EMAGE mouse gene expression spatial database

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EMAGE:19657

Mmachc methylmalonic aciduria cblC type, with homocystinuria ( MGI:1914346)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005175. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005175_01	euxassay_005175_02	euxassay_005175_03	euxassay_005175_04

euxassay_005175_05	euxassay_005175_06	euxassay_005175_07	euxassay_005175_08	euxassay_005175_09

euxassay_005175_10	euxassay_005175_11	euxassay_005175_12	euxassay_005175_13	euxassay_005175_14

euxassay_005175_15	euxassay_005175_16	euxassay_005175_17	euxassay_005175_18	euxassay_005175_19

euxassay_005175_20	euxassay_005175_21	euxassay_005175_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19657_wholemount_strong.wlz

19657_wholemount_moderate.wlz

19657_wholemount_weak.wlz

19657_wholemount_possible.wlz

19657_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19657_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8842
Entity Detected:	Mmachc, methylmalonic aciduria cblC type, with homocystinuria ( MGI:1914346)
Sequence:	sense strand is shown >T8842 TGCGTTATGGAGCCGCGAGTCGCAGAGCTGAAGCAGAAGATTGAGGACACCTTGTGTCCTTTTGGCTTCG AAGTTTATCCCTTCCAGGTGGCGTGGTACAATGAACTCCTGCCTCCAGCCTTCCACTTGCCCTTCCCAGG ACCTACCCTGGCCTTCCTGGTACTCAGCACACCTGCTATGTTTGACAGAGCCCTCAAACCCTTCTTAAAG AGCTGCCACTTCCAAACACTGAGAGACCCGGTGGATCAATGTGTGTCCTACCACCTGAGGAGTGTTACAG AGAAGTTTCCAGAAGTGCATATGGAAGTCATTGCTGACTATGAGGTACACCCCAATCGGCGACCTAAGAT TCTCGCCCAGACAGCAGCCCATGTGGCAGGTGCTGCTTATTACTACCAACGACAAGATGTGGATGCAGAC CCATGGGGGACCCAGCACATAGCAGGTGTGTGCATACACCCCCGATTTGGGGGCTGGTTTGCCATCCGAG GGGTTATGTTGCTGCCAGGGATTGAAGTGCCAAATTTGCCACCCAGAAAGCCCCCTGACTGTGTGCCTAC AAGAGCTGGCCGCATCACTCTGCTTGAAGGTTTCAATTTCCATTGGCGGGACTGGACTTACCGTGATGCT GTGACTCCTGAAGAACGGTACTCCGAAGAACAGAAGATCTACTTTTCCACCCCACCTGCCCAACGCTTGG CCCTATTAGGCTTAGCCCAACCCTCAGAACACCCTAGCACTACATCTGAGCTTCCTCTTTCCTTGCTTAC TAAACCTCAGAATTCCAGGAGAGCTCGAAGCTGGCTGAGTCCAAGTGTCTCACCACCTGTATCCCCAGGC CCTTGATAACTTTTCCCCTTCAGGATCCCACTCATAGTAGATTTATTTTAAGTAGGATTTGGGTTTTGAA AAAAACACTTTCTCCTTTTTTAGTTTTTTTGAGACAAGGTTCCTCTATGTAGCTCAGACCAGGTTGGCCT CAAGCTAAGAGAGATCCATCTGCCTCTGTCTTCTCCAGAGTGCTGATTTTTTGCACTGTGGAAAAACCAT TTTATTTTAGGTAGGATTTCCTGATAATAGTGCTTCAGACAGTCAAGACTCAATTTACATTGGTCAAGAT AAATCATATGCCAAGGCAGACTCAAAAATCAGCAGTTGACTTCAAATATTAGACTCATGCCCATTACTAT TTTTTTGTTTTTGTTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCA CTCTGTTGACCAGGCTGGCCTCAAACTCAGAAATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAG GCGTGCACTACCAAGCCCGGCTTTGTGTAGTACTCTTAAGATACTATTTAAGAACCATGGTTCTCACTGT TCGTTCCTAAAGCTGCTATCATTTAATGCAGTTCCTCATGTTGTAGTGACCGCAACCATAAAATTATTTT TGTTGCTACCTAATAATTTTGCTAGTTATGAATCAATTGTCTTAGGGAATCCAGTGAAAGGGTTGTTCAA CAACCCCTGGGGGCTGAGAATCACAGATTTAGCAGCTCCTAGGGGTACCCACCACATTGAGCTATGCTGA ATTAATATTTAAGTAAACTACATTGAAACTGCCCCGACCTACAGTGTGCTATTACTAAAGGCTCAGGCAA TCTTATACTTATGCTCAAATAGATGTACTTTGAATTCTGGAATGTTGGAAGGACATGATGTCATAGGATT ATTTTGTCAACCCTAACCAAGAATCCAGAAGAAACTTCCTGACTTTACAGGATCAGAAAGGAATATTCTC TCATCACAGCTAAAATACAAGTAGTTGAATACAATCATATCCAAAGCCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6411797 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6411797 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19656 same embryo
	EMAGE:19658 same embryo
	EMAGE:19655 same embryo
	EMAGE:19654 same embryo
	EurExpress:euxassay_005175 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source