Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29330

2810430M08Rik ( MGI:1914473)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330
euxassay_000761_01 euxassay_000761_02 euxassay_000761_03 euxassay_000761_04 euxassay_000761_05
EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330
euxassay_000761_06 euxassay_000761_07 euxassay_000761_08 euxassay_000761_09 euxassay_000761_10
EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330
euxassay_000761_11 euxassay_000761_12 euxassay_000761_13 euxassay_000761_14 euxassay_000761_15
EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330
euxassay_000761_16 euxassay_000761_17 euxassay_000761_18 euxassay_000761_19 euxassay_000761_20
EMAGE:29330 EMAGE:29330 EMAGE:29330 EMAGE:29330
euxassay_000761_21 euxassay_000761_22 euxassay_000761_23 euxassay_000761_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T144
Entity Detected:2810430M08Rik, ( MGI:1914473)
Sequence:sense strand is shown

>T144
GCCAAGATGGCTGCGGCCGTTCAGGACTCACGTGTGAGCCCAGGGGAGATACTGAAGCGTTCTCCGAAGA
AGAAGAAAAAGATGAAGATGGTAGCCAAGGCTGCAGCGTCGAAGCTAGAAGATGAGGTCAAAGACTCTTC
TGATGGAGAAGGAAGCTGTGACTCAGAGATGGACCACTCAGATGATGGAGCTGCAGAAGCAGACAGTGAG
GACAATGTTGAATCCTGTGAGGAAGACAATGAAGACGCCGTAGAGTCGAGTGCTGGGACCAATTCAGGCT
GGGCAGATGCCATGGCAAAAATCCTTAACAAAAAGACTCCTAAAAGCAAAGCCACCATCCTCACCAAAAA
CAAACAGCTGGAGAAGGAGAAGGAGAAGCTAAAGCAGGAGAGGCTGGAGAAAAGGAAGCAGCTTGATAAG
AAGCGGGAGTGGGAAATGCTGTGCAGAGTGAAGCCAGATGTTGTCAAAGACAAAGAGGCAGAGAGGAACC
TTCAGAGGATTGCGACAAGGGGTGT
Notes:The probe template was PCR amplified from IMAGE:2609663 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2609663 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000761 same experiment
 EMAGE:29350 same embryo
 EMAGE:29354 same embryo
 EMAGE:29346 same embryo
 EMAGE:30265 same embryo
 EMAGE:30210 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS