EMAGE mouse gene expression spatial database

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EMAGE:21231

E130008D07Rik RIKEN cDNA E130008D07 gene ( MGI:3584523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014152. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014152_01	euxassay_014152_02	euxassay_014152_03	euxassay_014152_04

euxassay_014152_05	euxassay_014152_06	euxassay_014152_07	euxassay_014152_08	euxassay_014152_09

euxassay_014152_10	euxassay_014152_11	euxassay_014152_12	euxassay_014152_13	euxassay_014152_14

euxassay_014152_15	euxassay_014152_16	euxassay_014152_17	euxassay_014152_18	euxassay_014152_19

euxassay_014152_20	euxassay_014152_21	euxassay_014152_22	euxassay_014152_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21231_wholemount_strong.wlz

21231_wholemount_moderate.wlz

21231_wholemount_weak.wlz

21231_wholemount_possible.wlz

21231_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21231_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 5 6 7 8 9 0 1 2 3 4 5 6

telencephalon mantle layer

weak

regional

weak expression: see section 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 5 6 7 8 9 0 1 2 3 4 5

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 5 6 7 8 9 0 1 2 3 4 5

rest of cerebellum mantle layer

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 7 8

pons mantle layer

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

midbrain mantle layer

weak

regional

weak expression: see section 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40130
Entity Detected:	E130008D07Rik, RIKEN cDNA E130008D07 gene ( MGI:3584523)
Sequence:	sense strand is shown >T40130 AGAAAACAGACTCTGGGACAGCTGTGTACATAAGAGTCATCTGGGCATTGCTCTTACAATCCCTGTAGAA GACAAGAGCTCAGATTGACCCACCCGAAGTCCCTGGGATTCACTTCTTCACCCTTCACACCTCGGCTTGC AGAAACAAGATGCCACGTTTGACTTTATGTGGTTGCTGGGAACATGAATTTGGGTCCTAGGGTTTACAAG TCAAGCACTTTACCCATTGACCATCTCCCTAGCCGTGTGACTGGCTTCTTTACCTTCATGATAACTACTC ATCAGCTATATTTTAATCTGGCAAGGGGACTTGAATTTGAATGAGATGACTCTAGTTAAATAGTTTTGAT GAGGAGTGACAACTGAAGGCTCTTCGGGGCTAAAGGGATACTTAGGTGTTGTGTTCCATCCCCACCCCTG TTGACTATATAGGGCGATACAATGACTAACACAGGCTGGACACTTTAGAAGACAAACTTCAGACAATTCC CCACCAAAAGGATGTATACCCTGGTTATGGGTTTTTGGTTTGGGAGACTATGTATGCTAAGATGAATGTA GCTTGATGAGTTGGGAAGACACTACATATGAGATGAGCCATGGTAAAGACTGCATAAAGACAGGGGCAGA CTTGGATCTTTCCTTTCTCGGTTGCTAAATGACTGAAAAAGCCTTTTAACCCCATAGACCTTCTGCTCAT CTAAACAGAGTGGAGGTTGGCACTATCTAAGACATATAAAGAACCGTCAAAACTCAGTGATTAAAATATT AGACTAGGAAACTTAGAAAAGACAGATCTGCCACTAAAGAGGCTATAAAGATGACAAAGTAGTATATGAA AAGAGGATCAACAGCATAAGCCATCAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 269597. Forward Primer - name:269597_F_cDNA_E130008D07Rik, sequence:AGAAAACAGACTCTGGGACAGC; Reverse Primer - name:269597_N_SP6_cDNA_E130008D07Rik, sequence:ATGATGGCTTATGCTGTTGATCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_014152 same experiment
	EMAGE:21229 same embryo
	EMAGE:21230 same embryo
	EMAGE:21232 same embryo
	EMAGE:21233 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source