EMAGE mouse gene expression spatial database

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EMAGE:19169

Snapc3 small nuclear RNA activating complex, polypeptide 3 ( MGI:1916338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009132. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009132_01	euxassay_009132_02	euxassay_009132_03	euxassay_009132_04

euxassay_009132_05	euxassay_009132_06	euxassay_009132_07	euxassay_009132_08	euxassay_009132_09

euxassay_009132_10	euxassay_009132_11	euxassay_009132_12	euxassay_009132_13	euxassay_009132_14

euxassay_009132_15	euxassay_009132_16	euxassay_009132_17	euxassay_009132_18	euxassay_009132_19

euxassay_009132_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19169_wholemount_strong.wlz

19169_wholemount_moderate.wlz

19169_wholemount_weak.wlz

19169_wholemount_possible.wlz

19169_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19169_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 07 15 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 16

trigeminal v nerve

strong

regional

strong expression: see section 10 16

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 08 13

cervical ganglion

strong

regional

strong expression: see section 07 08 15

thoracic ganglion

strong

regional

strong expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

neural retina

strong

regional

strong expression: see section 02 03 04 05

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3457
Entity Detected:	Snapc3, small nuclear RNA activating complex, polypeptide 3 ( MGI:1916338)
Sequence:	sense strand is shown >T3457 CTGACATGGCGGAGGACNGGCAGGGCGGCGGTGCGGGTGGCCCACAGCACCCAGTCCCCAGTGCGAGCCA CAGCAGCTTTCCGGAATACGAGCTTCCCGAGTTGCACACGCGCGTGTTCCATGTGGGCTCCTTTGGGGAG CTATGGCGTGGCCGGCTAGGGGCCCAGGATTTGTCGCTGAGCGAACCGCAGGCAGCCGAGCAGCCGACCG ACGGAGGGGCATCCAACGACGGCTTTGAGGATGCTGCAGTAGCCAGCGATCTGGGCTGCAGTCTGGAGGC AGCAGCAGAGCTGAGGGTCGTGTGCGGCCTTGATAAACTGAGATGCCTCGGGGAAGATGAAGACCCGGAA GTCATCCCAGAGAACACTGACCTAGTGACTTTATGTGTGAGGAAGGGGCTTTTGGACTATCGGGAAGAAA ACATCACAATAGATCGCGCCTGTAGACAAGAAATATTTGCTTATGAAATGGAATCTCATGCACTAGGGAA GAAACCTGAAAACCCAGCAGACATGATTGAAGAAGGAGAGTGTATCCTATCT
Notes:	The probe template was PCR amplified from IMAGE:329883 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:329883 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19170 same embryo
	EMAGE:19171 same embryo
	EMAGE:19168 same embryo
	EMAGE:19167 same embryo
	EurExpress:euxassay_009132 same experiment
	MGI:4828345 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source