EMAGE mouse gene expression spatial database

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EMAGE:29246

D530033C11Rik ( MGI:1925831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005144_11	euxassay_005144_12	euxassay_005144_13	euxassay_005144_14	euxassay_005144_15

euxassay_005144_16	euxassay_005144_17	euxassay_005144_18	euxassay_005144_19	euxassay_005144_20

euxassay_005144_21	euxassay_005144_22	euxassay_005144_23	euxassay_005144_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8611
Entity Detected:	D530033C11Rik, ( MGI:1925831)
Sequence:	sense strand is shown >T8611 CCGGTGAGTCCGGTGGCTTAAACAGGCATGAAGATCACCAGGCAGAAACACGCCAAGAAGCATCTCGGCT TTTTCCGCAACAATTTCGGAGTCCGCGAGCCCTACCAGATCCTTCTGGATGGCACTTTCTGCCAGGCGGC GCTGCGGGGTCGCATCCAGCTGCGGGACCAGCTGCCCCGCTACCTTATGGGAGAGACCCAGCTGTGCACC ACGAGATGTGTGTTAAAGGAGTTAGAAACACTGGGAAAGGAATTATATGGAGCAAAACTGATTGCACAGA AATGCCAGGTTCGAAACTGTCCTCATTTCAAGAGCCCAGTGAGTGGGTCTGAATGTCTCCTGTCCATGGT GGATGAGGGGAACCCTCATCATTATTTTGTGGCCACACAGGATCAGAACTTATCTGTGAAAGTCAAGAGG ACTCCTGGAATTCCTCTCATGTTCATTATTCAGAACACTATAGTGTTGGACAAGCCTTCTCCCAGAACAG TGGCCTTTGTGAAGGCGGTCGAGGCGGGTCAGCTGGTCTCAGTGCACGAGAAACAGAGTATCAAGCAACT GAAGGAGGAGCAAGGCCTGGTGCGGAACCCGGACCTGAGGAGGAGGAGGAGGAAAAAGAAGAAGGTGGGC GGCCCCAATCCTCTCAGCTGCCTGAAGAAAAAGAAGAAAGCACAGGATACAAAGTCCCCTGCTTCCGAAA AGAAAAGGAAAAGGAAACGGATTCGGAACAGATCCACCCTGAAAGTGTCTTCTGAGCAGCAGGGTGCAGA AGGCTGATGTGTGCTGGGGATACTCTGGAAGACAGTGGAAACTAACTTCCTGTTCAAACTGTGAAAATGT GTATACCAGAGGACTAACTTATTTTTGTAAACTACTACTGCTACATACACTTTTGCCAAAAGTAAAGAAT AGTTGGGTTTGTCAGTGTGCATCTTCAGTTGTTGCTTTGGGGATGTATTTTTATTATAAAATTATCTCCA GTTTCTCCCCTTCTCACCCTCCAAGTGCTGTGTATGTGTGTGGTATATGAGTGGTATGTGTTTGTGAGCC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTAGGTGCAGTAGTGTTTGTGAGCTTGTTGG GCGGTGGGGGGGGGGGGTGAGCGTGTGTATGAAGCAGCTAGAGGTCAGCATGAAGTGTCTTTCTCTATCA CTCTCTACCATTATTTTTTGAGAGAGTTTCTCACTGAATCAATAACTTGCCAGTTAAGTGGTCCTGGATG GCCGTTAAGCCCCAGAGATCCTCCTGTCTCCTTTCTGGTGCTGGGATTGACAGCATTCACTGTTGAATGT GGCTTTCTGTATGAGTCTGAGGATCTGAACCCAACTCCTCACAGTAGTACGGCAGGTAAGGTCCTGACTG AGGCATCACCCCAGCACCACATAATTTTAAAAGTCAGTCCTAAGGCCAGTGAGATGACTCAGTGGGTGTC TTAGTCAGGGTTTCTATTCCTGCACAAACATCATGACCAAGAAGCAAGTTGGGGAGGAAAGGGTTTATTC TGCTTACACTTCCACATTGCTGTTCATCACCAAAGGAAATCAGGACTGGAACTCAAGCAGGTCAGGAAGC AGGAGCTGATGCAGAGGCCATGGAGGGATGTTCTTTACTGACTTGCCTCCCCTGGCTTGCTCAACTTGCT CTCTTATAGAACCCAAGACTACCAGCCCAGGGATGGCACAACCCACAAGGGGCCTTTCCCCCTTGATCAC TAATTGAGAAAATGCCTTGCAGTTGGATCTCTTGGAGGCATTTCCTCAACTGAAGCTCCTTTCTCTGTGA TAACTCCAGCCTGTGTCAAGTTGACACACAAAACCAGCCGGTACAGTGGGTAAAGACGCCTGCTGCCTAC CTTGATAACTGAACCTCGTCCCTGAGACCTAGAGACCAGACCTGACTCCCAAAATTGTCTTCTTACCTCC ACACCTCCACGCAAAACAAATTAATGTCATTTGTAAAAGTCAGTTCTAAGGGAGGAAAGGGGGATAGCTT ACCCATTATTTTGTATATGGCAATAAAATCTGTAAGAGCTTAAGATAAAAGTTGTATCTTTGACAGTTGG AGAAACCTCCACATTCAACTAACTGTTGAATACAATGCTACCGTCTGCTCTCAATAGCCTGGAAAGGATG GCTATAGCACCTTACATTTTCCTAATAAACAAGTGTTGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5717427 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5717427 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005144 same experiment
	EMAGE:29196 same embryo
	EMAGE:29244 same embryo
	EMAGE:29225 same embryo
	EMAGE:31123 same embryo
	EMAGE:29227 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source