EMAGE mouse gene expression spatial database

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EMAGE:19532

Dido1 death inducer-obliterator 1 ( MGI:1344352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004955. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004955_01	euxassay_004955_02	euxassay_004955_03	euxassay_004955_04

euxassay_004955_05	euxassay_004955_06	euxassay_004955_07	euxassay_004955_08	euxassay_004955_09

euxassay_004955_10	euxassay_004955_11	euxassay_004955_12	euxassay_004955_13	euxassay_004955_14

euxassay_004955_15	euxassay_004955_16	euxassay_004955_17	euxassay_004955_18	euxassay_004955_19

euxassay_004955_20	euxassay_004955_21	euxassay_004955_22	euxassay_004955_23	euxassay_004955_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19532_wholemount_strong.wlz

19532_wholemount_moderate.wlz

19532_wholemount_weak.wlz

19532_wholemount_possible.wlz

19532_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19532_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6913
Entity Detected:	Dido1, death inducer-obliterator 1 ( MGI:1344352)
Sequence:	sense strand is shown >T6913 CTATTGAGTAAATCCATGAAGGATGACAGGAGGGTGGAGGACAGGACAATGGCAGCAGTTACCATCCCGA AGAAAGCACTTCCTTCAGCCTCTTTGGTGGGCAGACAGACTTCACCTAGGAATCTTGTTCCAAAGAAGCT TCCTCCTTACTCTAATATGGCAGGAGCCAAACCAGCCATTAAGAAACTGCCTTCAGGCTTCAAGGGTACC ATCCCCAAGAGGCCATGGCCCTCAGCCACCCTGTCAGGCACTTCTGCCAGGCAGGCAGGACCAACACCTA TGACAGCTGCTTCCAAAAAGTTGCCTGGCTCTGCTGCTGTGGTGGGAGTTACCAGGAAGCCAATGTCTGC CAATGTTCCTGCTGCTTCTCCAGCCCCAGGACGGCTTGGTCCTGTGAGCCCAGCTCCATCACAGCCCAAT TCACAAATTCGACAAAATATAAGGCGCTCCTTGAAAGAGATTTTGTGGAAAAGAGTCAATGACAGCGATG ACTTAATAATGACAGAAAATGAAGTAGGAAAAATTGCCCTCCACATTGAGAAGGAGATGTTTAACTTGTT CCAGGTTACTGATAATCGCTATAAGAGCAAATATCGCAGCATCATGTTCAACCTTAAGGATCCTAAGAAT CAGGGGCTCTTCCATCGTGTTCTTCGAGAAGAAATCTCCTTGGCAAAACTTGTCAGAATGAAGCCTGAAG AACTTGTATCTAAAGAGCTTTCTATGTGGACAGAGAAGCCCACAAAATCTGTGATAGAATCCAGGACTAA GTTGCTTAATGAAAGCAAGAAGAACACTACTAAACCAGAAACCATTCCTGACATGGAAGATTCTCCACCG GTATCAGATTCAGAAGAACAACAAGAGTCAGTGCGAGCCGCCCCTGAGAAGAGCGCAGCACCTCTCCTGG ACGTCTTCAGCAGCATGCTGAAGGACACCACAAGCCAGCACCGTGCCCATCTTTTTGATTTAAACTGTAA AATCTGTACAGGTCAGGTTCCATCCTCGGAGGATGAACCAGCTCCTAAGAAGCAAAAGCTTTCAGCTTCT TCTAAGAAGGAAGACTTTAAGCCCAGGCATGACAGCTCTCCACCTAATGCAGTTCCTAACACTGCTGATG AAGGGATTGCAGACACGCTGCCTGAAAATGCCTCTGAGCCAGACCCGGAGAGTACATCTAGTCTTAACCA GGAGAGAAAGTGTTTCCCTGAGTCTCCAGGCGATAGCCATCCTGAGCCCTCGTCTCTGGGTGGCCTTTCT CCCTCTTCTGCCTCTGGTGGGAGTGGGGTGGTCACCACAGTCACCATGTCTGGTCGAGACCCCAGAACTG CCCTGAGTGGGTCGTGCACAGTCACAGCCTCCATGGCAGCCCATCTGGACAACTCCCAGGCTTCAGAAAC CAAACTGGACATGATAAAGCCTGCATTGACTTCTGCAGTGGTGCCCAAGTCCATACTGGCTAAGCCATCC TCCTCTCCTGACCCGAGATACCTGTCAGTACCACCATCACCAAGCATTTCAGAATCACGATCCCCACCAG AAGGAGATACAACCCTCTTTTTGTCTCGACTCAACACGATTTGGAAAGGATTTATTAATATGCAGAGTGT AGCAAAATTTGTCACTAAGGCATATCCTGTCTCTGGGTGTTTGGATTATCTCAGTGAGGATTTGCCAGAC ACCATCCACATCGGTGGAAGGATTGCTCCAAAGACCGTGTGGGATTACGTTGGCAAACTCAAGTCATCGG TGTCCAAGGAGCTCTGTCTGATCCGCTTCCACCCTGCCACTGAGGAGGAGGAGGTTGCATACATTTCTCT CTACTCCTATTTTAGCAGCCGTGGTCGCTTTGGGGTTGTAGCTAATAACAACAGGCATGTCAAGGACCTG TACCTGATTCCACTGAGTGCTAAGGACCCTGTGCCATCCAAACTCTTGCCCTTTGAGGGCCCAGGTAAGC ACCCAGTTTCTGGGAGGTGATTAGGTCCACCCAGTCCCCACGCAGCCCTCTTCACTGACATAGTCCTCCC AGGTTGTCACAGGAACCTTGGCCTCCTGACCCAAAATCCCAGGAACTAAATTAGGATGACCAGTGAGTGG AATCATGTATGTATGGAGAATCCATTCTCTGAAATTTTAGACACATAGCTCTAAAAGTATCTCAGTTCGT ATGAAAATTGGCTCCCTGCTTATAATCAAATTTTTTGCTGCTTCAAACCAGAGAGCTGTTCAAATGTCAG GACTAAGAACCAAACTTAGAGAGGAGAGACAGGGCCTACCTGGATCCAGGGGTGCCTTCTACTCCAGAAA GTAGGTTGCTATTATTGTTGCCACTCTACTCAAAGTGAGTTGGCCTCTTTCATTCCAAGGGACTTCTCCA CAGGACTTGATTCCTAAGGAGTTCAGTAGCAGTGCCATAGAAAAGCATCCTAAAATGACTGCTTTTCAGT CACTGCACATATTGTAAGTTCTTTCATCTTTTGGATACTACTTAGATTTGCTCTTTCCTTGGAAGTATTG TTTAACCCATTACATACAAAGACACACAAGGTGGGGTCCAGATGACACAGAGGGCTTGGTCTGAGGTGTG TGGTCCTGCTGAGCGCCATCGAGGTGTCCTGTGGCCTCACTTAGACCATTGGTACTAGGGGCCTGAAATG GTCACTTCTGTCATGTTCCCAGAGGATTAATCTTACTAGCCAATGGGTTTGACATGTGCTAAATATTCTT GAACATGCTGTCATAGAAGTAGCTCCTGAATACCCCAAGGCCTGTACTCCACACCACTTGGACTACATAC ACCTAAACTGGCTAAGATGCTGAAGCCCAAGCCCAGTGCCCCAATCACAGCCCTCTACCAACTACAGACT GCTCCTAGTTCATGGCATGGCTTTACCTTTGCCTGCAGCTACACAGTATGTGTACATTAAGTGTTGGTTT GTGTCATAATGCTGGGTTAAATGTTAGTGGTATGATGCACTAGATGATTTTAGTGCCCAGCACATTTCAA AAGTAAAGTCTGTTAGTATGTTCTGTGAAGCCATAATCGGGATATAGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3979723 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3979723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19535 same embryo
	EMAGE:19537 same embryo
	EMAGE:19536 same embryo
	EMAGE:19534 same embryo
	EMAGE:19533 same embryo
	EurExpress:euxassay_004955 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source