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EMAGE:20527

Chek2 CHK2 checkpoint homolog (S. pombe) ( MGI:1355321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527
"Pseudo-wholemount" of euxassay_012795. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012795_01 euxassay_012795_02 euxassay_012795_03 euxassay_012795_04
EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527
euxassay_012795_05 euxassay_012795_06 euxassay_012795_07 euxassay_012795_08 euxassay_012795_09
EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527
euxassay_012795_10 euxassay_012795_11 euxassay_012795_12 euxassay_012795_13 euxassay_012795_14
EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527 EMAGE:20527
euxassay_012795_15 euxassay_012795_16 euxassay_012795_17 euxassay_012795_18 euxassay_012795_19
EMAGE:20527
euxassay_012795_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20527Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20527_wholemount_strong.wlz
20527_wholemount_moderate.wlz
20527_wholemount_weak.wlz
20527_wholemount_possible.wlz
20527_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20527_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32158
Entity Detected:Chek2, CHK2 checkpoint homolog (S. pombe) ( MGI:1355321)
Sequence:sense strand is shown

>T32158
GCTCATCGAGAGAAGCCGACACAGCTCCCAGTGTGGAAACTGAAATAGAAATTTTGAAGAAACTAAATCA
TCCATGCATCATCAAGATTAAAGATGTTTTTGATGCGGAAGATTATTACATTGTTCTGGAACTGATGGAA
GGAGGAGAACTATTTGACCGGGTGGTGGGGAACAAGCGCCTGAAAGAAGCCACCTGTAAGCTCTACTTCT
ACCAGATGCTTGTAGCTGTACAGTACCTTCACGAAAATGGGATCATACATCGGGACTTAAAGCCGGAGAA
TGTTCTTTTATCATCTCAGGAAGAGGATTGTCTAATCAAGATCACTGACTTTGGGCAGTCCAAGATCTTG
GGGGAGACCTCCTTGATGAGAACCTTATGTGGTACGCCCACTTATCTGGCTCCTGAGGTTCTTGTCTCCA
ACGGGACTGCTGGGTACAGCCGCGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTCATCTGCCTAAG
TGGGTATCCACCTTTCTCTGAGCATAAGACCCAAGTGTCCCTGAAGGATCAGATCACCAGTGGAAAGTAC
AACTTTATTCCTGAAGTCTGGACAGATGTCTCAGAGGAGGCTCTGGACCTTGTCAAGAAACTGTTAGTTG
TAGACCCAAAGGCTCGGCTTACCACAGAGGAGGCCTTAAATCATCCGTGGCTTCAGGATGAGTACATGAA
GAAGAAATTTCAGGATCTCCTGGTGCAGGAAAAGAACTCGGTGACCCTCCCTGTGGCTCCCGCCCAGACT
TCCAGTCAAAAGCGGCCCCTGGAACTGGAGGTGGAGGGTATGCCGAGCACAAAACGCCTGTCTGTGTGTG
GGGCTGTGTTGTGAGCTTTGAATGTGAAAGAAATGTGCTTCCTTCCACGCTCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4977960), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60154. Forward Primer - name:060154_F_IRAV99_d10_Chek2, sequence:GCTCATCGAGAGAAGCCG; Reverse Primer - name:060154_R_SP6_IRAV99_d10_Chek2, sequence:TTGGAGCGTGGAAGGAAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20528 same embryo
 EMAGE:20526 same embryo
 EMAGE:20529 same embryo
 EMAGE:20530 same embryo
 EurExpress:euxassay_012795 same experiment
 MGI:4823842 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS