EMAGE mouse gene expression spatial database

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EMAGE:19433

Actr8 ARP8 actin-related protein 8 homolog (S. cerevisiae) ( MGI:1860775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004822. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004822_01	euxassay_004822_02	euxassay_004822_03	euxassay_004822_04

euxassay_004822_05	euxassay_004822_06	euxassay_004822_07	euxassay_004822_08	euxassay_004822_09

euxassay_004822_10	euxassay_004822_11	euxassay_004822_12	euxassay_004822_13	euxassay_004822_14

euxassay_004822_15	euxassay_004822_16	euxassay_004822_17	euxassay_004822_18	euxassay_004822_19

euxassay_004822_20	euxassay_004822_21	euxassay_004822_22	euxassay_004822_23	euxassay_004822_24

euxassay_004822_25	euxassay_004822_26	euxassay_004822_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19433_wholemount_strong.wlz

19433_wholemount_moderate.wlz

19433_wholemount_weak.wlz

19433_wholemount_possible.wlz

19433_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19433_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6526
Entity Detected:	Actr8, ARP8 actin-related protein 8 homolog (S. cerevisiae) ( MGI:1860775)
Sequence:	sense strand is shown >T6526 AGCCCCGGGGCTGGTGGTGGTCCACGGTGGCAGAGATGACCCAAGCCGAGAAGGGGGACGCGGAGAACGG GAAGGAGAAGGGCGGGGAGAAGGAGAAGGAGCAGCGCGGCGTGAAGCGGCCCATCGTACCCGCGTTGGTC CCCGAGTCGCTGCAGGAGCAAATCCAGAGCAACTTCATTGTTGTCATACACCCAGGTTCAACAACTCTAA GACTTGGCCGAGCCACAGATACACTTCCGGTCAGTGTTCCTCACGTCATTGCACGAAGACACAAACAACA AGGGCAGCCCCTGTACAAGGACAACTGGCTCCTAAGGGAAGGACTAAATAAACCTGAAAGTAATGAACAA AGACAAAATGGCCTTAAAATGGTGGACCAGGCAATATGGTCTAAAAAGATGTCAAACGGTACAAGGCGGA TCCCTGTGTCACCAGAACAGACACGCTCCTACAACAAGCAGATGCGACCTGCCATTCTAGATCACTGTTC TGGAAACAAATGGACAAACACATCTCAGCAGCCTGAGTATTTGGTAGGAGAAGAGGCCTTGTATGTTAAC CCCCTGGACTGTTACAACATTCACTGGCCTATCAGAAGGGGTCAGCTGAACATCCACCCAGGCCCCGGAG GCTCCCTCACAGCTGTTCTGGCAGACATTGAAGTGATCTGGTCTCATGCAATACAAAAGTATTTGGAAAT CCCACTGAAAGATTTAAAGTATTACAGGTGCATCTTGTTAATTCCAGATATCTATAACAAGCAGCATGTG AAAGAGCTGGTCCATATGATACTGATGAAGATGGGCTTCGCAGGGATTGTGGTCCATCAGGAGTCTGTGT GCGCTACGTTTGGGAGCGGCTTGAGCAGCACCTGTGTTGTAGATGTTGGGGACCAGAAAACAAGTGTGTG CTGTGTAGAGGATGGGGTGTCTCATCGGAACACACGGCTTTGTCTGGCCTATGGGGGCTCTGACGTGTCG AGGTGTTTTTACTGGCTCATGCAGCGAGCGGGATTCCCTTACAGAGAGTGTCAGCTGACAAATAAAATGG ATTGCCTCCTCTTGCAGCACCTTAAAGAAACCTTTTGTCATTTAGATCAGGATATCTCCGGACTTCAGGA CCATGAGTTTCAGATTCGGCATCCGGATTCCCCTGCCCTGCTTTACCAGTTTCGATTAGGAGATGAAAAG CTCCAGGCTCCAATGGCGCTTTTTTACCCAGCAACCTTTGGGATCGTGGGACAGAAGATGACCACTCTGC AGCACAGGTCCCAGGGAGACCCTGAGGATCCCCATGATGAGCACTACCTGCTGGCCACCCAGAGCAAGCA AGAACAGTCTGCAAAAGCCACTGCTGACCGCAAGTCTGCATCCAAACCCATTGGATTTGAAGGGGATCTG CGTGGCCAGTCCTCTGATCTTCCAGAAAGACTCCATTCCCAGGAGGTGGACCTGGCTTCTTCTCAGGGAG ACTGTTTGATGGCGGGCAATGAGTCCGAGGAAGCCCTCACTGCACTGATGTCCAGGAAAACTGCCATCTC GCTGTTTGAGGGGAAAGCCTTGGGCCTTGATAAGGCCATCCTGCACAGCGTAGACTGTTGCTCATCCGAT GATACCAAAAAGAAGATGTATAGCTCTATCCTGGTTGTGGGAGGCGGTTTGATGTTTCATAAGGCTCAAG AGTTTCTCCAGCACAGAATTCTCAACAAAATGCCACCTTCATTCAGGCGAATTATTGAAAACGTGGATGT GATCACAAGGCCCAAGGACATGGACCCCCGGCTGATCGCATGGAAAGGAGGTGCAGTACTGGCGTGTTTG GACACCACTCAGGAACTGTGGATTTATCAGCGAGAGTGGCAACGCTTTGGTGTCCGAATGTTACGGGAAC GAGCTGCCTTTGTGTGGTGAAAACCACAACCGAGTCTCAGGACATAAAAGACTTAAAGAGTGTATGTATT GTAAGTTATTGACCACGAAGCTTAATTTTCCTAGAATTAAATTAATGCAATTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590945 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590945 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19432 same embryo
	EMAGE:19431 same embryo
	EurExpress:euxassay_004822 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source