EMAGE mouse gene expression spatial database

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EMAGE:21121

Reg3b regenerating islet-derived 3 beta ( MGI:97478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005547. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005547_01	euxassay_005547_02	euxassay_005547_03	euxassay_005547_04

euxassay_005547_05	euxassay_005547_06	euxassay_005547_07	euxassay_005547_08	euxassay_005547_09

euxassay_005547_10	euxassay_005547_11	euxassay_005547_12	euxassay_005547_13	euxassay_005547_14

euxassay_005547_15	euxassay_005547_16	euxassay_005547_17	euxassay_005547_18	euxassay_005547_19

euxassay_005547_20	euxassay_005547_21	euxassay_005547_22	euxassay_005547_23	euxassay_005547_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21121_wholemount_strong.wlz

21121_wholemount_moderate.wlz

21121_wholemount_weak.wlz

21121_wholemount_possible.wlz

21121_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21121_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

strong

single cell

strong expression: see section 3 4 5 6 7 7 8 9 0

ventral grey horn

strong

single cell

strong expression: see section 7 8 9 1 2 3 4 5 6

not examined

single cell

not examined expression: see section 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3063
Entity Detected:	Reg3b, regenerating islet-derived 3 beta ( MGI:97478)
Sequence:	sense strand is shown >T3063 TGNCCTCGAGGCAGATTCGTCGACTCCTCTGGAAGACAGACAAGATGCTGCCTCCAACAGCCTGCTCCGT CATGTCCTGGATGCTGCTCTCCTGCCTGATGCTCTTATCTCAGGTTCAAGGTGAAGACTCCCTGAAGAAT ATACCCTCCGCACGCATTAGTTGCCCCAAGGGCTCCCAGGCTTATGGCTCCTACTGCTATGCCTTGTTTC AGATACCACAGACCTGGTTTGATGCAGAACTGGCCTGCCAAAAGAGGCCTGGAGGACACCTCGTATCTGT GCTCAATAGCGCTGAGGCTTCATTCTTGTCCTCCATGGTGAAGAGAACAGGAAACAGCTACCAATACACT TGGATTGGGCTCCATGACCCCACTCTGGGTGCAGAACCCAATGGAGGTGGATGGGAATGGAGTAACAATG ACGTGATGAATTACTTTAACTGGGAGAGGAACCCATCTACTGCCTTAGACCGTGCTTTCTGTGGCAGCTT GTCAAGAGCTTCTGGATTTCTAAAATGGAGAGATATGACATGTGAGGTGAAGT
Notes:	The probe template was PCR amplified from IMAGE:1546513 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546513 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005547 same experiment
	EMAGE:21122 same embryo
	EMAGE:21123 same embryo
	EMAGE:21124 same embryo
	EMAGE:21125 same embryo
	EMAGE:21126 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source