015792. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015792_01 euxassay_015792_02 euxassay_015792_03 euxassay_015792_04
EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000
euxassay_015792_05 euxassay_015792_06 euxassay_015792_07 euxassay_015792_08 euxassay_015792_09
EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000
euxassay_015792_10 euxassay_015792_11 euxassay_015792_12 euxassay_015792_13 euxassay_015792_14
EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000 EMAGE:19000
euxassay_015792_15 euxassay_015792_16 euxassay_015792_17 euxassay_015792_18 euxassay_015792_19
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euxassay_015792_20 euxassay_015792_21 euxassay_015792_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19000Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19000_wholemount_strong.wlz
19000_wholemount_moderate.wlz
19000_wholemount_weak.wlz
19000_wholemount_possible.wlz
19000_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19000_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 10 11 12
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11
dorsal root ganglion
weak weak
single cellweak expression: see section 04 05 06 07 08 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40473
Entity Detected:LOC329820, (LOC329820)
Sequence:sense strand is shown

>T40473
CTGTCTCTCCAGCCCTCATAGTTCTTTTTGTTGTTTAACACTGCTTCTTGTAATAGAGTCTTACTACATG
GTGTTAGTTGTCTTGAAGCTTTCCATGTAGACCATGCTAGCTTCAAAATTGCAGTGATCGACCTGCCTCT
GCTTCTGGAGGGCTAGAATTACAATCACAGCACACCTTTACAAAATGTTCTCTTTCTCTCTATACTTCTC
TGATCCCCACTCTTCAAAGTGGGGTAGCTTTGATGAACCACTTACTGTGTTGCATACAGTCAGGTGCCAT
ATAGTTGAGTCTTATTCACAATCTGGTAGCACTTTAATAGTAGAAACTGTATTATGTCCAGTCTACTTTC
TATAATGTCACTTGGCAAGTGACAGTGTACTTAGTAGTCAATATGTATCCATTGAAATGAATGCAATCCA
GTTTGTATTTGACAGTAGCCTGTCGTCCTTGTTCTCATTATAAACAGTTTAGACTGTAGAGAGACCATAT
TTGCTCTCTAGCCTAGGTAAACGGTACCCTGCTTCTGCAGTTCTTCTTGCAGCTGATCCGCCTGACCAAG
TCGCTTTGCGGGGGGTCGGCTCCTGCAGCCTCCAGTTAGCATCTCAAAGTGACTCAGTGGAAGGCTAGGT
AAAGCCGCCACAGGAAATTGCCCTCCTCTAAGTGGAGAGGGACTTATGTCGGCCCTTTGTTCTTAGTACT
TGTGTGACTCTCAGAAACTGTGTTCACATTGGGGGGGGGGGGGGGACGACACTCACTGCAGGTCTGCACA
TAAATTCAGTCATAAATTGAATAAGAGGTTATAACAGAGAATGCCTACGTGCCTAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 159837. Forward Primer - name:159837_F_cDNA_Mm.274484, sequence:CTGTCTCTCCAGCCCTCATAGT; Reverse Primer - name:159837_N_SP6_cDNA_Mm.274484, sequence:ATAGGCACGTAGGCATTCTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19005 same embryo
 EMAGE:19004 same embryo
 EMAGE:19003 same embryo
 EMAGE:19001 same embryo
 EMAGE:19002 same embryo
 EurExpress:euxassay_015792 same experiment
 MGI:4822828 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS