EMAGE mouse gene expression spatial database

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EMAGE:19613

Ttll12 tubulin tyrosine ligase-like family, member 12 ( MGI:3039573)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004979. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004979_01	euxassay_004979_02	euxassay_004979_03	euxassay_004979_04

euxassay_004979_05	euxassay_004979_06	euxassay_004979_07	euxassay_004979_08	euxassay_004979_09

euxassay_004979_10	euxassay_004979_11	euxassay_004979_12	euxassay_004979_13	euxassay_004979_14

euxassay_004979_15	euxassay_004979_16	euxassay_004979_17	euxassay_004979_18	euxassay_004979_19

euxassay_004979_20	euxassay_004979_21	euxassay_004979_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19613_wholemount_strong.wlz

19613_wholemount_moderate.wlz

19613_wholemount_weak.wlz

19613_wholemount_possible.wlz

19613_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19613_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 07 08 09 10

submandibular gland primordium

strong

regional

strong expression: see section 04 05 13 14 15

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 13 14

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

metanephros

strong

regional

strong expression: see section 03 04 05 06 07 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8051
Entity Detected:	Ttll12, tubulin tyrosine ligase-like family, member 12 ( MGI:3039573)
Sequence:	sense strand is shown >T8051 AAGAGGTAATCCATGGAGATCCAGTCCGGGCCGCAGCCTGGCAGTCCCGGCCGCGCCGAGAGGCTGAACG CGCGGCTGCTGGACGAGTTCGTGTCGCTGCACGGCCCTACGCTGCGCGCCTCGGGAGTCCCAGAGCGACT ATGGGGCCGCCTTCTGCACAAGCTGGAGCACGAGGTTTTCGATGCCGGGGAGATGTTTGGGATAAAGCAG GTGGAGGAGGTGGAGGAGGCTGAGGACGAGGCCGCCAGGGAGGCTCAGAGGAAGCAGCCCAACCCTGGGG GCGAGCTGTGCTACAAGGTCATTGTGACCAGTGAGAGCGGCGTCCGGGCTGACGACCCCAACAGCATCTT CCTCATCGACCATGCCTGGACGTGCCGTGTGGAGCATGCACGCAAACAGCTGCAGCAGGTACCTGGACTC CTGCACCGGATGGCCAACCTCATGGGCATCGAGTTCCATGGGGAAGTGCCCAGCCCAGAGGTTGTGGCCC TAGTGCTGGAGGAGATGTGGAAATTCAACCAGACCTATCAGCTGGCCCATGGGACAGCCGAAGAGAAGGT GCCAGTGTGGTACATCATGGACGAGTTTGGCTCGAGGATCCAGCACTCAGACATGCCCAGCTTCGCCACT GCTCCCTTCTTCTACATGCCCCAGCAGGTGGCATACACGCTGCTGTGGCCCCTAAGGGACCTAGACACAG GCGAGGAGGTGACCCGGGACTTTGCCTACGGAGAGGCAGACCCTCTGATCCGGAAATGCATGCTGCTGCC CTGGGCCCCCGCCGACATGCTGGACCTCAGCTTCTCTACGCCTGAGCCTCCTGCCAAGTACTACCAGGCC ATTTTGGAGGAGAACAAGGAGAAACTGCCGCTCGCCATTAGCCCAGTGGCACGTCCCCAGGGCCACGTCT TCAGAGTCCACTGCGACGTGCAGCAGGTGCTCGGCCACCTTACCCACCCTCGCTTCACCTTCACTGATAG CGAGGCTGATGCCGACATCTTCTTCCACTTCTCACACTTCAAGGACTACATGAAACTCAGCCAAGAGAGC CCACAGGTGCTACTCAACCAGTTCCCCTGTGAGAATCTGCTGACTGTTAAGGACTGTCTGGCCTCCATCG CTCGCCGGGCTGGGGGTCCTGAGGGTCCCCCCTGGCTACCCCGAACCTTCAACCTGCGCACTGAGCTGCC CCAGTTTGTCAGCTATTTCCAGCACCGGGAGAGGCGGGGTGAAGACAACCACTGGATCTGCAAGCCCTGG AACCTGGCCCGCAGCCTGGACACTCACGTCACCAACAACCTCCACAGTATCATTCGGCACCGAGAGAGCA CCCCCAAGGTTGTGTCCAAGTACATCGAAAGTCCCGTCTTGTTCCTCCGAGAAGATGTGGGGAACGTCAA GTTTGACATCCGCTACATTGTGCTGCTGCGCTCAGTGAGGCCGCTGAGGTTGTTTGCTTACGATGTGTTT TGGCTACGCTTCTCCAATCGGCCCTTCGCCCTCGATGACCTAGACGACTATGAGAAGCATTTCACCGTCA TGAACTATGACCCGGATGTGGTGCTGAAGCAGGTGCACTATAATGAGTTCATCCCTCAGTTTGAGAAGCA GTACCCAGAGTTTCCCTGGAGTGATGTTCAGGCTGAGATCTTCAAGGCCTTCACGGAGCTGTTCCAAGTG GCATGTGCCAAGCCACCGCCCATGGGCCTCTGTGACTACCCCTCATCCCGGGCCATGTATGCCATTGATC TCATGCTCAACTGGGACAACCACCCAGATGGAAAGCGGGTGATGCAGCCCCAAATCCTGGAGGTGAACTT CAACCCCGACTGCGAGCGAGCCTGCAGATACCACCCCTCATTCTTCAATGATGTCTTCAGCACTTTGTTT CTGGACGAGACTGACAACTGCCATGTCACCCGCATCATCTAGGTGCCCAGAGTTCTGCCTGTTCTCATAG CCAGTTCCAGCCGGTTCCAGCCGGTCCAGCCTCTGCTCACAGAACTGCCTTTGCAGTCCCAGGCTTCTCC CTCCCTCCCTCTCTCCCCTCCTTCCTTCCTCAGCCAGAGCCTTGGCCTAAGTTATACTCAACCCTCCTGA GCCGTGTTCCTGCTCCATATGTGGGATATCAGGTCCATGTGTGGCAGAGCTGGACAGTGAGTGGTGTCCC AAGGACTGGGCTCCGACCAGCTGTACCTGGTCTCTGTACCTCTTAACGCCCTCTCTTCTCTGAAGAGTCT GCAGCTCAGCTAAGGGCTTTTCTCCTGGCATCCAGAAAGTTCGCAGACCAAGAAATGTTCTGCAGAAACA GGGTTCGACGAGGGAAGTGTTTGGGAACCCGGTATCCACAAGGGCCAGGGAGTACTCTCAGAATCTCTGC CCTCCTGTCCCATGGCTGCTGCAAGCAGGGTACGGGAACCCTGTGTTGCACCCACAGGCCCCAGAACAGC CAAGGGGAAACATTTGCCAACCCGGCCTTATGCCTCGGCATCCTCCGCCAGAGCCTTCACCAGCAGACTT CTCAGCCATGCCTACCCTGGGTGTTTCCCTTCTGGGAAGCTGGGCTATGCAGAGTCCCATGGTCTCTGCA TGTTGGTACCTCACCAAGTCCCACTGTGCTCTTGTGGTAGACAGGGTGCTAGGTTGCTCTAGGGCTGTGA CATTTCATGGCAGAATCCAAGCAGGAGTGGCTGTGGAAATGCCAGAGCGGCTAAGATGGCTCTGGAGAGA ACTTCATGTGGGAATCAGGCTGCCTCTTGCCAAAGTTCAGAGTCCTAGTGTTAAGGTTTTGGGTTTGGTT TTCCTTCAACCTGCAGGAAAGACAGTGTTACGTGGAGTTCCTAATTTTGTTGCTGGAATTTGAAGTCAGC CACCATTGCTTCTGTAACTGCTGGGCGCTGGGATCCTGCCCCCAGGAAGTTAAGAGTGGGCAGGAGCTCC TTCCAGATGTCCTACTGTTCGGGTGGGACACTGTGGTGGCTGTTGTACTTGTGAAGGTAAAAGTGACCTT CTCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30055801 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30055801 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19609 same embryo
	EMAGE:19611 same embryo
	EMAGE:19608 same embryo
	EMAGE:19610 same embryo
	EMAGE:19612 same embryo
	EurExpress:euxassay_004979 same experiment
	MGI:4828998 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source