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EMAGE:20528

Cramp1l Crm, cramped-like (Drosophila) ( MGI:1930190)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528
"Pseudo-wholemount" of euxassay_012775. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012775_01 euxassay_012775_02 euxassay_012775_03 euxassay_012775_04
EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528
euxassay_012775_05 euxassay_012775_06 euxassay_012775_07 euxassay_012775_08 euxassay_012775_09
EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528
euxassay_012775_10 euxassay_012775_11 euxassay_012775_12 euxassay_012775_13 euxassay_012775_14
EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528
euxassay_012775_15 euxassay_012775_16 euxassay_012775_17 euxassay_012775_18 euxassay_012775_19
EMAGE:20528 EMAGE:20528 EMAGE:20528 EMAGE:20528
euxassay_012775_20 euxassay_012775_21 euxassay_012775_22 euxassay_012775_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20528Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20528_wholemount_strong.wlz
20528_wholemount_moderate.wlz
20528_wholemount_weak.wlz
20528_wholemount_possible.wlz
20528_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20528_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32155
Entity Detected:Cramp1l, Crm, cramped-like (Drosophila) ( MGI:1930190)
Sequence:sense strand is shown

>T32155
CTCCTCCTCCCGGAACATAGGATCTTCAGGGCCTGAGAAAGAAGAAGGAAAGAAGGTCCGAAGGCAGTGG
GAGTCATGGAGCACCGAGGACAAGAACACCTTCTTCGAGGGACTGTATGAGCATGGAAAAGACTTTGAAG
CCATTCAGAACAACATTGCTCTAAAGTACAAGAAGAAGGGCAAGCCTGCAAGCATGGTGAAGAACAAAGA
GCAGGTCCGGCATTTTTACTACCGCACCTGGCACAAGATTACCAAATACATTGACTTTGACAATGTGTTC
TCTCGAGGGCTGAAGAAGTCATCCCAGGAACTCTATGGTCTCATCTGCTATGGCGAGCTACGCAAGAAGA
TTGGGGGTTGTATGGATGACAAGAATGCAACAAAGTTGAATGAACTCATTCAGGTTGGAGCCACCACAGT
ACGTTATAAAGGGAGAAATCTCCGGATCAAGGCACCCATGTGCCGAGCCCTAAAGAAACTCTGTGATCCA
GATGGTCTAAGTGATGAAGAGGACCAGAAGCCAGTGCGCCTACCCCTGAAAGTCCCAGTAGAATTACAAC
CAAGGAACAACCACGCTTGGGCACGAGTACAGAGCCTGGCCCAGAACCCACGTCTCAGGATGATCGTGGA
GCTACATAGAAAAGTCTCTAGCCTCATTGAGTTCCTGAAGCAGAAGTGGGCACTCCATGAAGTACGAATT
CGGAAGACACTTGAGGAGAGGCAGCTGCAGGGTTCAAGCACAGTGCAGACCCAGGAGAAGGTGGCATTGC
ACCTGTTCCCAGGCGAGAACTGCACGCTGACACC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5340112), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60139. Forward Primer - name:060139_F_IRAV99_a05_Tce4, sequence:CTCCTCCTCCCGGAACAT; Reverse Primer - name:060139_R_SP6_IRAV99_a05_Tce4, sequence:TGGTGTCAGCGTGCAGTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20526 same embryo
 EMAGE:20527 same embryo
 EMAGE:20529 same embryo
 EMAGE:20530 same embryo
 EurExpress:euxassay_012775 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS