EMAGE mouse gene expression spatial database

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EMAGE:19537

Mettl14 methyltransferase like 14 ( MGI:2442926)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004958. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004958_01	euxassay_004958_02	euxassay_004958_03	euxassay_004958_04

euxassay_004958_05	euxassay_004958_06	euxassay_004958_07	euxassay_004958_08	euxassay_004958_09

euxassay_004958_10	euxassay_004958_11	euxassay_004958_12	euxassay_004958_13	euxassay_004958_14

euxassay_004958_15	euxassay_004958_16	euxassay_004958_17	euxassay_004958_18	euxassay_004958_19

euxassay_004958_20	euxassay_004958_21	euxassay_004958_22	euxassay_004958_23	euxassay_004958_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19537_wholemount_strong.wlz

19537_wholemount_moderate.wlz

19537_wholemount_weak.wlz

19537_wholemount_possible.wlz

19537_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19537_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7998
Entity Detected:	Mettl14, methyltransferase like 14 ( MGI:2442926)
Sequence:	sense strand is shown >T7998 GTGAAAGTCTCCGGGTCAGAATCTCTGCGGGGCGGGACCCGGTGTTTCCTGGTTTGGCAGGTGGATTTGC ATTTTGGCGGGCGCGGAGTCCGTCGGGGTGGTGAGCAGCCGAAGCTGGGGCATGGATAGCCGCCTGCAGG AGATCCGGGAGCGGCAGAAGTTACGGCGGCAGCTCCTAGCTCAGCAGTTGGGAGCTGAGAGTGCGGATAG CATTGGTGCTGTGTTAAATAGCAAAGATGAACAGAGGGAGATTGCTGAAACCAGAGAAACCTGCAGGGCT TCCTATGATACATCTGCTCCAAACTCAAAACGGAAGTGTCTGGATGAAGGAGAGACTGATGAAGACAAAG TAGAAGAATATAAGGATGAACTGGAAATGCAGCAGGAGGAAGAGAATTTGCCATATGAAGAAGAGATTTA CAAAGATTCCAGTACCTTTCTTAAGGGAACGCAGAGCTTAAATCCCCATAATGATTACTGCCAACATTTT GTAGACACTGGACACAGACCTCAGAATTTCATCAGGGATGTAGGTTTAGCTGACAGATTTGAAGAATACC CTAAACTTAGGGAACTCATCAGACTAAAGGATGAGCTAATAGCTAAGTCAAACACTCCTCCCATGTACTT ACAAGCAGACATAGAAGCCTTTGACATCAGAGAATTGACACCCAAATTTGATGTGATTCTCCTGGAGCCC CCTCTGGAAGAATACTATAGAGAGACTGGCATCACTGCGAATGAGAAATGCTGGACCTGGGATGATATTA TGAAGTTAGAAATCGATGAGATTGCAGCACCTCGGTCATTTATATTTCTCTGGTGCGGTTCTGGGGAAGG ATTGGACCTTGGGAGAGTATGCTTGCGAAAGTGGGGTTACAGAAGATGTGAAGATATTTGTTGGATTAAA ACCAATAAAAACAATCCTGGAAAGACAAAGACTCTAGATCCAAAGGCAGTTTTCCAGAGAACAAAGGAGC ATTGCCTGATGGGGATCAAAGGAACCGTGAAGCGAAGCACAGACGGGGACTTCATTCATGCTAATGTTGA CATTGACTTAATTATCACAGAAGAACCTGAGATTGGCAATATAGAAAAACCAGTAGAAATTTTTCATATA ATAGAACATTTTTGTCTTGGTAGAAGACGCCTTCATCTCTTTGAGAGAGATAGCACTATCAGGCCAGGCT GGCTCACAGTTGGACCAACGCTTACAAACAGTAACTACAATGCAGAAACATATGCATCGTATTTCAGTGC CCCCAACTCATACTTGACCGGATGTACAGAGGAAATCGAGAGGCTTCGACCGAAGTCACCTCCTCCCAAG TCCAAGTCTGACCGTGGGGGTGGAGCTCCCAGAGGTGGAGGAAGGGGGGGAACATCTGCTGGCCGTGGTC GGGAAAGAAACCGATCCAATTTCCGAGGAGAGAGAGGTGGCTTTAGGGGGGGCCGTGGAGGCACGCACAG AGGCGGCTTTACTCCTCGGTAGCTCTTACACTTGCATCTTTATCACAGTGGAGACTTGCTTCAGAGCTCA CTCCCAGCTTGTACTTTGCTTTAGTTTCTCATGCTGCCAGAAAGATCTTAGCCACCCCACCCCACCCCAG GGACTGGCAGTTGTCACAGACTGAGCTTTTGCTCAGGGTGGGTGAGTCACGTGTGCGCAACACCCCGACC TGACTTGACTGATTCCAGTCAAGGCTTTGGCTTCCTGGAAATGGCTGCGCAGAGGCTTCGGCATCTCAGA GGAGTGGCAGGAGTGGGTGCTGTGTTCCCTGTCATGGCTGCACGGCAGCAGGAGGCTGGGAACCCGGGTA GAACAGAATTAGAGAGGTGGTCACTGTGTTGGGCCTTTAATGTTTTCTTGTTGAAACACTAAAAAGAGAA GAGTTTTTCCCCTTACTTTTAAATTCTAAAAAATAACATGAAGTATCATTTGAGTTCCCAGAACTCGGGA GGGGAAATGAAATCTCGTTCTTCCAGATCAGAATCTGAGGTGGTTGCAGTCGTCAGGGTAGGTAACGGGA GAGGCAGGCAGAGCATGGGATATTTCAAACAAAACCAAGCTTATCATAATGTAAATTAGCGGCTACAGAT GGCTCCTGACCATGTGTCTTCAGAAGCAAGACAAGGCATGTATGTCTCCAGGTCGGAGTGTGAACCTGAT CTTGGGCTAACCTAAGATAATTCAGATTTCTTAGCAAAGGCAAAAACAGATTTGGGGAGGGGCCGGTTTA AACGGTTGAGAAAATAAAATCTTGATGATGAGGAAATGACAAACATCTTTAAAGTTGTTACTAAAGAAAA TTTAGGTAAAAGCAATTCCAGAAAACCCTTACGAATACAGCGTGGAAGAGGACCTGCAGTGTCCTTTGAT TAGAACTATGTGGTGCAGAAGTCGCCGCAGGGCTGATGGGCAGATCTCTTGGCTGTGGGAAGCTTCGTGC TGTTGCCTTTCTACACCTTAAGTCCTATTTGATGGATTTGGATATGCCAGATGAGATAATGCCAATATTG TTCTGGGTAACTCCTTTTTTATTTTGCTATAACTTTTCTAATAAAAATTTAAACCTTTGAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:30007932 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30007932 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19535 same embryo
	EMAGE:19532 same embryo
	EMAGE:19536 same embryo
	EMAGE:19534 same embryo
	EMAGE:19533 same embryo
	EurExpress:euxassay_004958 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source