EMAGE mouse gene expression spatial database

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EMAGE:19267

Mettl6 methyltransferase like 6 ( MGI:1914261)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002093. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002093_01	euxassay_002093_02	euxassay_002093_03	euxassay_002093_04

euxassay_002093_05	euxassay_002093_06	euxassay_002093_07	euxassay_002093_08	euxassay_002093_09

euxassay_002093_10	euxassay_002093_11	euxassay_002093_12	euxassay_002093_13	euxassay_002093_14

euxassay_002093_15	euxassay_002093_16	euxassay_002093_17	euxassay_002093_18	euxassay_002093_19

euxassay_002093_20	euxassay_002093_21	euxassay_002093_22	euxassay_002093_23	euxassay_002093_24

euxassay_002093_25	euxassay_002093_26	euxassay_002093_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19267_wholemount_strong.wlz

19267_wholemount_moderate.wlz

19267_wholemount_weak.wlz

19267_wholemount_possible.wlz

19267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5489
Entity Detected:	Mettl6, methyltransferase like 6 ( MGI:1914261)
Sequence:	sense strand is shown >T5489 CCACGCGTCCGCTGGTCTAAGGAGGAGCCCAGGTCAGCTAGAAGATTCTGCTGCCGACGCCTGGGCTTGA CTGACGGCATCGTATCCTGTTGGAGCTGGAAAGTCACCTGGGGAAACTGCATCAGAGAAACTCCAGTGTC TACAATCTCAGCATCGTTTGTTCAGATGGTTGAAGAACAGAGGACCTATAGCAGCATTGAATGTGTTTCC GGCAACTACAATGGCTTCTTTCCAAAGGAAAGGACTGCAAGCAAGGATTCTTAGCACTGAAGAAGAGGAG AAACTGAAAAGAGACCAAGCTTTGGTGTCTGCTTTTAAACAGCAAAAACTGGAAAAAGAGGCTCAGAAGA ACTGGGACCTTTTTTATAAAAGAAACAGTACCAATTTTTTCAAGGACAGACACTGGACCACCAGAGAGTT TGAGGAGTTGAGATCATGTAGGGAGTATGAAGGTCAAAAATTGACTTTGTTGGAGGCTGGCTGTGGGGTT GGGAACTGTTTATTCCCACTTTTAGAAGAAGACTTGAATCTCTTTGCCTATGCCTGTGATTTTTCTCCAA GGGCAGTTGACTACGTGAAGCAACATCCTTTGTACAATGCAGAGAGATGCAAAGTGTTCCAGTGTGACCT CACTAGAGACGACCTGCTTGACCACGTCCCACCAGAGTCTGTGGATGCCGTTACATTGATCTTTGTGCTC TCAGCTGTGCACCCTGAGAAGATGCGCCTTGTCTTACTAAACGTGTACAAGGTATTAAAACCAGGCAGAA GTGTCTTATTCCGTGACTACGGGCTGAATGATCACGCCATGCTTAGATTTAAAGCTGGAAGCAAACTTGG AGAAAATTTTTATGTCAGGCAAGATGGAACCAGATCGTATTTTTTTACTGATGAATTCCTGGCGCAGCTC TTTGTGGATGCAGGTTATGAAGAAGTGGTGAACGAGTATGTGTTTCGAGAGACAGTGAATAAAAAAGAGG GCCTGTGTGTGCCTCGAGTTTTCCTTCAGAGCAAGTTCCGGAAGCCTCCGAAGGACCCAGCCCCTACCAG TGACTCTGCTTCACTTTGAAGAAAAGGATGTTAAACTATTTTGTATTTTTTTTATATTCTGAATTTTTGA AAATTGCATGTGTTATTTTTGTACTGAGAACAAGTGAAGACTGGGGAGTTGGCTCAGCAGGTAAAGGTGC TAGCTGTGCAAGCTTAGCAACCTGGGTGGATCAGCAGTCTGTGTTGAGCAGGAGAGATGGCTCAGTGATC AAGAGTGTTGGCTGCTCTTGGGCAGGGCCTGGGTTCGGTTTCCAGCACCCACATGGCAGCTCACTTCCAT CAGTAATTCCAGTCCCAGGGTATTGAGTGCCCTCTTCTGACCTCTGCAAGTGGTGCACAGACATACATGC AGGCAAACACCCAAATTACAAAAGAAAAAAATCCAGGTTTGGCAATTTGAGTTTATCCTAGTACTCCTAC CTCAGTACTCCTACCTCAAGATGAGAAGCAGAGACGGAAGAACCATCTGGAAGCCTCAGGAGCAGCCAGC CTGGAGTACACGGTGTGGGAGACCTAAGCCTGGAGTACGCGGTGTGGGAGACCTAGGAGAGAGGCTTCAG AGAGGTGGAGAGTGAGAGCCAATGAGAATTGTTTTTCTGACCTCACATGTGCACAAATAAGTAAATAAAG TGGATTTGTTTGCACACTGTAGAAAAACAGAATTGTAAGTCCTGAAAGGAAAGTCTTGTGGCTTAGTTGT GTTGTATGATTATCCATCTCCACAAAGGGAAGCATGGGAGCCGACAAGAGATACATGAACAGGTCGCAGG TCAAGCATCTGCTGATGATACATTTAAGACAGTTCTTTGGTGTGCATACAGACTGACCACTTATTAAAGA CTAAAGCAGACGTGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5400506 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5400506 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826171 same experiment
	EurExpress:euxassay_002093 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source