EMAGE mouse gene expression spatial database

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EMAGE:29471

Phldb1 pleckstrin homology-like domain, family B, member 1 ( MGI:2143230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008603_01	euxassay_008603_02	euxassay_008603_03	euxassay_008603_04	euxassay_008603_05

euxassay_008603_06	euxassay_008603_07	euxassay_008603_08	euxassay_008603_09	euxassay_008603_10

euxassay_008603_11	euxassay_008603_12	euxassay_008603_13	euxassay_008603_14	euxassay_008603_15

euxassay_008603_16	euxassay_008603_17	euxassay_008603_18	euxassay_008603_19	euxassay_008603_20

euxassay_008603_21	euxassay_008603_22	euxassay_008603_23	euxassay_008603_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9300
Entity Detected:	Phldb1, pleckstrin homology-like domain, family B, member 1 ( MGI:2143230)
Sequence:	sense strand is shown >T9300 TCTTCGCCAGCCTGAGGCCGCCGCGGCCCACTTGGGCAAAGGGGCCGGTGTCCCCCCAACTCCCCACAAG GAGTTCTGGCGCCCTTGGATCATGGATCCTCTCAATAGGAGCCAGCTAGGCCCTGGATGCAAGACCCAGG CTGTAGTGCAGAAAGGGCCATTGGATCTGATTGAGACGGGTCAAGGACTGAAGGTGCAGACGGACAAACC TCATCTGGTGAGCCTGGGCAGCGGGAGACTCAGCACAGCGATCACTCTCCTGCCGCTGGAGGAAGGGAGG ACAGTCATTGGTTCTGCAGCCAGGGACATCTCACTGCAGGGTCCAGGCCTGGCTCCAGAACACTGCTACA TCGAGAATCTGCGGGGCACTCTCACCCTGTACCCTTGTGGCAATGCCTGCACTATTGATGGGCTCCCTGT TCGGCAGCCCACCCGGCTCACTCAGGGCTGCATGTTGTGTCTGGGTCAATCCACCTTCCTGCGCTTTAAC CATCCAGCCGAAGCCAAGTGGATGAAAAGTATGATTCCAGCAGGGGTCCGGGCCCCAGGGCCCACCTACA ACCCTGGCTCTGCTGAATCGGAGAGCCTGGTCAATGGGAACCACACAGCACAACCTGCAACCCGGGCACC GTCAGCCTGTGCCAGCCACAGTTCCCTGGTGAGCTCTATTGAAAAGGACCTGCAGGAAATCATGGATTCA TTGGTGCTAGAGGAGCCTGGAGCTGCAGGCAAGAAGCCTGCTGCCACATCCCCATTGTCGCCAATGGCTA ACGGCGGGCGCTACCTGTTGTCCCCTCCAACCAGCCCCGGTGCCATGTCTGTGGGCTCCAGCTATGAGAA CACCTCTCCAGCCTTCTCTCCACTTTCCTCACCAGCCAGCAGCGGAAGCTGTGCTAGCCATTCACCAAGT GGGCAGGAGCCAGGACCCTCTGTGCCCCCATTGGTGCCTGCCCGCTCCTCTAGCTACCATTTGGCTCTGC AACCTCCACAGTCTCGCCCAAGTGGTTCCCGCTCCTCTGACAGTCCCCGGCTGGGCAGAAAAGGGGGTCA TGAGAGACCTCCCAGCCCTGGTCTCCGGGGTCTTCTGACGGACAGTCCTGCCGCTACCGTCTTGGCTGAG GCCCGCAGAACCACTGAGAGTCCCCGGCTAGGTGGGCAGTTACCTGTGGTGGCTATCAGCCTGAGTGAAT ACCCATCTTCCGGTGCCCGCAGCCAACCCGCCAGTATTCCTGGCAGCCCCAAGTTCCAGTCTCCAGTGCC TGCTCCCCGAAACAAGATCGGCACACTCCAGGACCGCCCTCCCAGTCCTTTCCGTGAGCCCCCAGGCACA GAGCGGGTACTAACAAGCAGCCCCTCTCGACAACTGGTGGGCAGAACATTTTCGGATGGGTTAGCAGCAA CTCGTACCCTCCAGCCGCCTGAGAGTCCCCGCCTGGGCCGGAGGGGCCTGGACAGTATGCGGGAACTGCC CCCGTTGAGTCCATCTCTGTCTCGAAGGGCTCTGTCCCCACTGCCAGCCCGGACTGCCCCAGATCCCAAG CTAAGCAGGGAAGTGGCCGAGAGTCCCCGGCCCCGGCGCTGGGCAGCCCACGGGACTTCACCTGAGGACT TCTCCCTGACTCTAGGAGCACGGGGTCGCAGGACTCGGAGTCCCTCGCCCACCCTCGGGGAGTCCCTGGC ACCTCGCAAGGGTAGCTTCAGTGGCAGGCTGAGCCCAGCTTACAGTCTGGGCTCTCTTACTGGGGCTTCA CCTCGTCAGAGCCCCCGTGCTCAGCGGAAGCTCTCCAGTGGGGACTTGCGGGTACCCATTCCAAGGGAGA GGAAAAACAGCATCACGGAGATCAGCGACAATGAGGACGAACTTCTGGAGTACCACCGTCGGCAGCGCCA AGAGCGGCTCCGGGAGCAGGAGATGGAGAGGCTGGAACGCCAGCGCCTGGAGACCATCCTGAACCTGTGT GCTGAGTACAGCCGAGCTGACGGGGGACCTGAGACTGGGGAGCTGCCCAGTATTGGAGAGGCCACGGCAG CGCTGGCCTTGGCAGGCAGGAGGCCCTCAAGAGGCTTGGCAGGAGCCATAGTAGTCTCCGGAAGGTGCGG CGAGGAGTCTGGAGGTGCCTCCCAGCGCCTGTGGGAGAGCATGGAGCGCTCTGATGAAGAGAATCTCAAA GAGGAATGCAGCAGCACCGAGAGCACCCAGCAGGAGCATGAAGACGCTCCTGGCGCGAAGCACCAAGGCG AGGTGCTGGCTGTGGAAGAGGAGCGGGCTCAGGTTCTGGGGCGAGTAGAACAGCTGAAGATCCGAGTGAA GGAGCTGGAACAGCAGCTGCAGGAGGCAGCCCGAGAGGCCGAGATGGAGAGAGCACTGCTGCAGGGGGAG CGGGAGGCAGAGCGAGCCTCGCTGCAGAAGGAACAAAGGGCGGTGGACCAGCTGCAGGAGAAGTTGGTGG CCTTGGAGACAGGCATCCAGAAGGACAGGGACAAGGAGGCAGACGCTCTAGAGACTGAGACAAAGCTCTT TGAGGACCTGGAGTTCCAGCAGCTGGAGCGTGAGAGTCGCGTGGAGGAGGAGCGGGAGCTGGCCGGCCAA GGGCTGCTGCGGAGCAAGGCTGAGTTGCTGCGCAGTGTGAGCAAGAGGAAGGAGCGCCTAGCTGTACTGG ACAGTCAGGCTGGGCAGATCCGGGCCCAGGCAGTACAGGAGTCAGAGCGTCTGGCCCGGGAAAAGAATGC TGCCCTGCAGCTACTGCAAAAGGAGAAGGAGAAGCTGAATGTCCTTGAGAGGAGATACCATTCGCTCACG GGGGGCAGGCCTTTTCCGAAGACCACCTCCACCCTCAAAGAGATGGAGAAGCTGCTGCTCCCTGCTGTAG ACTTAGAGCAGTGGTACCAGGAGTTGATGTCCGGGCTGGGGACTGGCCTTGCTGCAGCCTCCCCTCGCTC CTCTCCCCCGCCTTTGCCTGCCAAAGCTTCCCGTCAGCTGCAGGTTTACCGCTCCAAGATGGATAGTGAT GCGGCCAGCCCCCTGCCCAGGACCCGTAGCGGCCCTCTCCCCTCCTCCTCAGGCTCCTCCTCCTCCTCTT CACAGCTCAGCGTGGCTACCCTGGGCCGTAGTCCTTCCCCAAAGAGTGCTCTGCTCGCCCAGAATGGCAC CAGTAGCCTTCCTCGCAACCTGGCAGCCACACTGCAGGACATTGAGACCAAGCGCCAGCTGGCCCTACAG CAAAAGGGACACCAAGTGATCGAGGAGCAACGGAGGAGACTGGCGGAGCTGAAGCAGAAGGCGGCGGCAG AGGCTCAGTGCCAGTGGGATGCCTTGCATGGTGCAGGGCCCTTTTCAGCAGGCCCTTCAGGCTTCCCTGC ACTCATGCATCATTCCATCCTGCATCACCTGCCAGCTGGCCGGGAGCGTGGAGAGGAGGGCGAGCACGCT TACGACACCCTGAGCCTGGAGAGTTCAGACAGCATGGAGACCAGCATCTCTACAGGCGGCAACTCTGCCT GTTCCCCGGACAACATGTCCAGCGCCAGTGGTCTGGACATGGGCAAGATCGAGGAAATGGAGAAGATGCT AAAGGAAGCTCACGCAGAGAAGAGCCGGCTCATGGAGTCCAGGGAGCGGGAGATGGAGCTTCGGAGGCAG GCCCTGGAGGAGGAACGGAGGAGGCGGGAGCAAGTGGAGCGGCGGCTGCAGAGTGAGAGCGCCCGCAGGC AGCAGCTGGTGGAGAAAGAAGTCAAGCTCCGGGAAAAGCAGTTCTCCCAGGCACGGCCTCTTACACGTTA CCTTCCCAACCGGAAGGAGGACTTTGATCTGAAGACCCACATAGAGTCATCAGGCCACGGGGTGGACACC TGCCTACATGTGGTGCTTAGCAGCAAGGTGTGCCGTGGCTACCTGATCAAGATGGGTGGCAAGATTAAAT CGTGGAAGAAGCGCTGGTTTGTCTTTGATCGGCTGAAGCGCACCCTTTCCTATTACGTGGACAAACATGA GACGAAGCTGAAGGGGGTCATCTATTTCCAGGCCATCGAGGAAGTGTACTATGACCACCTGCGCAGCGCA GCCAAGAGTCCGAATCCGGCCCTCACCTTCTGTGTGAAGACCCATGACCGGTTGTACTACATGGTGGCCC CGTCTGCAGAGGCCATGCGCATCTGGATGGATGTCATCGTCACGGGGGCCGAGGGCTACACTCAATTCAT GAACTAACTACCCTGCACCTCCTGGGTTCCCCAGCTCAGCCCCAGGACTCCGTGCCCTGCCACCTGCCAC TCTGCTTGTCCCTGGGGACAGTGGCACATTGGGCACGGGGCTGCAAAGGATCGGGATGTAGTTGAGTACC TCGGGGCTTTTGTGCAAGAAGACAAATTGTGTTATGAGGAGTTTGGTGCTTGGGAATTGAGCTCCAGTCC CTAAAGAGCCAGCAGAGAGGAAAGGAAGAGGGAGAGGCCTTGTCGGTCCTGGGAGTCCAGAAGTTCAGGT TCCCTCCTGGGTCCAGCAGGCTGAAGAGGCTGCTGAGCTGCGCTCTCGGCTCTGTCCCGGCACCCACCCA CCCCAGCTTGTTTTCTCTTTGTAAAGGACAAACTATGGTACCAGAATCTGCCAAAGATCCCCTTCTCATC TCATCCCCTCTGCAGGGGCTGGGAGGGGTCCTGAGCAGAGCCACGTGCAGAGCTGGGGAGCGGCTCAGGC CCCAACCCCATCTGCCTCCTCCTCTTCTTCCTCTTCCTCCTCCTCCCACCTCCCACTTGTCACTACTCAG CTCAAGGACCAGAGACCTCAAGTGTCACTTTCGAGGTCCAGCCCCATCATCCTGTTACAAGCCCGTCATC CTTGTCTCCATGGTGACCGCTTCATCACCATGGTTACACACTAATTGCCATGGCCACTCTGTGGCTTTGC CCACTGCTTCTGGCTGTGGGCCACATGAGGGTACGTGCCATCATCTCTCCAACCCAGGCCTGGGGGCATG TCACATGGTGGGAGGAGATGGAATTCCTCCCGGCTGCAGTGTGTCTCCAGCCCCCAGCCCAGCCCCTCTC CCTTTTACTGTGCCTTGCTTAGAGCCAGAAGGGATGATGTCCTGGGATCCAAGACCAGAGGGACAGCATC ACTGAGGGGAGAGGAAGCTGAGAGGTCTGCCCTCCCATTCTAACAGGACTAGTTCCCAGTGAGGAACAAG GGTAGCTCAGAGTGCCTGCAAAGAAGCCTGAGTTGGGGCTGGGGCGGGCAGAAGCAGAGGCCCTGAGCTG TGGTAATCTCCCTTCAGTTTCCCAAAGGTGATGGGAAAGGAGAACGGAGGATAAGTCTCCAGCTGGCTGG CCTCAGCCTTGCGCCTTAACACTAAGCCACCTTCCCTGCCCTCCCCAGCACTGGGCCCTTAGTTGCTGGG CCTGGTTGGGTGTTTTGCAGTATTTGTAAGCATTTCAGCAGAACAATAAAAGCATTTTGACTATGTAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6842596 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6842596 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008603 same experiment
	EMAGE:31856 same embryo
	EMAGE:31317 same embryo
	EMAGE:31891 same embryo
	EMAGE:31893 same embryo
	EMAGE:30623 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source