EMAGE mouse gene expression spatial database

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EMAGE:21471

Ascc2 activating signal cointegrator 1 complex subunit 2 ( MGI:1922702)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002894_01	euxassay_002894_02	euxassay_002894_03	euxassay_002894_04	euxassay_002894_05

euxassay_002894_06	euxassay_002894_07	euxassay_002894_08	euxassay_002894_09	euxassay_002894_10

euxassay_002894_11	euxassay_002894_12	euxassay_002894_13	euxassay_002894_14	euxassay_002894_15

euxassay_002894_16	euxassay_002894_17	euxassay_002894_18	euxassay_002894_19	euxassay_002894_20

euxassay_002894_21	euxassay_002894_22	euxassay_002894_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21471_wholemount_strong.wlz

21471_wholemount_moderate.wlz

21471_wholemount_weak.wlz

21471_wholemount_possible.wlz

21471_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21471_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 1 2 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1585
Entity Detected:	Ascc2, activating signal cointegrator 1 complex subunit 2 ( MGI:1922702)
Sequence:	sense strand is shown >T1585 ATCGGCTGGCCCCTGAGCTCAGTCAGCTGGACCGAGGCCTAGAAAGACAAGTGAAGCCGGACCCTACACC CCTGTTGTCATCTCGTCACAACATCTTCCAGAACGATGAGTTTGATGTGTTCAGCAGGGACTCGGTGGAC CTGAGCCGAGTGCACAAGGGCAGGAGGAAGGAGGAGAACGTGAGGAGCCTGGTGAATGACAAGCAGGCTG TGGTGGCACAGTGGCAGCGCTACCAGAAGTACAGCGTGGTGGTAGAGGAGGTCCCCCTGCAGCCAGGAGA ATACCAGGCTGATGACTATGAGGACGAGTATGACGACACATACGATGGCAACCAGGTGGGCGCCAACGAT GCAGACTCTGATGACGAGCTCATCAGCCGCAGGCCCTTCACCATCCCTCAGGTGCTGAGAACCAAAATGC CTGGCGAAGTGCAGGAGGAGGAGTGGGATGAAGAGGATGAGGTGGAAGAGGAGGCCCCCAAGCCAGACCA TTTCATCCAGGATCCTGCAGTGCTGAGGG
Notes:	The probe template was PCR amplified from IMAGE:945686 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:945686 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002894 same experiment
	EMAGE:21473 same embryo
	EMAGE:21472 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source