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EMAGE:20262

Scnm1 sodium channel modifier 1 ( MGI:1341284)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20262 EMAGE:20262 EMAGE:20262 EMAGE:20262 EMAGE:20262
"Pseudo-wholemount" of euxassay_015889. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015889_01 euxassay_015889_02 euxassay_015889_03 euxassay_015889_04
EMAGE:20262 EMAGE:20262 EMAGE:20262 EMAGE:20262 EMAGE:20262
euxassay_015889_05 euxassay_015889_06 euxassay_015889_07 euxassay_015889_08 euxassay_015889_09
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euxassay_015889_10 euxassay_015889_11 euxassay_015889_12 euxassay_015889_13 euxassay_015889_14
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euxassay_015889_15 euxassay_015889_16 euxassay_015889_17 euxassay_015889_18 euxassay_015889_19
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euxassay_015889_20 euxassay_015889_21 euxassay_015889_22 euxassay_015889_23 euxassay_015889_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20262Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20262_wholemount_strong.wlz
20262_wholemount_moderate.wlz
20262_wholemount_weak.wlz
20262_wholemount_possible.wlz
20262_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20262_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31258
Entity Detected:Scnm1, sodium channel modifier 1 ( MGI:1341284)
Sequence:sense strand is shown

>T31258
ACGGTGGCCTAAAGGAGCAGCGAAGTAGGCGTAGGGGAATTTATCGTAGTCTCACAGTCGCTATGTCTTT
TAAGAGGGAAGGGGACGACTGGAGTCAACTCAATGTGCTCAAAAAACGGAGAGTTGGGGACCTGCTGGCT
AGTTACATCCCTGAGGACGAGGCACTGATGCTGCGGGATGGACGCTTTGCTTGTGCCATCTGCCCCCATC
GACCAGTACTAGACACGCTGGCCATGTTGACAGCCCACCGTGCAGGCAAGAAGCATTTGTCCAGTCTGAA
GCTTTTCTATGGCAAAAAGCAAACAGGCAAGGGAACAGAGCAAAATCCAAGACAGCAGAACGAATTGAAG
ACAGAAAGCAAAACTGAGGCTCCTTTGCTAACCCAGACTCGAATCATCACCCAGAATGCTCTACACAGAG
CTCCCCACTATAACAGTTGCTGCCGGAGGAAGCACAGACCAGAAGCCCCTGCTCCCTCTGTCTCCAGTCC
TCCTTTGCCAACTGCAGAGGTCCAACTCCAGAGTGCAGAGATCAGTAAGGAACCTGAGCCTAGGGAGAGA
TCAGATGCCAAAGAGTCAGCAGCTCTCTTGGCCTCTGCACCCATGAGCCCCACCAAACGACGAGTCCTGA
ACCATTACCTTACCCTCCGAAGCTCTGGATGGGTCCCAGATGGACGAGGTCGATGGATAAAGGATGAAAA
TGTTGAGTTTGACTCTGATGAGGAAGAGCCCCCCGATCTCCCCTTGGACTAACATTAATAACCCCTTTCC
CCATTTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4220000), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55720. Forward Primer - name:055720_F_IRAV49_e01_Scnm1, sequence:ACGGTGGCCTAAAGGAGC; Reverse Primer - name:055720_R_SP6_IRAV49_e01_Scnm1, sequence:GACAAATGGGGAAAGGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20261 same embryo
 EMAGE:20260 same embryo
 EMAGE:20264 same embryo
 EMAGE:20263 same embryo
 EMAGE:20259 same embryo
 EurExpress:euxassay_015889 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS