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EMAGE:21447

Prpf18 PRP18 pre-mRNA processing factor 18 homolog (yeast) ( MGI:1914479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447
euxassay_000886_01 euxassay_000886_02 euxassay_000886_03 euxassay_000886_04 euxassay_000886_05
EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447
euxassay_000886_06 euxassay_000886_07 euxassay_000886_08 euxassay_000886_09 euxassay_000886_10
EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447
euxassay_000886_11 euxassay_000886_12 euxassay_000886_13 euxassay_000886_14 euxassay_000886_15
EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447
euxassay_000886_16 euxassay_000886_17 euxassay_000886_18 euxassay_000886_19 euxassay_000886_20
EMAGE:21447 EMAGE:21447 EMAGE:21447 EMAGE:21447
euxassay_000886_21 euxassay_000886_22 euxassay_000886_23 euxassay_000886_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21447Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21447_wholemount_strong.wlz
21447_wholemount_moderate.wlz
21447_wholemount_weak.wlz
21447_wholemount_possible.wlz
21447_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21447_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
oral region
moderate moderate
regionalmoderate expression: see section 7
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 3 4 5 6 7
vibrissa
weak weak
regionalweak expression: see section 7 8 9 2 3
medulla oblongata basal plate
moderate moderate
homogeneousmoderate expression: see section 7 9 3 6 7 weak expression: see section 6 8
facial vii ganglion
moderate moderate
homogeneousmoderate expression: see section 3 4 5 6 7 0 1 2
inferior glossopharyngeal ix ganglion
moderate moderate
homogeneousmoderate expression: see section 6 7 9
superior glossopharyngeal ix ganglion
moderate moderate
homogeneousmoderate expression: see section 6 7 9
trigeminal v ganglion
moderate moderate
homogeneousmoderate expression: see section 3 4 5 6 7 8 9 0 1 2 weak expression: see section 8 9 7
vagus x ganglion
moderate moderate
homogeneousmoderate expression: see section 8 weak expression: see section 8 9 7
vestibulocochlear viii ganglion cochlear component
moderate moderate
homogeneousmoderate expression: see section 6 7 8 9
vestibulocochlear viii ganglion vestibular component
moderate moderate
homogeneousmoderate expression: see section 6 7 8 9
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 7 8 weak expression: see section 8 9 0 1 2 3
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 0 1 2 3 5 6 7 8 9 0
hindgut
moderate moderate
regionalmoderate expression: see section 3 4 5 6
midgut
moderate moderate
regionalmoderate expression: see section 8 9 0 1 2 3 4 5 6 8 9 0 1
not examined not examined
regionalnot examined expression: see section 8 9 0 1 2 3 4 5 6 7 8 9 0 1
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T981
Entity Detected:Prpf18, PRP18 pre-mRNA processing factor 18 homolog (yeast) ( MGI:1914479)
Sequence:sense strand is shown

>T981
TCCTCGAGCCTGTTGGCCTACTGGAGATTTCCGCCGGAAGCGGCTCCGGCAGTTGTTGTTTTCCGATAAT
TGGGCTCTGGTTGCTTCGTGGTTTGTCCTCTTCCCCCTCCCCTGGCGGGTGAGGTTACCGCCGAGGAGCT
GGGGAGTCAAAGTGAAGACACTTAGACCCTGGGAGCGAGATGGACATCCTGAAATCCGAGATTCTGCGGA
AGCGGCAGCTGGTGGAGGACAGGAACCTGCTGGTGGAAAATAAAAAATACTTCAAGCGGAGTGAACTGGC
GAGAAAAGAAGAGGAAGCGTATTACGAAAGATGTGGCTACAAGATACAGCCAAAAGAGGATGACCAGAAA
CCACTAACATCGTCAAATCCAGTGCTGGAACTTGAACTAGCAGAGGAAAAACTTCCTATGACTCTTTCTA
GGCAAGAGGTCATCAGAAGACTGAGAGAAAGAGGAGAGCCGATTAGACTGTTTGGGGAAACCGACTATGA
TGCTTTCCAGCGTTTAAGGAAGATAGAGATTCTTACACCAGAAGTTAACAAGGGCTTAAG
Notes:The probe template was PCR amplified from IMAGE:1972395 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972395 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000886 same experiment
 EMAGE:21449 same embryo
 EMAGE:21445 same embryo
 EMAGE:21446 same embryo
 EMAGE:21448 same embryo
 EMAGE:21444 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS