EMAGE mouse gene expression spatial database

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EMAGE:29244

BC023151 ( MGI:2444047)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005142_01	euxassay_005142_02	euxassay_005142_03	euxassay_005142_04	euxassay_005142_05

euxassay_005142_06	euxassay_005142_07	euxassay_005142_08	euxassay_005142_09	euxassay_005142_10

euxassay_005142_11	euxassay_005142_12	euxassay_005142_13	euxassay_005142_14	euxassay_005142_15

euxassay_005142_16	euxassay_005142_17	euxassay_005142_18	euxassay_005142_19	euxassay_005142_20

euxassay_005142_21	euxassay_005142_22	euxassay_005142_23	euxassay_005142_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8612
Entity Detected:	BC023151, ( MGI:2444047)
Sequence:	sense strand is shown >T8612 CCCGCACTGAGTGGAGAGGGCAGCGGCCGTCTGGGCTGAGGCAGCTTTCCATGGACGGCTCTGAAGATGA CCCGACCATATTCAGTGCCCGTTGTCTGCCCAGTGACCCCAGGCTCTGGGCCACTGTAACGAACTCATAC CTAGGTACACGTGTGTATCATGACACCATCCATATAAACGGTGTGTACAATGGAGCTGTCGGGGACACTC ACCGGGCCAGTCTGCCCAGCCCCCTCAATGTCCAGCTGGAGGCCCCTGCAGGGACAGAACAGCTGACAGA GACCTTTACCCTGGACACCAACACAGGTTCCTTCCTCCACACCCTGGAAGGCCCCAGCTTCCGGGCTTCC CAGCGGATCTATGCTCACCGTGTGTTACCCCATGTCCTGGTCTTCAGTGTGTCCATTGCACGCTTGACCA CAGGGAACAAACCCATCACAGTGCCGTTACGGGCAGACTTCTCCCCCGAAAGCCCAGACCTGGACCTGCG TGTGGGGCCTGACTTCCAGGGACTCCGGTACCTGCATGGCCACGTACTTAACCCTGAGCAGCCTGGAGAG CCACAGCAGGAAGTGCATATGCTATGGATGCCTGTCCCCCCAGCCTTGACCCTTGGGGAAGAGGAGAAGG ACAGGACCTGGGAGTTTCTGACTGTTGTGGGTAGCAGCCAGGCTGAAGCCCAGGACTGCTTTGCTGAGGC CCTACAGCTGCAGACCAGGGGTGTTCTGTATACAATCCATGCAGACTCCTGGGGCCGGCTCTGGGCAGGC TGTGGCCTGGATGTGGCTGGGCCCCTGGCCCTGCGCCAGGCCCTTCGTGGCTCTCTCTACTACCTGTTCA GTGAGCTTCCCCAGCCTGGCACTCAAGGATTCATCAGCCATGGGCTTAGCCCCGGAGGACTCTCCAATGG GAGTAAGGAGGAATGCTACTGGGGCCATATCTTCTGGGATCAGGACATCTGGATGTTCCCAAATATTTTG ATGTTCCACCCAGAAGCCGCCAGGGCCATCCTAGAATACCGAGTCCGTACGCTGGGAGGAGCCCTGAAGA ATGGCCAGAACCTGGGCTACCAGGGAGCCAAGTTTGCTTGGGAGAGTGCAAGCACTGGCCTGGAGGTCTG CCCTGAGGACATTTATGGGACCCAGGAGATCCATATCAATGGGGCTGTGGCCTTGGCCTTTCAGCTGTAT TACTATTACACTCAGGACTCGAAGCTCTTCCAAGAGGATGGTGGCTGGGATGTGGTCAGTTCTGTGGCTG AGTTTTGGTGTAGCCGTGTAGAATGGAGCTCCCAGGACAAGATGTACCACCTGAAAGGAGTCATGCCCCC CGATGAGTACCATTCAGGAGTCAACAACTCTGTCTACACCAATGTCCTGGTCCAGAACAGCCTGCACTTC GCTGCTGCTTTGGCCAAGGACTTGGGTTTGCCTATCCGAAAACAGTGGCTGGAGGTGGCTGATAGGATCA AGATACCATTTGACTCTGAGCAGAACTTCCACCCTGAGTTTGATGGCTATGAGCGTGGAGAAGAGGTGAA GCAGGCAGATGTTGTGCTCCTGGGGTACCCAGTCCCCTTTCCCCTGACACCTGACATCCGAAGGAAAAAC TTGGAGACTTATGAGGCCGTGACATCCCCCCAGGGCCCTGCTATGACCTGGAGCATGTTTGCTGTGGGCT GGATGGAACTGAGGGACCCCTCAAGGGCTCAGGTCCACCTGAGCAGGAGTTTCGCCAATGTCACTGAGCC CTTCAAGGTGTGGACGGAGAATGCAGACGGGTCTGGTGCAGTGAACTTCTTGACTGGCATGGGGGGCTTC CTGCAGGCAGCACTCTTCGGGTGCACAGGATTCAGGATCACTGAGGCAGGTGTGACCTTTGACCCCCTGT GTCCAGACCTGGTCTCCAGAGTATCTGTCTCTGGTATCTCCTACCTGGGGAACAAGATCAACTTCGCCTT TTCCAAGGACTCGGTGACACTTGAGGTCACAGCCCGTGCAGAGCCCTGGGCACCCCTACTAGAAGCTGAG CTGTGGCCATCGCTGGCTCATCTTCCCCTGACACCAGGACAGAAGGTCTCCTTTCCCCACTCAGCTGGCC GGATACAAAGGTCATCCCCATAGCTGCCCAGAAATTCTTCCGTGGTTCCTGATAGGAGTTTTGGCATTAG AAGTCCCTTTCAGTTCCTCTGGGACACTCTTTTGCCCTCAACTGCATCCCCAACCTCACAACCTCTTAGT CTGGATCCAGCCCTGAAGCAGTAAGGTTACAGGGTGTCCCTTCTGTCCCTTCTGTCCCCTCCCTCCTTTC CTCACCCTCGCCAGTCCTGGGCTCTCTAGGCCTCACAGATCCTTTGTTTGGGGCTGACCGCAGCACCCTC CCCTCCTTATCTCCCCACCTTCCTGTCTCTCTAGGCCTTAACTTCCTGTTCACACGTCTGTTCTCTGGGA AACTCTGGTCAGCAGGCTAGGTGGGAAGTGGCCAGTGACTCCGCTGAGAAGTGAGTCACAGTCAGGGGCT TAGCTACAGGAGTTTCTTGTGGCAGGCAGTTGACACCAGGTAGGGCTAGCCAAGTGCTCAGAGGTTTTGC GGCTTTGAACTTCCTAGTCTTTGATTCCTTTCTGACGTCAGATGGTATCTAGCGTACTGGGCTAAGGTGA GAGCTGTCCTTAACCACTCAGCCCGTGAGAAGGAAGGCAGTGTTATCACAGGACAGGGTAAATGAAGTCA GACTGGCGACCAGAGGGAATGGAGTCAGATGGACAGGGTTCCAGGCCATGGTCCTGGCCATCAGTGCTGA GCAGCAGCGTGGCCTTCACTTCCATTCTTAGTGTTTCTCCAGAACAAGGTTCATAGCGTAGGACCACGAT CCTGGTAAACCCTGTGCTCCTGTACCAAGCCAACTTGGCTAATTAAAGTCAGTGCTGGCAAAGGCTGAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5717810 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5717810 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005142 same experiment
	EMAGE:29196 same embryo
	EMAGE:29225 same embryo
	EMAGE:31123 same embryo
	EMAGE:29246 same embryo
	EMAGE:29227 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source