EMAGE mouse gene expression spatial database

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EMAGE:29225

Pole ( MGI:1196391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005162_01	euxassay_005162_02	euxassay_005162_03	euxassay_005162_04	euxassay_005162_05

euxassay_005162_06	euxassay_005162_07	euxassay_005162_08	euxassay_005162_09	euxassay_005162_10

euxassay_005162_11	euxassay_005162_12	euxassay_005162_13	euxassay_005162_14	euxassay_005162_15

euxassay_005162_16	euxassay_005162_17	euxassay_005162_18	euxassay_005162_19	euxassay_005162_20

euxassay_005162_21	euxassay_005162_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8829
Entity Detected:	Pole, ( MGI:1196391)
Sequence:	sense strand is shown >T8829 CGCAATGGTCCTGAGGAACAGTGGACGGAGGCACCCCGAGCCGGGCGCGGATGGCGAAGGCAGCCGGGAT GATGGTCCCTCTTCCTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACAGACAAGATGGACT TACGGTTTGGTTTCGAAAGGCTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTGATCAACATGCACCCTAC TGAGATCTTAGATGAAGACAAACGCTTAGTCAGCGCGGTGGATTACTACTTCATTCAAGATGATGGAAGC AGATTTAAGGTGGCCTTGCCCTATATGCCGTATTTCTACATTGCAGCGAGAAAGGGTTGTGATCGAGAAG TTTCATCTTTTCTATCCAAGAAGTTTCAGGGAAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGATCT GGACTTGCCAAATCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGAC CTTGTCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGATG AGTATACAACAATGCTCTCCAGTATTCTGCAAGGTGGCAGTGTAATTACTGATGAGGACGAAACCTCTAA GAAGATAGCTGACCAATTGGACAACATAGTGGACATGCGAGAGTATGATGTTCCCTACCACATTCGCCTC TCCATTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGG AAATCACCCGACGAGATGATCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATCGAGACGAC CAAACTGCCTCTCAAATTCCCTGATGCTGAGACCGATCAGATCATGATGATCTCCTATATGATTGATGGC CAGGGCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTCACCCCTAAGC CAGAATATGAAGGGCCCTTTTGTGTCTTCAATGAACCCGACGAGGTCCATCTGATCCAGAGATGGTTTGA GCATATCCAGGAAACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCATTTGTG GAGGCTAGGGCAGCAATTCATGGCCTCAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGG AATATAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGT GGGCAGTCATAATCTCAAGGCAGCTGCCAAGGCCAAACTTGGCTATGACCCTGTAGAGCTGGACCCTGAG GACATGTGTCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTGGCTA CTTACTACCTGTACATGAAATACGTCCACCCCTTCATATTCGCCCTGTGCACCATTATTCCCATGGAACC TGATGAGGTGCTGCGGAAGGGCTCCGGGACACTGTGTGAAGCCTTGCTGATGGTGCAAGCTTTCCATGCC AACATTATCTTCCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACATGCTAGATG CTGAGACCTACGTCGGGGGCCACGTGGAGGCACTAGAGTCTGGTGTCTTCAGAAGTGATATCCCCTGCCG GTTTAGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAACGAGTCGAGAAGACTATGCGCCATTCCATT GAAGAAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTTCAAGAGGTGTGTGAGCAGATTAAGACCA AGCTCACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGC CATGTATCCTAACATAATTCTTACCAACCGCCTACAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCT GCCTGTGACTTCAATAAGCCTGGAGCAAGTTGTCAGAGGAAGATGGCCTGGCAGTGGAGGGGAGAATTCA TGCCAGCCAGTCGCAGTGAATACCATCGGATTCAGCATCAGCTGGAGTCGGAGAAGTTTCCCCCTTTGTT TCCAGAGGGGCCAGCACGGGCCTTTCACGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAGAGGAGG CTGGCAGATTATTGCCGGAAAGCCTATAAGAAGATCCATGTGACCAAGGTAGAAGAACGTCTAACTACCA TCTGCCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAGTTCAA AGGACTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCAGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAG CGCTGCAAGAACATGGAGATCCTTTACGATTCACTGCAGCTGGCTCACAAGTGCATCCTGAACTCCTTCT ACGGCTATGTCATGCGCAAAGGAGCTCGCTGGTATTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGG AGCCAACATCATCACCCAAGCAAGAGAACTGATTGAGCAGATCGGGAGGCCTTTAGAATTGGACACGGAC GGAATATGGTGCGTCCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGCGAAGAAAC CCAAACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCA GTACCAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCACTCTGAGAATAGTATCTTTTTTGAA GTCGATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGAT ATGCTGTGTTCAATGAAGATGGTTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTT GCAGCTGATTAAAATATTCCAGTCCTCAGTTTTTGAGGCCTTCCTCAAGGGCAGCACACTGGAGGAAGTG TATGGCTCGGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCG ATTCTGAATTGTTTGAGCTGATTTCTGAGAACCGCTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCA GAAGTCTACATCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCT GGACTGAGCTGCCGCTATATCATCTCCCGAAAGCCAGAGGGGTCTCCTGTCACTGAGAGGGCCATTCCAC TTGCCATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGCTAAAGAGTTCATCACT TCAAGACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGAGGCTGGGGAGTGCCATCCAG AAAATCATCACCATCCCCGCAGCTCTGCAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACT GGCTACACAAAAAACTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCT TGAAGGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATG GAGGACATTGGACTCACAAAGCCACACCACTCTACAGTCCCAGTTGCTACTAAGAGGAAGCGAGTCTGGG AGACCCAAAAGGAGTCTCAGGATATTGCACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTC CCTTGGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCC CAACAGCGCCTAGCCCTCAGGAAGAAGCAACGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGCCTA TCCGAGAGGGGCCTTCCACAGGACTGGGGAGCTTTTTGCGAAGGACTGCCCGCAGCATCATGGACCTTCC ATGGCAGATAATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAAT GACTTGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTTGCCAAAGCAGAGG ATGGACCTGCATATCGGAAGGTGAATCGGGCTCTTCCTCGTTCCAACATTGTCTACAATCTCTATGAGTA TTCAGTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGTACCAGACATT GAGGGCGTGTATGAGACACAGGTCCCATTGTTATTCCGGGCCCTCGTGCAGCTGGGCTGTGTGTGTGTGG TCAACAAGCAGCTGACAAGGCACCTTTCGGGCTGGGAAGCTGAAACTTTTGCCCTCGAGCACCTTGAAAT GCGTTCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCATCACACT CAGGGCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATA CTGTACGAAGCAACCAAATGCCAGGGCTCAGTGCCCTGTACTCATCAGAACACAGCCTGCTGCTGGACAA GGTGGACCCCAAGCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACT ATCTGCAGAGCCATCCAGCGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGAGGGCCCACACTCATCGCTG TCCAGTCTAGCTGGGAGCTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAAGAGTTCCCACTAGTGCC TATCCGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCATGGAGCTCGCCGAATGATC CGGCACTACCTCAATTTAGACTTGTGCCTGTCGCAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCTG TTGGAAACCTGCCGGAAGACATCTCCACCTTTGGCTCAGACCTCTTTTTTGCACGCCACCTCCAGCACCA TAACCACCTGCTTTGGCTATCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGC CTTGTCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAGTTCTGGCTGTTACTCTACTGTGTGCG TGGAACTGGACATTCAAAATCTGGCAGTCAACACCATCCTCCAGTCCCATCATGTCAATGACATGGAGGG GGCTGGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTAACAGGCAAT CAAGCTGCCAGTGCCCTGGCCAACTACGATGAGACAGCCCTCTGCTCTAGTACCTTCAGGATCCTGAAGA GCATGGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTAATGCA CTTCTACCGCTGGCTCCAGTCACCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACGCTGCACAAC ATGATGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCAGTCGTCTATGCCA ACTTCAATCGCATCATTCTCTGTACAAAGAAGCGCCGAATAGAGGATGCCCTTGCCTATGTGGAATATAT TACCAACAGCATCCATTCTAAAGAGATCTTCCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTC CTTCTCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTTCCATCTAGTATTCACTGTGGAC AGGTAAAAGAGCAAGACTCCCAGGCAAGAGAGGAAACTGATGAAGAGGAGGAGGACAAGGAAAAGGACGA GGAGGAAGAGGGCATGGGAGAGTCCGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTC TTGCCCCAGGCAGCCTCTTGCCAGAGCTACTTCCTCATGATTGTTTCAGCATACATCGTAGCTGTGTACC AAAGCATGAAGGAGGAGTTGAGACACAGTGCCCCGGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCA GTTCTCCCAGGAGTCTGAAGGGGCAACTGGATCTCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTG GCAAATGAGCTCACTCAGAGCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGTTCTCGGA ACACCACTGAGCCCTCAGAGATGTTCCCCGTCCTCCCTGGTTCACACTTGCTGCTCAATAATCCTGCTCT GGAGTTCATCAAATATGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTG AACAGAGACCTTCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCC ACTCCTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTGTGCAAAGA TTCCTCTTTCTCTCAGGATGGAGCCATCCTGCCTCAGTGGCTCTGCTCCAATTGTCAAGCCCCCTATGAC TCCTCTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACC TGGTATGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCAGCTGCGCAGGGGACTT TACTCTCACCATCCGCACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTAC AGCATGTCATATCTCCAGGAGACCATAGAATGGCTGTTACAGACAAGCCCTGTATCAAACTGTTAGCAAG CCTAGGCTAAAGACACTTTGGTATCCCACACCTACTGCCTGCTCCAAAAGGCAGAACCACTGACCACCTT GCTTTTCCAAACTCATGAGCACAGCCCAGAAGGAACAGAAGACTTCTGCTAACGTCATCATGCCATAAAC AGACAGAAGCAGGGAATGGCTCTATCCCTAGCTGCCTGCTAAGTAAACACGGTTTTGAAGCGTCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410453 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410453 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005162 same experiment
	EMAGE:29196 same embryo
	EMAGE:29244 same embryo
	EMAGE:31123 same embryo
	EMAGE:29246 same embryo
	EMAGE:29227 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source