EMAGE mouse gene expression spatial database

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EMAGE:21347

Slc35c1 solute carrier family 35, member C1 ( MGI:2443301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000253_01	euxassay_000253_02	euxassay_000253_03	euxassay_000253_04	euxassay_000253_05

euxassay_000253_06	euxassay_000253_07	euxassay_000253_08	euxassay_000253_09	euxassay_000253_10

euxassay_000253_11	euxassay_000253_12	euxassay_000253_13	euxassay_000253_14	euxassay_000253_15

euxassay_000253_16	euxassay_000253_17	euxassay_000253_18	euxassay_000253_19	euxassay_000253_20

euxassay_000253_21	euxassay_000253_22	euxassay_000253_23	euxassay_000253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21347_wholemount_strong.wlz

21347_wholemount_moderate.wlz

21347_wholemount_weak.wlz

21347_wholemount_possible.wlz

21347_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21347_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2220
Entity Detected:	Slc35c1, solute carrier family 35, member C1 ( MGI:2443301)
Sequence:	sense strand is shown >T2220 CTGAGCCTCTCATGAAGGTAGGCTTATGTTCTCTGAGGCTGGGGCCGGAGGAGCGCACTGATTCTCGGAG TTATCCCATCAGGCTCCTGTCACAAAATAGCCTAGGCCGTGTGTCTAAGAACAGTGGAGGTTGGCTTATA ACTGTTCTGGGGGCAGCGAAGCCCACATCAAGGTACTCATAGACCCAGTATTTCTGAGGAAACCCCTGTC CACATCCTCACTTGGTAAAGGCACAGATAATCTCCCTCAGGCCTCTTGTATAGGAGCACTAGCCCTGGGA GGGCTCCGCCCCATGACCTGATCACCCCAAAGCCTTCAACAGAAGGATTCCAACATGAATTTGGGGACAG AAGCACTCAGACCACGATGCCCAGCACCACACCCTCCTATCCTCAGGGTAGCTGTCACTGTCCTAGTCCC TTCTGTTTGGCCTTTTGTACCCTCATTTCCTTGGCGTCATGTTTGATGTCTTTGTCTCTTTCGTGAGAAG ATGGGGAAACCATGTCAGCCTCTGCTTCCGACTTCCCATGGGTTCTAATGAAGTTGGTGGGG
Notes:	The probe template was PCR amplified from IMAGE:949450 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:949450 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000253 same experiment
	EMAGE:21350 same embryo
	EMAGE:21349 same embryo
	EMAGE:21348 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source