EMAGE mouse gene expression spatial database

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EMAGE:21076

Rgmb RGM domain family, member B ( MGI:1916049)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011645. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011645_01	euxassay_011645_02	euxassay_011645_03	euxassay_011645_04

euxassay_011645_05	euxassay_011645_06	euxassay_011645_07	euxassay_011645_08	euxassay_011645_09

euxassay_011645_10	euxassay_011645_11	euxassay_011645_12	euxassay_011645_13	euxassay_011645_14

euxassay_011645_15	euxassay_011645_16	euxassay_011645_17	euxassay_011645_18	euxassay_011645_19

euxassay_011645_20	euxassay_011645_21	euxassay_011645_22	euxassay_011645_23	euxassay_011645_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21076_wholemount_strong.wlz

21076_wholemount_moderate.wlz

21076_wholemount_weak.wlz

21076_wholemount_possible.wlz

21076_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21076_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

weak

regional

weak expression: see section 1 2 5 6 7

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 1 2 3 4 5 6 7

olfactory cortex mantle layer

weak

regional

weak expression: see section 0 1 2 5 6 7 8

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 9 0 1 9 0

pons mantle layer

weak

regional

weak expression: see section 7 8 9 0 1 8 9 0 1

midbrain mantle layer

weak

regional

weak expression: see section 0 1 2 9 0

dorsal root ganglion

weak

regional

weak expression: see section 7

esophagus

weak

regional

weak expression: see section 4 5

stomach

weak

regional

weak expression: see section 6 7 8 9 0 1 2

hindgut

weak

regional

weak expression: see section 6 7 8

midgut

weak

regional

weak expression: see section 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

axial skeleton tail region

weak

regional

weak expression: see section 9 0 1 2 3 4 5 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31705
Entity Detected:	Rgmb, RGM domain family, member B ( MGI:1916049)
Sequence:	sense strand is shown >T31705 GTGACGAACGTGCCTGTGGTCCCCGGGTCCAGTGCAACTGCTACAAACAAGGCGAGTATGCATCTTCCCC CGCCCCCCTTTCCATTCAAGTTGTTCGGTCCTTCAGACAACTTATAAAATGTGTTGTTATAAAGGCCTAG TTTAGAGGAACCATTTGTATATCTTCTTGGAAAGTTATTAACAGAGTTGGTAAAGGTTTGAAATTATTTA ATGAGAGATTTTCTCTGGTATGTAAGATGTGGGTCTAAACTTAACTAGACAATGATAGGAAGGATAAAAC GTAAACAGTGAAATCAAGTTCTCCAGGGAGAAAAGGCGGGCCTGTTTAATGAGAAAAACATGTGCCCTCC CCACTGAGGTCTTGTGAGAGCTCATGTGTGTGTGAGAGACTCTTCCCGAAGAAATCCACGTTCAAATTCA TCCGATGATGAAAGTGGGTGTTCTGAATAAAGAAAGCTGTGAAGCCTGGGGCAGTGGCCCATCAGGAAAC GTACTTTATTGGAGAGTTAGAAGCTATAGTAAATGTTATGGCCATAAGCCAGGCTTCCCAGTGGCAAACC CACAATGGTGCTTTAGTTACTAACCTCTTGGTAACCAAGGAAGTTAAGGATTGGCTGTTGCAGAACAGTG CTGTATAAGCCCTGTGTAATCTCACACCCTCTTTTAACACTGTAAGTCAGAAGCTAAGAAGAAAGTTGGC AAAATTACGTTAGGCTTACCCTGAACCAGTGTCTGTGCTCCTTGTTTGTTTATTCTGAAGAAATTGATCA TGTATAGGGAGAGACCTTTGATACCAGCTGCAAGGTACTGATATCTTCCTGCAATGCAAGGTGGAAGAAA GTATGGAGAGGGTCTCCAAGTACCAGTCAGGGCTAAGTAACTTACCATTCGTAAATAAAGTCAAAGGAGT TTCTCCCAGTGTCCCACTAAACTCCCTCCTTCCCTGTCTTCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5351870), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 32629. Forward Primer - name:032629_F_IRAV81-84_O23_Rgmb, sequence:GTGACGAACGTGCCTGTG; Reverse Primer - name:032629_R_SP6_IRAV81-84_O23_Rgmb, sequence:GGGGAAGACAGGGAAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011645 same experiment
	EMAGE:21072 same embryo
	EMAGE:21073 same embryo
	EMAGE:21074 same embryo
	EMAGE:21075 same embryo
	EMAGE:21077 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source