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EMAGE:21692

Hcrtr2 hypocretin (orexin) receptor 2 ( MGI:2680765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692
"Pseudo-wholemount" of euxassay_018731. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018731_01 euxassay_018731_02 euxassay_018731_03 euxassay_018731_04
EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692
euxassay_018731_05 euxassay_018731_06 euxassay_018731_07 euxassay_018731_08 euxassay_018731_09
EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692
euxassay_018731_10 euxassay_018731_11 euxassay_018731_12 euxassay_018731_13 euxassay_018731_14
EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692
euxassay_018731_15 euxassay_018731_16 euxassay_018731_17 euxassay_018731_18 euxassay_018731_19
EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692 EMAGE:21692
euxassay_018731_20 euxassay_018731_21 euxassay_018731_22 euxassay_018731_23 euxassay_018731_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21692Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21692_wholemount_strong.wlz
21692_wholemount_moderate.wlz
21692_wholemount_weak.wlz
21692_wholemount_possible.wlz
21692_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21692_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50518
Entity Detected:Hcrtr2, hypocretin (orexin) receptor 2 ( MGI:2680765)
Sequence:sense strand is shown

>T50518
GATGTGCTTGTGACCATCACCTGCCTTCCAGCTACCCTCGTTGTTGACATCACTGAGACTTGGTTCTTTG
GACAGTCCCTCTGTAAGGTCATTCCTTATTTACAGACGGTGTCAGTGTCTGTGTCTGTTCTTACGTTGAG
CTGCATTGCCTTGGACCGATGGTACGCCATTTGTCACCCTTTGATGTTCAAGAGCACAGCCAAACGGGCT
CGAAACAGCATCGTTGTCATCTGGATTGTCTCCTGCATCATAATGATTCCTCAAGCCATTGTCATGGAGT
GCAGCAGCATGCTCCCTGGCCTAACCAATAAGACCACCCTCTTTACAGTATGTGATGAACACTGGGGCGG
TGAAGTTTACCCAAAGATGTACCATATCTGCTTCTTTCTGGTGACATACATGGCACCTCTGTGTCTTATG
GTATTGGCTTATCTCCAAATATTCCGTAAACTCTGGTGCCGACAGATTCCCGGAACTTCTTCTGTGGTTC
AGAGAAAATGGAAGCAGCAGCAGCCGGTTTCTCAGCCCCGGGGGTCCGGACAGCAGAGCAAGGCTCGGAT
TAGCGCTGTTGCTGCTGAGATAAAGCAGATCCGAGCACGAAGGAAAACAGCCCGGATGCTCATGGTTGTA
CTTCTGG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018731 same experiment
 EMAGE:21693 same embryo
 EMAGE:21691 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS