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EMAGE:19323

Tnk2 tyrosine kinase, non-receptor, 2 ( MGI:1858308)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323
"Pseudo-wholemount" of euxassay_002030. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002030_01 euxassay_002030_02 euxassay_002030_03 euxassay_002030_04
EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323
euxassay_002030_05 euxassay_002030_06 euxassay_002030_07 euxassay_002030_08 euxassay_002030_09
EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323
euxassay_002030_10 euxassay_002030_11 euxassay_002030_12 euxassay_002030_13 euxassay_002030_14
EMAGE:19323 EMAGE:19323 EMAGE:19323 EMAGE:19323
euxassay_002030_15 euxassay_002030_16 euxassay_002030_17 euxassay_002030_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19323Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19323_wholemount_strong.wlz
19323_wholemount_moderate.wlz
19323_wholemount_weak.wlz
19323_wholemount_possible.wlz
19323_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19323_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 14 15 16
brain
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 18
spinal cord
moderate moderate
homogeneousmoderate expression: see section 09 10 11 12 13
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4571
Entity Detected:Tnk2, tyrosine kinase, non-receptor, 2 ( MGI:1858308)
Sequence:sense strand is shown

>T4571
CCCACGCGTCCGGCCCCAGGCTCCTGCTGGCTTCAGCTGCTAATACTGCCACAGCGGATGGGTCAACTTC
TAGACTGCCTTCTTCTGAGGTGCCAGCTTTTACTCGGTCCCTAGAACTGCTCAGGAGGTGGGAGGGCTGA
GAACTACTGGTGGCGTGGGCAGAATACGCGGACCCTGTGTGTAGGACCCTTCCCTCGAAATGTCGTGACC
TCCGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGACATG
GTGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCCAT
GGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGAAA
AAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCTGA
GGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCAGA
CCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGCCC
TGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAGCC
CCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCCCC
GCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGCTA
GTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGCGC
AGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCAGT
GCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCCAG
CTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCCTC
GGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCTGG
ACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCCCC
CTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCACCA
CTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCAGG
CCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTGAG
CGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGCCC
TGCCCGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGACC
TATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGCCA
CCATCCCTGAGGGCCGCGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCGGC
CAGCAGACAAGGTCCAGATGCTGCAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCCCT
GCAGAGCCACAGCTGGAGTGTTCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTTGGGCTGGGT
CTTCGGCCACGGGTGGAGTGCCACAAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTC
ACCTTCTGGGCTCCTGTGGCCCTGCTCATCACAAGCGCTGATGGTTGACTGGAGAGCAGTGGTCTGCCCT
AAAAGAACCTCTTGAATCTGTCTGTGGGACAAGGGTGGGGGAGCTGCCCTGCCTCAGGCCCGAAGGACCC
ACAACTGCCTGCGGCTCCCAGGGGACAGTGAGACCCAGGCAGCAGGAGGCTGGGAGCCTCACCTTGCCCA
CCTCGCTCACCCACCCAGCGCTGTTCTGCACACCTTGGTTCAGCCCTCAGTGCCCCAAGAGGTGGGACGT
TCCCAGCCTTGTGAAGGCAAGACAGGAAGCTGCCTGGCAGGCTCTGTGTGGCCTGCCTCTGGCTCCAGAT
CAAGGCTGGGCTGGTGGCTAGAGTGTGCCCCAGGCTTTCCTCTGGGGGATTAGACTGACAGGAAGCCAGG
CTTGACATGGACAAGAAGGATGTGTTGGCCACACAGCAGGGCGAGCCAGCAACCAACAGGCCCTCCTCAG
GGCTAGGCCCCTTCCCAGGGAACCCCTGTAGGAGTGTGGAGTGTTGGACAGAATGTGACCTGTAGCTTCA
CTGTGAACTTGTGGGCTTGGCTGGTCTTAGGAACTTGTACCTATAAACAGCCTGAAGTGGCTCAGAAACA
GAAAAGGTGCCAGGATCTTCTCTGGCAGGGACCAGGGCCCAAGGCCCCGGATGTGGAAGGAGACCCCAGT
GGCACCAGTACTTTCCTGTTTGACCTGTTCCTCCCCTGTTGCTATCTGATGTGTTCCCATCAGCCTCTGT
TGCCAAAGTCGTGGCCCCAGCTATGGATATCTGCTTCTTCCAGAGAAATAAAGCTTGTTTCTATTTTATG
TTAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4984168 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984168 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19324 same embryo
 EMAGE:19321 same embryo
 EMAGE:19322 same embryo
 EurExpress:euxassay_002030 same experiment
 MGI:4828851 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS