EMAGE mouse gene expression spatial database

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EMAGE:19241

Pih1d2 PIH1 domain containing 2 ( MGI:1919864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006264. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006264_01	euxassay_006264_02	euxassay_006264_03	euxassay_006264_04

euxassay_006264_05	euxassay_006264_06	euxassay_006264_07	euxassay_006264_08	euxassay_006264_09

euxassay_006264_10	euxassay_006264_11	euxassay_006264_12	euxassay_006264_13	euxassay_006264_14

euxassay_006264_15	euxassay_006264_16	euxassay_006264_17	euxassay_006264_18	euxassay_006264_19

euxassay_006264_20	euxassay_006264_21	euxassay_006264_22	euxassay_006264_23	euxassay_006264_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19241_wholemount_strong.wlz

19241_wholemount_moderate.wlz

19241_wholemount_weak.wlz

19241_wholemount_possible.wlz

19241_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19241_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 16 17 18 19 weak expression: see section 15

4th ventricle

moderate

regional

moderate expression: see section 03 04 05 06 08 09 10 13 14 16 17 18 19 weak expression: see section 11 12 15 20 21

4th ventricle lateral recess

moderate

regional

moderate expression: see section 07

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 weak expression: see section 11 12 13 14 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5466
Entity Detected:	Pih1d2, PIH1 domain containing 2 ( MGI:1919864)
Sequence:	sense strand is shown >T5466 CGGACGCGTGGGCTCGCCGGTCGCCGTGGAAGCTGACGGGTGCAGGAAGGAAGGCGGCGCTCTCCCGAGA GACAAGCGGGACGGCCTGCGGAGGAGCCAGTCCGGACCCGGAGAAGAGAACCGGAACTGGATATAGAGAC ACAGCTCTACTTTGCGCTCTCCGGACCCAGTTAGATGGTGTACCGGGACTGGTTACCATGGTAACCGGAG TCGCAGTTTGTCGGCAAACTGAGAGAAGACGTGCGCGTTTCCCCCACGTTTGGATTAAGTTCCAAAGGCC TGTCTCATCACCATCATGACGGTGTCCTCAAAGGGTCTTCTTACCCACATTTCCCAGTTCTGGAATATGC TCGATGATTTGGCTGAAAATGACCCCGAGCGCTACCGGAACTTCATTCAGCAGGAGTTGAAAGATGGCAA ACAGCTCTGTGTCAACCCAGAACCTCAACTCTGCATACAGACCAAGATCCTAAAACCAAACGAAAAAGTA CTTTTTATCAACCTATGTCAATGGGAAAGGATTCCCGCTCCGCAGTCAGCCACTCGTCCTGTGCCTGTAA GTGTTGGCAGACCAGAAGACTCCGCTGAGGCATCAGATGCTTATACCATCATTGATGTTGCCTACAATCC TGGTGTTCTGCAAGCGGCAGAAAAAGACCAAGGGATCAAAGATCAGTTAATAAGAATGGCCATGCTGTGC ATCGAGGAGCGACTCCAGTTCACACTTGCACATTCCTACCATTTAACTAGTTTTAGACTGAAAGGAAGCA TCCAGAGAATGAAAGAAAGTCTGATGGGCATTAAAACTGACTTCCCAGACCTAAAAGCGATGACGAGAAC AGAAAATACTCTTGCGAGGATCAGAAGCAGCACTGTGAGTGAACCTAATCACCTTCCTGAGGTGCTATTG ACAAAAAAGCAAGCGTCGGCCAAAGGACGATGTCTTATAGAAGAGATTTCTAGTTCGGAAATCCAGGTGG AGGTTAAGAAACCTGCGTACGAATTAAAGGTTGTAAAGGATCGGAACGAGAAACCTCTGAAAATTGAGCT GAAAGTCGAGTTACCCGGGATTAAGTCAGTCTCTCTCTGTGAACTTAGTGTTTCTGAGGTTGATATATTG ATTGAGGTCTCTGAGATGTACCGATTATGCCTGAATCTTCCAGAATCGATTAATACAGAAATGACTACAG CAAAATTTGTCAAAAATAAATCTGCATTAATCATTACAATGCCCCTGGCATAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5373181 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5373181 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19240 same embryo
	EMAGE:19242 same embryo
	EMAGE:19243 same embryo
	EurExpress:euxassay_006264 same experiment
	MGI:4827205 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source