EMAGE mouse gene expression spatial database

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EMAGE:19700

Zfp647 zinc finger protein 647 ( MGI:3052806)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006176. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006176_01	euxassay_006176_02	euxassay_006176_03	euxassay_006176_04

euxassay_006176_05	euxassay_006176_06	euxassay_006176_07	euxassay_006176_08	euxassay_006176_09

euxassay_006176_10	euxassay_006176_11	euxassay_006176_12	euxassay_006176_13	euxassay_006176_14

euxassay_006176_15	euxassay_006176_16	euxassay_006176_17	euxassay_006176_18	euxassay_006176_19

euxassay_006176_20	euxassay_006176_21	euxassay_006176_22	euxassay_006176_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19700_wholemount_strong.wlz

19700_wholemount_moderate.wlz

19700_wholemount_weak.wlz

19700_wholemount_possible.wlz

19700_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19700_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

diencephalon

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

hypothalamus

strong

regional

strong expression: see section 11 12 14

telencephalon

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata lateral wall

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 14 15

metencephalon lateral wall

moderate

regional

moderate expression: see section 06 10 11 13 14 15 16 17 18 19 20 21

pons mantle layer

strong

regional

strong expression: see section 08 17

midbrain

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain lateral wall

moderate

regional

moderate expression: see section 07 08 09 12

tegmentum

strong

regional

strong expression: see section 11 12 14

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 15 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 17 weak expression: see section 08 16

trigeminal v nerve

strong

regional

strong expression: see section 09 10 16

spinal cord lateral wall

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 13 14 15

not examined

regional

not examined expression: see section 01 02 03 04 22 23

retina

moderate

regional

moderate expression: see section 01 02 03 04 22 23

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8863
Entity Detected:	Zfp647, zinc finger protein 647 ( MGI:3052806)
Sequence:	sense strand is shown >T8863 GAAGCTGCCGCCACAGGGCGAGAGGTTGCGGCAGATGCCCCACAGGATCGCCTGATTTCTGTTGTCCTCG CAGTCTTGAGATACCAAGATGGCAGCAGCCGGGCTTCTACCGCTGCCAGCAGCACCACAGGCCAAAGTGA CCTTTGAGGATGTGGCTGTTCTGCTGTCTCAGGAGGAGTGGGCCCGCCTGGGCCCTGCTCAGCGGGGCCT CTACCGACACGTCATGATGGAGACCTACGGGAATGTAGTCTCACTGGGACTTCCAGGATCTAAGCCTGTT GTGATCTCCCAGCTGGAGCGAGGAGAAGACCCGTGGGTCCTGGATGGACAGGGAACCGAGCTGAGCCAGA GCCTGGGAAGTGACCACTCAGAATGCAAAGCCAAGGAAGAGAACCAAAACACAGACTTGAATGTGCCACC ATTAATCTCAGACGAAGCATCAGCGACGCTGACGGAAACACCGCTGAGGAAGGTTGCTGAAGAACGTTAC AAAACAGAACCGAAGGTCTGCCCCAGTCCAAAGCCCATTGGCCCCCAGAATGCCCACGGGTTGAACCCTA GTGTACCCGTTGCCCGACCCCAGACGGCTCCCAGTGTTGAGAGACCCTATATCTGCATTGAGTGTGGAAA GTGCTTTGGTCGGAGCTCCCATCTCCTTCAGCATCAGCGAATACACACTGGCGAGAAGCCCTACGTATGC CATGTATGCGGAAAGGCTTTCAGCCAGAGTTCCGTCCTGAGCAAGCACAGGCGGATCCACACGGGCGAGA AGCCCTACGAGTGTAACGAATGTGGGAAAGCCTTTCGCGTGAGTTCCGACCTTGCCCAGCACCACAAGAT CCACACCGGAGAGAAGCCTCACGAGTGTCTGGAATGTGGGAAGGCGTTCACCCAGCTCTCCCACCTCATC CAACATCAGCGGATCCACACGGGGGAGCGGCCCTACGTGTGTCCCTTGTGTGGGAAAGCCTTCAACCACA GCACCGTCCTGCGGAGCCACCAGAGGGTGCACACTGGGGAGAAGCCTCACGGGTGCAGCGAGTGTGGGAA GACCTTCAGCGTGAAGAGGACGCTGCTGCAGCACCAGCGGGTGCACACCGGGGAGAAGCCCTACACGTGC AGCGAGTGCGGGAAGGCCTTCAGCGACCGCTCCGTGCTCATCCAACATCACAACGTACACACTGGGGAGA AGCCGTACGAGTGCAGCGAGTGCGGCAAGACCTTTAGCCACCGCTCCACCCTGATGAATCACGAGAGGAT TCACACGCAGGAGAAGCCCTACGCGTGCTACGAGTGCGGGAAGGCCTTTGTCCAGCACTCGCATCTCATC CAGCATCAGAGAGTCCACACGGGAGAGAAACCCTACGTGTGTGGCGAGTGTGGCCATGCTTTCAGCGCAC GCCGGTCCCTGATCCAGCATGAGCGAATCCACACAGGCGAGAAACCCTTTCAGTGCACAGAGTGTGGCAA AGCCTTCAGCCTGAAAGCAACTCTGATCGTGCACCTGAGGACCCACACGGGCGAGAAACCCTATGAGTGC AATAGCTGCGGCAAGGCGTTCAGCCAGTACTCGGTGCTCATCCAACACCAGAGGATCCACACCGGAGAGA AACCCTATGAGTGTGGCGAGTGCGGACGGGCCTTCAACCAGCACGGCCACCTGATCCAACATCAGAAAGT ACACAAGAAGCTGTGACCCATGGCTGTCAGAAGCCCGCACTTAGAACATGCACAAGACCTAAACAGCCTT CCTCCAGGAGGGAGTTCTGTTCCATGTGCAGAATCGTCGTTCCCTTGGGGACTCTGTTACAGTCCCATGT CCCTCCTGGGGAGAAACATTCAGCATGTGTTAAATTTATTTTCCTCAACATCTTGGAAGTTAAACTGGAG AGTAATCCTACAACTGTAGATACTGGTAAAGACAGCCATAATTCTACCTTTGTCTATTTGACATGAATTA GTTTATTGAAGCAACTGAAAAGCTATAAAGCTTAGGGACTATTCACATTTCAAATGAAGTTCCTTTTACA CAGCTTCATGACGTTCCTAAATGTATCTGATCATTCTAATATTGTGCTTAAATTTAGAATGTTGGACCAT TTTCGGTATCCTTTTAAAACACTTAAGTGCTATGTTGATAAAGTAATATTAAAAAAAAAAAAAGATCTTG GGCCAGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414212 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414212 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19697 same embryo
	EMAGE:19699 same embryo
	EMAGE:19698 same embryo
	EurExpress:euxassay_006176 same experiment
	MGI:4829328 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source