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EMAGE:20708

Tiaf2 TGF-beta1-induced anti-apoptotic factor 2 ( MGI:2651383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20708 EMAGE:20708 EMAGE:20708 EMAGE:20708 EMAGE:20708
"Pseudo-wholemount" of euxassay_014012. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014012_01 euxassay_014012_02 euxassay_014012_03 euxassay_014012_04
EMAGE:20708 EMAGE:20708 EMAGE:20708 EMAGE:20708 EMAGE:20708
euxassay_014012_05 euxassay_014012_06 euxassay_014012_07 euxassay_014012_08 euxassay_014012_09
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euxassay_014012_10 euxassay_014012_11 euxassay_014012_12 euxassay_014012_13 euxassay_014012_14
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euxassay_014012_15 euxassay_014012_16 euxassay_014012_17 euxassay_014012_18 euxassay_014012_19
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euxassay_014012_20 euxassay_014012_21 euxassay_014012_22 euxassay_014012_23 euxassay_014012_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20708Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20708_wholemount_strong.wlz
20708_wholemount_moderate.wlz
20708_wholemount_weak.wlz
20708_wholemount_possible.wlz
20708_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20708_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39504
Entity Detected:Tiaf2, TGF-beta1-induced anti-apoptotic factor 2 ( MGI:2651383)
Sequence:sense strand is shown

>T39504
TCCCCCTTACTCTGATAGGTGACTCCATTTGGTTTAAATATTCATTGGATGTCTGAAGGTTGGTGTTTCC
AGCCATTGATCATGGGGACATGCTGTAATTTATTTAACCACTCCTCGGTGGCTAGGTTTCATCCCTGTAG
CTGGCAATGCTGTGAAAACTGTTTTGTGTGTATGTAGAAATCTTTGATTACATTGATATTTTCTTGGTCA
CATGTGTGTGTAATATACCTGTGCAAGGCCTCTGCAGTTGCCATACTTCTTGGGAACTGCCCTGTCCCAG
CATGGAGTGAGGGTTTCCTGCCTTGGAGCTAGTTGATGGCCCGTGACTTCAATGCTAAAACTGCATTTTA
GCAATCAAGGCACATGGTCTGTAGCTCCATGGTTTCTTTTATTGAAAGCACATTAAAATGCTGCTTGTAT
TACTTTAAAAACCAATTCTGGATTTTCTTCTTCTTTTCCTCCTCCTCCTCCTCTTCCTCTTCCTCCTCTT
CCTTCTTCTTCTTTTGTGGTTTTGCCCTGTAAAGGAACATGGCCTGTGGGGTCTCTGGGTCAGGTGCATT
TCCTTGGAAGAGTGATGTAGGTGATTCTGTAGAGAAATGCAACAGTGATGTTTAGGGGAACTGGAAGGGG
AGTGTGTCCTCCCCTCTACTGTGATGAGCACAGCATCTTCTATGTTTCCTGCAATTGCCTCTTATGTAGG
CTGGAAGAGTGGGGGAGAGAAGAGAGAGCGGGAGTGGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92819. Forward Primer - name:092819_F_cDNA_Tiaf2, sequence:TCCCCCTTACTCTGATAGGTGA; Reverse Primer - name:092819_N_SP6_cDNA_Tiaf2, sequence:CTCCACTCCCGCTCTCTCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20709 same embryo
 EMAGE:20710 same embryo
 EMAGE:20711 same embryo
 EMAGE:20707 same embryo
 EurExpress:euxassay_014012 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS