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EMAGE:20592

Gimap9 GTPase, IMAP family member 9 ( MGI:3511744)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592
"Pseudo-wholemount" of euxassay_009112. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009112_01 euxassay_009112_02 euxassay_009112_03 euxassay_009112_04
EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592
euxassay_009112_05 euxassay_009112_06 euxassay_009112_07 euxassay_009112_08 euxassay_009112_09
EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592
euxassay_009112_10 euxassay_009112_11 euxassay_009112_12 euxassay_009112_13 euxassay_009112_14
EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592 EMAGE:20592
euxassay_009112_15 euxassay_009112_16 euxassay_009112_17 euxassay_009112_18 euxassay_009112_19
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euxassay_009112_20 euxassay_009112_21 euxassay_009112_22 euxassay_009112_23 euxassay_009112_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20592Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20592_wholemount_strong.wlz
20592_wholemount_moderate.wlz
20592_wholemount_weak.wlz
20592_wholemount_possible.wlz
20592_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20592_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1197
Entity Detected:Gimap9, GTPase, IMAP family member 9 ( MGI:3511744)
Sequence:sense strand is shown

>T1197
CCTCGAGCCTGTTGGCCTACTGGACCCATAGAACTTCCTGTCAGGGAATGAGGGAAGTTTTACTTCATAG
TTCAGTAGAAAACTTGAACCAGGTCAAATTGGTTCCTTGGAGAGATCCTGAACTCTCATTAACTGTTGCA
GAAGAAGATCTGTGCATTCAGATCTTTAGAGTCAGCTTGCCTTGACATGGCTGGCCAGGGAGACACTGAA
GTGAGGATCATTCTGGTAGGGAAGACTGGAAATGGGAAGAGTGCTACAGCCAACACCATCCTCGGGAGAC
GTCAATTTGATTCTAAGATTTGTGCCAATGCTGTTACCAAGACCTGCCAGAGAGCATACAGGGAGTGGAA
GGAGAAAAACCTTGTTGTAGTCGACACTCCTGGGCTCTTTGACACCAAGGAGACCATGAAAACCACACGT
TTCGAGATCAGCCGCTGCGTTCTCTACTCCTGCCCTGGGCCTCACGCCATTATTCTGGTTTTGCGACTGG
ACCGTTACACTGAAGAAGAGCAGAAAACGGTCGCTCTGATCAAGGGTCTTTTTGGGGAGGCAGCCCTGAA
GTACAT
Notes:The probe template was PCR amplified from IMAGE:2182338 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182338 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20593 same embryo
 EurExpress:euxassay_009112 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS