EMAGE mouse gene expression spatial database

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EMAGE:19684

Tceal6 transcription elongation factor A (SII)-like 6 ( MGI:1923939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005197. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005197_01	euxassay_005197_02	euxassay_005197_03	euxassay_005197_04

euxassay_005197_05	euxassay_005197_06	euxassay_005197_07	euxassay_005197_08	euxassay_005197_09

euxassay_005197_10	euxassay_005197_11	euxassay_005197_12	euxassay_005197_13	euxassay_005197_14

euxassay_005197_15	euxassay_005197_16	euxassay_005197_17	euxassay_005197_18	euxassay_005197_19

euxassay_005197_20	euxassay_005197_21	euxassay_005197_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19684_wholemount_strong.wlz

19684_wholemount_moderate.wlz

19684_wholemount_weak.wlz

19684_wholemount_possible.wlz

19684_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19684_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 03 04 05 06 22

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 22

vertebral axis musculature

strong

regional

strong expression: see section 05 06 07 21 22 moderate expression: see section 01 02 03 04 08 09 10 11 12 13 14 15 16 17 18 19 20

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

tail mesenchyme

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9630
Entity Detected:	Tceal6, transcription elongation factor A (SII)-like 6 ( MGI:1923939)
Sequence:	sense strand is shown >T9630 GGTGCCCACGTGGGCACCAGCCCCCGCGCCAGCTAGTCCTGCTGCTGCTGCTGCTGCGGATACCTGTGCC CAACACCCGCATTTGCGCAGCACCGTTTCCTTGAGCTGTCCTGGTTACTGTGAGCCCAGAGAAGGTGCAC AGGCCTGTCCTGGGTCTCCTCAGGTCTCTGCTGTTCCCAGTGCATTGTGACATCTGCAAAAGAGGGTCGA CCTGCCCAGGATCCCTGCAGGACAGGAAAAGGAGGGAAATCTTGACATGGAAGAAGTCCGCGGTGAAAAC GAAGGCAAGCTGGAAAAGGAGGGAAAGCCAGAAGATGAAGTAGAGCCTGAAGATGAAGAAAAGTCAGATG AGGAAGAGAAGCCAGACAAGAAAGCAAAGCCAGCACCACGCCAGGGCAAGCCAGAGGAGGAGGCAAAGCC AGATGAGCAGGGGCAAGATGAGGGGAAGCCCGAGAAGCAGGGAAAGTCTGACGGGGAGGGCAAGCGCCAA GGGGAGAGCAAGCCCGATTCCCAGGCAAAGTCAGCCAGCGAGGCGCGGGCTGCAGAAAAGCGCCCTGCTG AAGATTATGTGCCCCGGAAAGCAAAACGAAAAACAGATAGGGGGACAGATGATTCTCCCAAGAACTCCCA GGAGGACTTACAGGACAGGCATGTAAGCAGCGAGGAGATGATGAGAGAGTGCACAGATATGACTAGGGCT CGGGAAGAGTTAAGGAAGAGGCAGAAAATGGGTGGTTTTCACTGGGTGCCAAGAGATGCACAGGATGCTT TAGTCCCCAGGGGCCAGCGGGGAGTCAGGGGGGTGAGGGGCGGCGGAGGCAGGAGCCAGAGGGGCCTACA TGACATCCCATACCTTTAATGCCTTTGGCCATCAATTCTGACCTCTCTGAGGAGAAAGAACACTGCTGGC CCTGCTTTTCCTGGTAGATACTTGCCAGGCCTGTGCTTTAACCTTCAGCCAAGACTCTGCTTCAGGTGTC ACTGTCCTTACCAGCAGCCTTCTGACCCAACTCCAGTGCTCTCTGTCTTTTAGTACAGGATTTTCACCCA TGTGCATGAAAAATATGTTCATGGTACATTGTAAAAATGAATAAACAAAAATTTGCAGAACCTCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6533845 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6533845 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19682 same embryo
	EMAGE:19686 same embryo
	EMAGE:19683 same embryo
	EMAGE:19685 same embryo
	EurExpress:euxassay_005197 same experiment
	MGI:4828631 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source